Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lysS and ghoT are separated by 118 nucleotides ghoT and ghoS are separated by 27 nucleotides ghoS and yjdJ are separated by 228 nucleotides yjdJ and yjdI are separated by 11 nucleotides yjdI and dcuS are separated by 180 nucleotides
HEPCGN_11120: lysS - lysine--tRNA ligase, at 963,122 to 964,639
lysS
HEPCGN_11125: ghoT - type V toxin-antitoxin system toxin GhoT, at 964,758 to 964,931
ghoT
HEPCGN_11130: ghoS - type V toxin-antitoxin system endoribonuclease antitoxin GhoS, at 964,959 to 965,255
ghoS
HEPCGN_11135: yjdJ - Uncharacterized protein YjdJ, at 965,484 to 965,756
yjdJ
HEPCGN_11140: yjdI - Uncharacterized protein YjdI, at 965,768 to 965,998
yjdI
HEPCGN_11145: dcuS - Sensor histidine kinase DcuS, at 966,179 to 967,810
dcuS
Position (kb) 965
966 Strain fitness (log2 ratio) -2
-1
0
1
2 at 964.490 kb on + strand at 964.491 kb on - strand at 964.491 kb on - strand at 964.503 kb on - strand at 964.520 kb on + strand at 964.521 kb on - strand at 964.591 kb on + strand at 964.591 kb on + strand at 964.594 kb on + strand at 964.595 kb on - strand at 964.788 kb on + strand, within ghoT at 964.826 kb on + strand, within ghoT at 964.826 kb on + strand, within ghoT at 964.826 kb on + strand, within ghoT at 964.827 kb on - strand, within ghoT at 964.900 kb on + strand, within ghoT at 965.031 kb on + strand, within ghoS at 965.050 kb on - strand, within ghoS at 965.273 kb on + strand at 965.301 kb on - strand at 965.645 kb on + strand, within yjdJ at 965.769 kb on + strand at 965.782 kb on - strand at 965.879 kb on + strand, within yjdI at 965.922 kb on - strand, within yjdI at 965.932 kb on + strand, within yjdI at 966.020 kb on - strand at 966.033 kb on + strand at 966.033 kb on + strand at 966.033 kb on + strand at 966.033 kb on + strand at 966.033 kb on + strand at 966.034 kb on - strand at 966.034 kb on - strand at 966.034 kb on - strand at 966.039 kb on + strand at 966.057 kb on - strand at 966.095 kb on + strand at 966.095 kb on + strand at 966.096 kb on - strand at 966.097 kb on + strand at 966.098 kb on - strand at 966.113 kb on + strand at 966.145 kb on + strand at 966.146 kb on - strand at 966.205 kb on - strand at 966.246 kb on + strand at 966.276 kb on + strand at 966.297 kb on - strand at 966.317 kb on - strand at 966.524 kb on - strand, within dcuS at 966.524 kb on - strand, within dcuS at 966.556 kb on + strand, within dcuS at 966.556 kb on + strand, within dcuS at 966.557 kb on - strand, within dcuS at 966.615 kb on + strand, within dcuS at 966.621 kb on + strand, within dcuS at 966.622 kb on - strand, within dcuS at 966.622 kb on - strand, within dcuS at 966.636 kb on - strand, within dcuS at 966.636 kb on - strand, within dcuS at 966.636 kb on - strand, within dcuS at 966.636 kb on - strand, within dcuS at 966.741 kb on + strand, within dcuS at 966.742 kb on - strand, within dcuS
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 964,490 + +0.2 964,491 - -0.4 964,491 - +0.1 964,503 - +1.1 964,520 + -0.5 964,521 - +0.1 964,591 + +0.3 964,591 + -0.7 964,594 + -0.2 964,595 - +1.3 964,788 + ghoT HEPCGN_11125 0.17 +0.6 964,826 + ghoT HEPCGN_11125 0.39 +0.1 964,826 + ghoT HEPCGN_11125 0.39 -0.5 964,826 + ghoT HEPCGN_11125 0.39 +0.1 964,827 - ghoT HEPCGN_11125 0.40 -0.7 964,900 + ghoT HEPCGN_11125 0.82 -0.1 965,031 + ghoS HEPCGN_11130 0.24 -0.3 965,050 - ghoS HEPCGN_11130 0.31 +0.9 965,273 + -1.0 965,301 - +0.7 965,645 + yjdJ HEPCGN_11135 0.59 -1.3 965,769 + +0.3 965,782 - +0.1 965,879 + yjdI HEPCGN_11140 0.48 +0.2 965,922 - yjdI HEPCGN_11140 0.67 +0.4 965,932 + yjdI HEPCGN_11140 0.71 -0.7 966,020 - +0.1 966,033 + -0.3 966,033 + +0.6 966,033 + +0.1 966,033 + +0.1 966,033 + -0.7 966,034 - +0.8 966,034 - -0.4 966,034 - -0.3 966,039 + -0.2 966,057 - -0.7 966,095 + +1.1 966,095 + -0.0 966,096 - -0.2 966,097 + +0.6 966,098 - +0.2 966,113 + +0.1 966,145 + -2.7 966,146 - +0.2 966,205 - -0.3 966,246 + -0.2 966,276 + -1.0 966,297 - -0.6 966,317 - +0.9 966,524 - dcuS HEPCGN_11145 0.21 -0.1 966,524 - dcuS HEPCGN_11145 0.21 -0.4 966,556 + dcuS HEPCGN_11145 0.23 +0.8 966,556 + dcuS HEPCGN_11145 0.23 -0.9 966,557 - dcuS HEPCGN_11145 0.23 -0.0 966,615 + dcuS HEPCGN_11145 0.27 +2.2 966,621 + dcuS HEPCGN_11145 0.27 +0.4 966,622 - dcuS HEPCGN_11145 0.27 +1.1 966,622 - dcuS HEPCGN_11145 0.27 -0.7 966,636 - dcuS HEPCGN_11145 0.28 -0.6 966,636 - dcuS HEPCGN_11145 0.28 +0.1 966,636 - dcuS HEPCGN_11145 0.28 +0.2 966,636 - dcuS HEPCGN_11145 0.28 -1.8 966,741 + dcuS HEPCGN_11145 0.34 -0.1 966,742 - dcuS HEPCGN_11145 0.34 -0.4
Or see this region's nucleotide sequence