Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydgA and manA are separated by 100 nucleotides manA and fumB are separated by 198 nucleotides
HEPCGN_06010: ydgA - Protein YdgA, at 1,075,191 to 1,076,699
ydgA
HEPCGN_06015: manA - mannose-6-phosphate isomerase, at 1,076,800 to 1,077,975
manA
HEPCGN_06020: fumB - class I fumarate hydratase, at 1,078,174 to 1,079,820
fumB
Position (kb)
1076
1077
1078 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1076.231 kb on + strand, within ydgA at 1076.270 kb on + strand, within ydgA at 1076.271 kb on - strand, within ydgA at 1076.652 kb on + strand at 1076.693 kb on + strand at 1076.749 kb on + strand at 1076.751 kb on + strand at 1076.752 kb on - strand at 1076.752 kb on - strand at 1076.893 kb on - strand at 1076.986 kb on - strand, within manA at 1077.127 kb on - strand, within manA at 1077.600 kb on - strand, within manA at 1077.600 kb on - strand, within manA at 1077.600 kb on - strand, within manA at 1077.909 kb on - strand at 1077.944 kb on + strand at 1077.992 kb on - strand at 1078.016 kb on - strand at 1078.228 kb on - strand at 1078.234 kb on + strand at 1078.238 kb on - strand at 1078.265 kb on + strand at 1078.313 kb on + strand at 1078.314 kb on - strand at 1078.422 kb on + strand, within fumB at 1078.426 kb on + strand, within fumB at 1078.427 kb on - strand, within fumB at 1078.509 kb on + strand, within fumB at 1078.582 kb on - strand, within fumB at 1078.589 kb on - strand, within fumB at 1078.589 kb on - strand, within fumB at 1078.589 kb on - strand, within fumB at 1078.589 kb on - strand, within fumB at 1078.589 kb on - strand, within fumB at 1078.636 kb on + strand, within fumB at 1078.636 kb on + strand, within fumB at 1078.636 kb on + strand, within fumB at 1078.636 kb on + strand, within fumB at 1078.636 kb on + strand, within fumB at 1078.637 kb on - strand, within fumB at 1078.637 kb on - strand, within fumB at 1078.638 kb on + strand, within fumB at 1078.664 kb on + strand, within fumB at 1078.664 kb on + strand, within fumB at 1078.685 kb on - strand, within fumB at 1078.685 kb on - strand, within fumB at 1078.753 kb on + strand, within fumB at 1078.754 kb on - strand, within fumB at 1078.853 kb on - strand, within fumB at 1078.857 kb on + strand, within fumB at 1078.857 kb on + strand, within fumB at 1078.858 kb on - strand, within fumB at 1078.900 kb on - strand, within fumB at 1078.900 kb on - strand, within fumB at 1078.923 kb on + strand, within fumB at 1078.927 kb on + strand, within fumB at 1078.930 kb on + strand, within fumB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 1,076,231 + ydgA HEPCGN_06010 0.69 +0.9 1,076,270 + ydgA HEPCGN_06010 0.72 +0.2 1,076,271 - ydgA HEPCGN_06010 0.72 +0.0 1,076,652 + +2.1 1,076,693 + -1.1 1,076,749 + +0.9 1,076,751 + +0.1 1,076,752 - +2.3 1,076,752 - +0.2 1,076,893 - +1.2 1,076,986 - manA HEPCGN_06015 0.16 -0.5 1,077,127 - manA HEPCGN_06015 0.28 -0.3 1,077,600 - manA HEPCGN_06015 0.68 +1.0 1,077,600 - manA HEPCGN_06015 0.68 -0.9 1,077,600 - manA HEPCGN_06015 0.68 -1.2 1,077,909 - -0.6 1,077,944 + +2.6 1,077,992 - +0.2 1,078,016 - +0.1 1,078,228 - -0.9 1,078,234 + +0.2 1,078,238 - +0.3 1,078,265 + +0.0 1,078,313 + +0.6 1,078,314 - +0.8 1,078,422 + fumB HEPCGN_06020 0.15 +0.4 1,078,426 + fumB HEPCGN_06020 0.15 -1.0 1,078,427 - fumB HEPCGN_06020 0.15 -0.2 1,078,509 + fumB HEPCGN_06020 0.20 +0.0 1,078,582 - fumB HEPCGN_06020 0.25 +1.7 1,078,589 - fumB HEPCGN_06020 0.25 +1.9 1,078,589 - fumB HEPCGN_06020 0.25 -0.3 1,078,589 - fumB HEPCGN_06020 0.25 +0.5 1,078,589 - fumB HEPCGN_06020 0.25 -0.7 1,078,589 - fumB HEPCGN_06020 0.25 +0.2 1,078,636 + fumB HEPCGN_06020 0.28 +0.3 1,078,636 + fumB HEPCGN_06020 0.28 +0.7 1,078,636 + fumB HEPCGN_06020 0.28 -0.6 1,078,636 + fumB HEPCGN_06020 0.28 +0.1 1,078,636 + fumB HEPCGN_06020 0.28 -0.6 1,078,637 - fumB HEPCGN_06020 0.28 -1.3 1,078,637 - fumB HEPCGN_06020 0.28 +0.4 1,078,638 + fumB HEPCGN_06020 0.28 -0.5 1,078,664 + fumB HEPCGN_06020 0.30 +1.1 1,078,664 + fumB HEPCGN_06020 0.30 +0.3 1,078,685 - fumB HEPCGN_06020 0.31 +3.2 1,078,685 - fumB HEPCGN_06020 0.31 -2.7 1,078,753 + fumB HEPCGN_06020 0.35 -0.3 1,078,754 - fumB HEPCGN_06020 0.35 +2.2 1,078,853 - fumB HEPCGN_06020 0.41 -0.1 1,078,857 + fumB HEPCGN_06020 0.41 -2.1 1,078,857 + fumB HEPCGN_06020 0.41 +1.0 1,078,858 - fumB HEPCGN_06020 0.42 -0.1 1,078,900 - fumB HEPCGN_06020 0.44 -1.4 1,078,900 - fumB HEPCGN_06020 0.44 -0.1 1,078,923 + fumB HEPCGN_06020 0.45 +0.3 1,078,927 + fumB HEPCGN_06020 0.46 -0.5 1,078,930 + fumB HEPCGN_06020 0.46 -0.7
Or see this region's nucleotide sequence