Experiment: JK36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lptB and hlyD overlap by 8 nucleotides hlyD and cecR are separated by 2 nucleotides cecR and rhlE are separated by 228 nucleotides
HEPCGN_01735: lptB - LPS export ABC transporter ATP-binding protein, at 268,942 to 270,678
lptB
HEPCGN_01740: hlyD - secretion protein HlyD, at 270,671 to 271,666
hlyD
HEPCGN_01745: cecR - DNA-binding transcriptional regulator CecR, at 271,669 to 272,340
cecR
HEPCGN_01750: rhlE - ATP-dependent RNA helicase RhlE, at 272,569 to 273,930
rhlE
Position (kb) 271
272
273 Strain fitness (log2 ratio) -2
-1
0
1 at 270.754 kb on + strand at 270.880 kb on - strand, within hlyD at 270.880 kb on - strand, within hlyD at 270.880 kb on - strand, within hlyD at 270.919 kb on + strand, within hlyD at 270.933 kb on + strand, within hlyD at 270.970 kb on + strand, within hlyD at 270.975 kb on - strand, within hlyD at 270.992 kb on - strand, within hlyD at 271.046 kb on + strand, within hlyD at 271.155 kb on + strand, within hlyD at 271.155 kb on + strand, within hlyD at 271.168 kb on + strand, within hlyD at 271.278 kb on + strand, within hlyD at 271.278 kb on + strand, within hlyD at 271.278 kb on + strand, within hlyD at 271.279 kb on - strand, within hlyD at 271.293 kb on + strand, within hlyD at 271.293 kb on + strand, within hlyD at 271.560 kb on + strand, within hlyD at 271.628 kb on + strand at 271.629 kb on - strand at 271.631 kb on + strand at 271.631 kb on + strand at 271.632 kb on - strand at 271.635 kb on - strand at 271.671 kb on - strand at 271.711 kb on - strand at 271.774 kb on - strand, within cecR at 271.785 kb on + strand, within cecR at 271.836 kb on + strand, within cecR at 271.896 kb on + strand, within cecR at 271.896 kb on + strand, within cecR at 271.943 kb on + strand, within cecR at 272.019 kb on + strand, within cecR at 272.166 kb on - strand, within cecR at 272.166 kb on - strand, within cecR at 272.189 kb on + strand, within cecR at 272.193 kb on - strand, within cecR at 272.193 kb on - strand, within cecR at 272.334 kb on + strand at 272.372 kb on + strand at 272.373 kb on - strand at 272.388 kb on + strand at 272.440 kb on + strand at 272.469 kb on + strand at 272.470 kb on - strand at 272.568 kb on + strand at 272.860 kb on + strand, within rhlE at 273.151 kb on - strand, within rhlE at 273.283 kb on - strand, within rhlE
Per-strain Table
Position Strand Gene LocusTag Fraction JK36 remove 270,754 + +0.9 270,880 - hlyD HEPCGN_01740 0.21 -0.0 270,880 - hlyD HEPCGN_01740 0.21 -0.6 270,880 - hlyD HEPCGN_01740 0.21 +0.0 270,919 + hlyD HEPCGN_01740 0.25 +0.5 270,933 + hlyD HEPCGN_01740 0.26 +0.3 270,970 + hlyD HEPCGN_01740 0.30 -1.7 270,975 - hlyD HEPCGN_01740 0.31 -0.7 270,992 - hlyD HEPCGN_01740 0.32 -0.9 271,046 + hlyD HEPCGN_01740 0.38 -0.3 271,155 + hlyD HEPCGN_01740 0.49 +0.9 271,155 + hlyD HEPCGN_01740 0.49 +0.2 271,168 + hlyD HEPCGN_01740 0.50 +1.5 271,278 + hlyD HEPCGN_01740 0.61 +0.0 271,278 + hlyD HEPCGN_01740 0.61 +0.2 271,278 + hlyD HEPCGN_01740 0.61 -0.0 271,279 - hlyD HEPCGN_01740 0.61 +0.4 271,293 + hlyD HEPCGN_01740 0.62 +1.3 271,293 + hlyD HEPCGN_01740 0.62 -0.6 271,560 + hlyD HEPCGN_01740 0.89 -0.6 271,628 + +0.9 271,629 - +0.6 271,631 + +0.3 271,631 + -0.1 271,632 - -0.1 271,635 - -0.2 271,671 - +0.3 271,711 - -0.2 271,774 - cecR HEPCGN_01745 0.16 -0.3 271,785 + cecR HEPCGN_01745 0.17 -2.6 271,836 + cecR HEPCGN_01745 0.25 +0.1 271,896 + cecR HEPCGN_01745 0.34 +0.3 271,896 + cecR HEPCGN_01745 0.34 +1.7 271,943 + cecR HEPCGN_01745 0.41 +0.5 272,019 + cecR HEPCGN_01745 0.52 +0.6 272,166 - cecR HEPCGN_01745 0.74 -0.1 272,166 - cecR HEPCGN_01745 0.74 +0.9 272,189 + cecR HEPCGN_01745 0.77 -1.3 272,193 - cecR HEPCGN_01745 0.78 +1.0 272,193 - cecR HEPCGN_01745 0.78 +0.0 272,334 + -0.1 272,372 + -0.1 272,373 - +0.1 272,388 + -1.1 272,440 + -0.9 272,469 + +0.2 272,470 - -1.5 272,568 + -1.1 272,860 + rhlE HEPCGN_01750 0.21 +1.0 273,151 - rhlE HEPCGN_01750 0.43 -1.6 273,283 - rhlE HEPCGN_01750 0.52 -0.2
Or see this region's nucleotide sequence