Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prfB and lysS are separated by 9 nucleotides lysS and idi are separated by 42 nucleotides idi and yqfG are separated by 121 nucleotides yqfG and uacT are separated by 1 nucleotides
HEPCGN_18875: prfB - peptide chain release factor 2, at 2,519,115 to 2,519,996
prfB
HEPCGN_18880: lysS - lysine--tRNA ligase, at 2,520,006 to 2,521,523
lysS
HEPCGN_18885: idi - isopentenyl-diphosphate Delta-isomerase, at 2,521,566 to 2,522,114
idi
HEPCGN_18890: yqfG - Uncharacterized protein YqfG, at 2,522,236 to 2,522,361
yqfG
HEPCGN_18895: uacT - urate/proton symporter UacT, at 2,522,363 to 2,523,811
uacT
Position (kb)
2520
2521
2522 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2519.993 kb on - strand at 2520.240 kb on - strand, within lysS at 2520.298 kb on - strand, within lysS at 2520.298 kb on - strand, within lysS at 2520.557 kb on + strand, within lysS at 2520.708 kb on + strand, within lysS at 2520.709 kb on - strand, within lysS at 2520.716 kb on + strand, within lysS at 2520.846 kb on + strand, within lysS at 2520.846 kb on + strand, within lysS at 2520.847 kb on - strand, within lysS at 2520.847 kb on - strand, within lysS at 2521.374 kb on + strand at 2521.453 kb on + strand at 2521.476 kb on - strand at 2521.528 kb on + strand at 2521.529 kb on - strand at 2521.529 kb on - strand at 2521.529 kb on - strand at 2521.534 kb on - strand at 2521.672 kb on - strand, within idi at 2521.674 kb on + strand, within idi at 2521.674 kb on + strand, within idi at 2521.675 kb on - strand, within idi at 2521.699 kb on + strand, within idi at 2521.820 kb on - strand, within idi at 2521.840 kb on - strand, within idi at 2521.974 kb on + strand, within idi at 2521.974 kb on + strand, within idi at 2521.974 kb on + strand, within idi at 2521.975 kb on - strand, within idi at 2521.978 kb on - strand, within idi at 2521.978 kb on - strand, within idi at 2521.978 kb on - strand, within idi at 2522.023 kb on - strand, within idi at 2522.050 kb on + strand, within idi at 2522.114 kb on + strand at 2522.128 kb on + strand at 2522.129 kb on - strand at 2522.151 kb on - strand at 2522.228 kb on - strand at 2522.253 kb on + strand, within yqfG at 2522.254 kb on - strand, within yqfG at 2522.254 kb on - strand, within yqfG at 2522.317 kb on - strand, within yqfG at 2522.361 kb on + strand at 2522.365 kb on - strand at 2522.410 kb on + strand at 2522.410 kb on + strand at 2522.410 kb on + strand at 2522.410 kb on + strand at 2522.411 kb on - strand at 2522.415 kb on - strand at 2522.415 kb on - strand at 2522.415 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 2,519,993 - +1.8 2,520,240 - lysS HEPCGN_18880 0.15 -2.2 2,520,298 - lysS HEPCGN_18880 0.19 -0.2 2,520,298 - lysS HEPCGN_18880 0.19 +0.3 2,520,557 + lysS HEPCGN_18880 0.36 +2.2 2,520,708 + lysS HEPCGN_18880 0.46 -0.4 2,520,709 - lysS HEPCGN_18880 0.46 -1.6 2,520,716 + lysS HEPCGN_18880 0.47 -0.9 2,520,846 + lysS HEPCGN_18880 0.55 -0.9 2,520,846 + lysS HEPCGN_18880 0.55 -0.8 2,520,847 - lysS HEPCGN_18880 0.55 -2.8 2,520,847 - lysS HEPCGN_18880 0.55 -0.6 2,521,374 + -1.7 2,521,453 + +0.7 2,521,476 - -2.2 2,521,528 + -0.7 2,521,529 - -1.7 2,521,529 - +0.3 2,521,529 - -0.9 2,521,534 - -0.6 2,521,672 - idi HEPCGN_18885 0.19 -0.6 2,521,674 + idi HEPCGN_18885 0.20 +0.1 2,521,674 + idi HEPCGN_18885 0.20 +2.6 2,521,675 - idi HEPCGN_18885 0.20 -0.4 2,521,699 + idi HEPCGN_18885 0.24 -0.0 2,521,820 - idi HEPCGN_18885 0.46 -0.6 2,521,840 - idi HEPCGN_18885 0.50 -0.2 2,521,974 + idi HEPCGN_18885 0.74 -1.0 2,521,974 + idi HEPCGN_18885 0.74 -0.9 2,521,974 + idi HEPCGN_18885 0.74 +1.1 2,521,975 - idi HEPCGN_18885 0.74 +0.4 2,521,978 - idi HEPCGN_18885 0.75 +0.5 2,521,978 - idi HEPCGN_18885 0.75 +0.7 2,521,978 - idi HEPCGN_18885 0.75 -0.1 2,522,023 - idi HEPCGN_18885 0.83 +0.8 2,522,050 + idi HEPCGN_18885 0.88 +0.8 2,522,114 + +0.3 2,522,128 + -0.7 2,522,129 - -0.1 2,522,151 - +1.3 2,522,228 - +1.9 2,522,253 + yqfG HEPCGN_18890 0.13 -0.0 2,522,254 - yqfG HEPCGN_18890 0.14 +0.3 2,522,254 - yqfG HEPCGN_18890 0.14 -0.6 2,522,317 - yqfG HEPCGN_18890 0.64 -0.1 2,522,361 + +0.2 2,522,365 - -0.6 2,522,410 + -0.1 2,522,410 + +1.1 2,522,410 + +0.1 2,522,410 + +0.1 2,522,411 - +0.0 2,522,415 - -0.9 2,522,415 - -0.5 2,522,415 - -1.7
Or see this region's nucleotide sequence