Strain Fitness in Escherichia coli ECOR38 around HEPCGN_19760

Experiment: JK36

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthypB and hypA are separated by 3 nucleotideshypA and hycA are separated by 211 nucleotideshycA and hycB are separated by 124 nucleotideshycB and hycC overlap by 4 nucleotides HEPCGN_19750: hypB - hydrogenase nickel incorporation protein HypB, at 2,704,282 to 2,705,154 hypB HEPCGN_19755: hypA - hydrogenase maturation nickel metallochaperone HypA, at 2,705,158 to 2,705,508 hypA HEPCGN_19760: hycA - formate hydrogenlyase regulator HycA, at 2,705,720 to 2,706,181 hycA HEPCGN_19765: hycB - formate hydrogenlyase subunit HycB, at 2,706,306 to 2,706,917 hycB HEPCGN_19770: hycC - formate hydrogenlyase subunit 3, at 2,706,914 to 2,708,740 hycC Position (kb) 2705 2706 2707Strain fitness (log2 ratio) -3 -2 -1 0 1at 2705.097 kb on + strandat 2705.098 kb on - strandat 2705.117 kb on + strandat 2705.117 kb on + strandat 2705.118 kb on - strandat 2705.148 kb on + strandat 2705.149 kb on - strandat 2705.170 kb on + strandat 2705.171 kb on - strandat 2705.191 kb on + strandat 2705.264 kb on - strand, within hypAat 2705.273 kb on + strand, within hypAat 2705.273 kb on + strand, within hypAat 2705.274 kb on - strand, within hypAat 2705.274 kb on - strand, within hypAat 2705.328 kb on + strand, within hypAat 2705.329 kb on - strand, within hypAat 2705.726 kb on - strandat 2705.736 kb on + strandat 2705.737 kb on - strandat 2705.798 kb on + strand, within hycAat 2705.912 kb on + strand, within hycAat 2705.913 kb on - strand, within hycAat 2705.957 kb on + strand, within hycAat 2706.074 kb on + strand, within hycAat 2706.075 kb on - strand, within hycAat 2706.108 kb on + strand, within hycAat 2706.108 kb on + strand, within hycAat 2706.108 kb on + strand, within hycAat 2706.108 kb on + strand, within hycAat 2706.108 kb on + strand, within hycAat 2706.109 kb on - strand, within hycAat 2706.109 kb on - strand, within hycAat 2706.109 kb on - strand, within hycAat 2706.109 kb on - strand, within hycAat 2706.109 kb on - strand, within hycAat 2706.111 kb on - strand, within hycAat 2706.112 kb on + strand, within hycAat 2706.156 kb on + strandat 2706.554 kb on - strand, within hycBat 2706.554 kb on - strand, within hycBat 2706.710 kb on + strand, within hycBat 2706.839 kb on + strand, within hycBat 2706.839 kb on + strand, within hycBat 2706.857 kb on + strandat 2706.857 kb on + strandat 2706.911 kb on + strandat 2706.912 kb on - strandat 2707.047 kb on - strandat 2707.119 kb on - strand, within hycCat 2707.159 kb on + strand, within hycCat 2707.159 kb on + strand, within hycC

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Per-strain Table

Position Strand Gene LocusTag Fraction JK36
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2,705,097 + -0.4
2,705,098 - +0.2
2,705,117 + -1.7
2,705,117 + -0.0
2,705,118 - -0.1
2,705,148 + -0.2
2,705,149 - +0.3
2,705,170 + -0.9
2,705,171 - +0.3
2,705,191 + -0.6
2,705,264 - hypA HEPCGN_19755 0.30 -2.9
2,705,273 + hypA HEPCGN_19755 0.33 -0.1
2,705,273 + hypA HEPCGN_19755 0.33 +0.0
2,705,274 - hypA HEPCGN_19755 0.33 +0.4
2,705,274 - hypA HEPCGN_19755 0.33 -0.1
2,705,328 + hypA HEPCGN_19755 0.48 +0.0
2,705,329 - hypA HEPCGN_19755 0.49 +0.4
2,705,726 - +0.4
2,705,736 + +0.0
2,705,737 - +0.3
2,705,798 + hycA HEPCGN_19760 0.17 +0.3
2,705,912 + hycA HEPCGN_19760 0.42 -0.8
2,705,913 - hycA HEPCGN_19760 0.42 -1.9
2,705,957 + hycA HEPCGN_19760 0.51 -0.5
2,706,074 + hycA HEPCGN_19760 0.77 +0.6
2,706,075 - hycA HEPCGN_19760 0.77 -1.7
2,706,108 + hycA HEPCGN_19760 0.84 -1.0
2,706,108 + hycA HEPCGN_19760 0.84 -0.8
2,706,108 + hycA HEPCGN_19760 0.84 -0.6
2,706,108 + hycA HEPCGN_19760 0.84 -2.9
2,706,108 + hycA HEPCGN_19760 0.84 -0.3
2,706,109 - hycA HEPCGN_19760 0.84 +0.2
2,706,109 - hycA HEPCGN_19760 0.84 -0.5
2,706,109 - hycA HEPCGN_19760 0.84 +0.3
2,706,109 - hycA HEPCGN_19760 0.84 -1.1
2,706,109 - hycA HEPCGN_19760 0.84 -0.0
2,706,111 - hycA HEPCGN_19760 0.85 -0.1
2,706,112 + hycA HEPCGN_19760 0.85 +0.1
2,706,156 + -0.4
2,706,554 - hycB HEPCGN_19765 0.41 +1.2
2,706,554 - hycB HEPCGN_19765 0.41 +0.3
2,706,710 + hycB HEPCGN_19765 0.66 -0.4
2,706,839 + hycB HEPCGN_19765 0.87 -0.2
2,706,839 + hycB HEPCGN_19765 0.87 -0.1
2,706,857 + -0.4
2,706,857 + -1.0
2,706,911 + +0.6
2,706,912 - +0.6
2,707,047 - -0.6
2,707,119 - hycC HEPCGN_19770 0.11 +0.4
2,707,159 + hycC HEPCGN_19770 0.13 -0.8
2,707,159 + hycC HEPCGN_19770 0.13 -1.5

Or see this region's nucleotide sequence