Experiment: JK36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt zwf and edd are separated by 234 nucleotides edd and kdgA are separated by 36 nucleotides kdgA and purT are separated by 55 nucleotides
HEPCGN_04740: zwf - glucose-6-phosphate dehydrogenase, at 826,494 to 827,969
zwf
HEPCGN_04745: edd - phosphogluconate dehydratase, at 828,204 to 830,015
edd
HEPCGN_04750: kdgA - bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase, at 830,052 to 830,693
kdgA
HEPCGN_04755: purT - formate-dependent phosphoribosylglycinamide formyltransferase, at 830,749 to 831,927
purT
Position (kb) 828
829
830
831 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 827.355 kb on + strand, within zwf at 827.358 kb on - strand, within zwf at 827.493 kb on + strand, within zwf at 827.716 kb on - strand, within zwf at 827.729 kb on + strand, within zwf at 827.754 kb on + strand, within zwf at 827.787 kb on - strand, within zwf at 827.803 kb on + strand, within zwf at 827.804 kb on - strand, within zwf at 827.970 kb on + strand at 827.971 kb on - strand at 827.971 kb on - strand at 828.110 kb on - strand at 828.283 kb on + strand at 828.423 kb on + strand, within edd at 828.425 kb on + strand, within edd at 828.629 kb on + strand, within edd at 828.780 kb on - strand, within edd at 828.792 kb on + strand, within edd at 828.792 kb on + strand, within edd at 828.804 kb on - strand, within edd at 828.846 kb on + strand, within edd at 828.867 kb on - strand, within edd at 828.867 kb on - strand, within edd at 828.911 kb on + strand, within edd at 829.020 kb on - strand, within edd at 829.048 kb on + strand, within edd at 829.048 kb on + strand, within edd at 829.049 kb on - strand, within edd at 829.232 kb on + strand, within edd at 829.232 kb on + strand, within edd at 829.235 kb on + strand, within edd at 829.235 kb on + strand, within edd at 829.345 kb on - strand, within edd at 829.347 kb on - strand, within edd at 829.575 kb on - strand, within edd at 829.668 kb on - strand, within edd at 829.725 kb on - strand, within edd at 829.792 kb on - strand, within edd at 830.013 kb on + strand at 830.328 kb on - strand, within kdgA at 830.375 kb on + strand, within kdgA at 830.556 kb on + strand, within kdgA at 830.674 kb on + strand at 830.674 kb on + strand at 830.674 kb on + strand at 830.691 kb on + strand at 830.691 kb on + strand at 830.758 kb on + strand at 830.896 kb on + strand, within purT at 830.914 kb on - strand, within purT
Per-strain Table
Position Strand Gene LocusTag Fraction JK36 remove 827,355 + zwf HEPCGN_04740 0.58 -1.3 827,358 - zwf HEPCGN_04740 0.59 -0.8 827,493 + zwf HEPCGN_04740 0.68 -0.6 827,716 - zwf HEPCGN_04740 0.83 -1.7 827,729 + zwf HEPCGN_04740 0.84 -0.7 827,754 + zwf HEPCGN_04740 0.85 -1.5 827,787 - zwf HEPCGN_04740 0.88 -0.7 827,803 + zwf HEPCGN_04740 0.89 -1.3 827,804 - zwf HEPCGN_04740 0.89 -1.7 827,970 + -0.3 827,971 - -0.5 827,971 - -0.7 828,110 - -0.2 828,283 + -0.2 828,423 + edd HEPCGN_04745 0.12 +0.4 828,425 + edd HEPCGN_04745 0.12 +0.6 828,629 + edd HEPCGN_04745 0.23 -1.3 828,780 - edd HEPCGN_04745 0.32 +1.0 828,792 + edd HEPCGN_04745 0.32 +0.1 828,792 + edd HEPCGN_04745 0.32 +0.1 828,804 - edd HEPCGN_04745 0.33 +0.4 828,846 + edd HEPCGN_04745 0.35 +0.2 828,867 - edd HEPCGN_04745 0.37 +0.7 828,867 - edd HEPCGN_04745 0.37 -0.3 828,911 + edd HEPCGN_04745 0.39 +0.2 829,020 - edd HEPCGN_04745 0.45 +0.6 829,048 + edd HEPCGN_04745 0.47 +0.4 829,048 + edd HEPCGN_04745 0.47 +3.2 829,049 - edd HEPCGN_04745 0.47 +0.8 829,232 + edd HEPCGN_04745 0.57 +0.1 829,232 + edd HEPCGN_04745 0.57 +1.2 829,235 + edd HEPCGN_04745 0.57 -0.7 829,235 + edd HEPCGN_04745 0.57 +0.5 829,345 - edd HEPCGN_04745 0.63 +1.2 829,347 - edd HEPCGN_04745 0.63 +0.1 829,575 - edd HEPCGN_04745 0.76 -0.3 829,668 - edd HEPCGN_04745 0.81 +0.1 829,725 - edd HEPCGN_04745 0.84 +0.1 829,792 - edd HEPCGN_04745 0.88 -2.0 830,013 + -0.4 830,328 - kdgA HEPCGN_04750 0.43 +0.1 830,375 + kdgA HEPCGN_04750 0.50 -1.0 830,556 + kdgA HEPCGN_04750 0.79 -0.1 830,674 + +0.5 830,674 + +2.2 830,674 + +0.2 830,691 + +0.0 830,691 + -1.6 830,758 + -0.0 830,896 + purT HEPCGN_04755 0.12 +1.5 830,914 - purT HEPCGN_04755 0.14 +0.3
Or see this region's nucleotide sequence