Strain Fitness in Escherichia coli ECOR38 around HEPCGN_12350

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmetB and metJ are separated by 276 nucleotidesmetJ and yiiX are separated by 59 nucleotidesyiiX and rpmE are separated by 60 nucleotides HEPCGN_12345: metB - cystathionine gamma-synthase, at 1,220,062 to 1,221,222 metB HEPCGN_12350: metJ - met regulon transcriptional regulator MetJ, at 1,221,499 to 1,221,816 metJ HEPCGN_12355: yiiX - YiiX family permuted papain-like enzyme, at 1,221,876 to 1,222,484 yiiX HEPCGN_12360: rpmE - 50S ribosomal protein L31, at 1,222,545 to 1,222,757 rpmE Position (kb) 1221 1222Strain fitness (log2 ratio) -2 -1 0 1 2at 1220.507 kb on + strand, within metBat 1220.507 kb on + strand, within metBat 1220.634 kb on + strand, within metBat 1220.663 kb on - strand, within metBat 1220.663 kb on - strand, within metBat 1220.681 kb on - strand, within metBat 1220.693 kb on - strand, within metBat 1220.693 kb on - strand, within metBat 1220.782 kb on - strand, within metBat 1220.794 kb on + strand, within metBat 1221.004 kb on - strand, within metBat 1221.108 kb on + strandat 1221.124 kb on - strandat 1221.125 kb on + strandat 1221.126 kb on - strandat 1221.126 kb on - strandat 1221.165 kb on - strandat 1221.271 kb on - strandat 1221.271 kb on - strandat 1221.296 kb on - strandat 1221.346 kb on - strandat 1221.367 kb on + strandat 1221.387 kb on + strandat 1221.421 kb on + strandat 1221.421 kb on + strandat 1221.421 kb on + strandat 1221.422 kb on - strandat 1221.432 kb on - strandat 1221.489 kb on + strandat 1221.489 kb on + strandat 1221.490 kb on - strandat 1221.490 kb on - strandat 1221.493 kb on + strandat 1221.494 kb on - strandat 1221.494 kb on - strandat 1221.520 kb on + strandat 1221.520 kb on + strandat 1221.521 kb on - strandat 1221.521 kb on - strandat 1221.521 kb on - strandat 1221.523 kb on - strandat 1221.523 kb on - strandat 1221.532 kb on + strand, within metJat 1221.592 kb on - strand, within metJat 1221.859 kb on + strandat 1221.871 kb on + strandat 1221.872 kb on - strandat 1222.022 kb on + strand, within yiiXat 1222.022 kb on + strand, within yiiXat 1222.028 kb on + strand, within yiiXat 1222.029 kb on - strand, within yiiXat 1222.057 kb on - strand, within yiiXat 1222.086 kb on + strand, within yiiXat 1222.087 kb on - strand, within yiiXat 1222.131 kb on + strand, within yiiXat 1222.132 kb on - strand, within yiiXat 1222.132 kb on - strand, within yiiXat 1222.132 kb on - strand, within yiiXat 1222.218 kb on + strand, within yiiXat 1222.218 kb on + strand, within yiiXat 1222.219 kb on - strand, within yiiXat 1222.227 kb on - strand, within yiiXat 1222.281 kb on + strand, within yiiXat 1222.409 kb on - strand, within yiiXat 1222.485 kb on + strandat 1222.485 kb on + strandat 1222.547 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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1,220,507 + metB HEPCGN_12345 0.38 +0.2
1,220,507 + metB HEPCGN_12345 0.38 +0.2
1,220,634 + metB HEPCGN_12345 0.49 -1.3
1,220,663 - metB HEPCGN_12345 0.52 +0.7
1,220,663 - metB HEPCGN_12345 0.52 +0.6
1,220,681 - metB HEPCGN_12345 0.53 +1.0
1,220,693 - metB HEPCGN_12345 0.54 -0.1
1,220,693 - metB HEPCGN_12345 0.54 +0.1
1,220,782 - metB HEPCGN_12345 0.62 -0.8
1,220,794 + metB HEPCGN_12345 0.63 +0.3
1,221,004 - metB HEPCGN_12345 0.81 +0.0
1,221,108 + +0.4
1,221,124 - -1.5
1,221,125 + +0.7
1,221,126 - -1.2
1,221,126 - +0.1
1,221,165 - -1.2
1,221,271 - -0.8
1,221,271 - -0.4
1,221,296 - -0.5
1,221,346 - +0.2
1,221,367 + +0.6
1,221,387 + +0.2
1,221,421 + +0.2
1,221,421 + +0.6
1,221,421 + -0.1
1,221,422 - -1.2
1,221,432 - -1.5
1,221,489 + -0.2
1,221,489 + -1.0
1,221,490 - -0.8
1,221,490 - -2.2
1,221,493 + -1.8
1,221,494 - -0.9
1,221,494 - -0.7
1,221,520 + -2.2
1,221,520 + -2.2
1,221,521 - -2.4
1,221,521 - -0.9
1,221,521 - -0.0
1,221,523 - +0.3
1,221,523 - +1.5
1,221,532 + metJ HEPCGN_12350 0.10 -2.6
1,221,592 - metJ HEPCGN_12350 0.29 -1.8
1,221,859 + -1.4
1,221,871 + +0.2
1,221,872 - -2.6
1,222,022 + yiiX HEPCGN_12355 0.24 -1.0
1,222,022 + yiiX HEPCGN_12355 0.24 +0.5
1,222,028 + yiiX HEPCGN_12355 0.25 +0.6
1,222,029 - yiiX HEPCGN_12355 0.25 -1.9
1,222,057 - yiiX HEPCGN_12355 0.30 -0.6
1,222,086 + yiiX HEPCGN_12355 0.34 +0.2
1,222,087 - yiiX HEPCGN_12355 0.35 +1.9
1,222,131 + yiiX HEPCGN_12355 0.42 +0.8
1,222,132 - yiiX HEPCGN_12355 0.42 -0.1
1,222,132 - yiiX HEPCGN_12355 0.42 -0.3
1,222,132 - yiiX HEPCGN_12355 0.42 -1.2
1,222,218 + yiiX HEPCGN_12355 0.56 +0.4
1,222,218 + yiiX HEPCGN_12355 0.56 -0.0
1,222,219 - yiiX HEPCGN_12355 0.56 -0.4
1,222,227 - yiiX HEPCGN_12355 0.58 +0.7
1,222,281 + yiiX HEPCGN_12355 0.67 +0.5
1,222,409 - yiiX HEPCGN_12355 0.88 +2.2
1,222,485 + -0.7
1,222,485 + -0.4
1,222,547 - +0.6

Or see this region's nucleotide sequence