Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt intQ and HEPCGN_06205 are separated by 357 nucleotides HEPCGN_06205 and tnp-IS629 are separated by 103 nucleotides tnp-IS629 and tnp-IS3 overlap by 1 nucleotides
HEPCGN_06200: intQ - Putative defective protein IntQ, at 1,113,822 to 1,115,102
intQ
HEPCGN_06205: HEPCGN_06205 - DUF5051 domain-containing protein, at 1,115,460 to 1,116,002
_06205
HEPCGN_06210: tnp-IS629 - IS3 family IS629 transposase, at 1,116,106 to 1,116,432
tnp-IS629
HEPCGN_06215: tnp-IS3 - IS3 family transposase, at 1,116,432 to 1,117,319
tnp-IS3
Position (kb)
1115
1116
1117 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1114.570 kb on + strand, within intQ at 1114.622 kb on + strand, within intQ at 1114.727 kb on + strand, within intQ at 1114.972 kb on - strand, within intQ at 1115.042 kb on + strand at 1115.058 kb on - strand at 1115.118 kb on + strand at 1115.207 kb on + strand at 1115.208 kb on - strand at 1115.256 kb on + strand at 1115.256 kb on + strand at 1115.256 kb on + strand at 1115.257 kb on - strand at 1115.259 kb on - strand at 1115.300 kb on - strand at 1115.305 kb on - strand at 1115.331 kb on + strand at 1115.376 kb on + strand at 1115.377 kb on - strand at 1115.377 kb on - strand at 1115.397 kb on + strand at 1115.404 kb on + strand at 1115.404 kb on + strand at 1115.405 kb on - strand at 1115.770 kb on - strand, within HEPCGN_06205 at 1116.079 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 1,114,570 + intQ HEPCGN_06200 0.58 +0.5 1,114,622 + intQ HEPCGN_06200 0.62 +1.3 1,114,727 + intQ HEPCGN_06200 0.71 +0.1 1,114,972 - intQ HEPCGN_06200 0.90 -0.6 1,115,042 + -0.2 1,115,058 - +0.8 1,115,118 + +1.3 1,115,207 + -1.8 1,115,208 - +0.3 1,115,256 + +3.2 1,115,256 + +1.1 1,115,256 + +1.0 1,115,257 - +0.0 1,115,259 - +0.9 1,115,300 - +0.8 1,115,305 - -1.2 1,115,331 + +0.1 1,115,376 + -1.0 1,115,377 - -0.9 1,115,377 - +0.9 1,115,397 + -1.0 1,115,404 + -0.2 1,115,404 + +0.3 1,115,405 - +0.5 1,115,770 - HEPCGN_06205 0.57 -0.8 1,116,079 - +0.2
Or see this region's nucleotide sequence