Strain Fitness in Escherichia coli ECOR38 around HEPCGN_06015

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydgA and manA are separated by 100 nucleotidesmanA and fumB are separated by 198 nucleotides HEPCGN_06010: ydgA - Protein YdgA, at 1,075,191 to 1,076,699 ydgA HEPCGN_06015: manA - mannose-6-phosphate isomerase, at 1,076,800 to 1,077,975 manA HEPCGN_06020: fumB - class I fumarate hydratase, at 1,078,174 to 1,079,820 fumB Position (kb) 1076 1077 1078Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1076.231 kb on + strand, within ydgAat 1076.270 kb on + strand, within ydgAat 1076.271 kb on - strand, within ydgAat 1076.652 kb on + strandat 1076.693 kb on + strandat 1076.749 kb on + strandat 1076.751 kb on + strandat 1076.752 kb on - strandat 1076.752 kb on - strandat 1076.893 kb on - strandat 1076.986 kb on - strand, within manAat 1077.127 kb on - strand, within manAat 1077.600 kb on - strand, within manAat 1077.600 kb on - strand, within manAat 1077.600 kb on - strand, within manAat 1077.909 kb on - strandat 1077.944 kb on + strandat 1077.992 kb on - strandat 1078.016 kb on - strandat 1078.228 kb on - strandat 1078.234 kb on + strandat 1078.238 kb on - strandat 1078.265 kb on + strandat 1078.313 kb on + strandat 1078.314 kb on - strandat 1078.422 kb on + strand, within fumBat 1078.426 kb on + strand, within fumBat 1078.427 kb on - strand, within fumBat 1078.509 kb on + strand, within fumBat 1078.582 kb on - strand, within fumBat 1078.589 kb on - strand, within fumBat 1078.589 kb on - strand, within fumBat 1078.589 kb on - strand, within fumBat 1078.589 kb on - strand, within fumBat 1078.589 kb on - strand, within fumBat 1078.636 kb on + strand, within fumBat 1078.636 kb on + strand, within fumBat 1078.636 kb on + strand, within fumBat 1078.636 kb on + strand, within fumBat 1078.636 kb on + strand, within fumBat 1078.637 kb on - strand, within fumBat 1078.637 kb on - strand, within fumBat 1078.638 kb on + strand, within fumBat 1078.664 kb on + strand, within fumBat 1078.664 kb on + strand, within fumBat 1078.685 kb on - strand, within fumBat 1078.685 kb on - strand, within fumBat 1078.753 kb on + strand, within fumBat 1078.754 kb on - strand, within fumBat 1078.853 kb on - strand, within fumBat 1078.857 kb on + strand, within fumBat 1078.857 kb on + strand, within fumBat 1078.858 kb on - strand, within fumBat 1078.900 kb on - strand, within fumBat 1078.900 kb on - strand, within fumBat 1078.923 kb on + strand, within fumBat 1078.927 kb on + strand, within fumBat 1078.930 kb on + strand, within fumB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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1,076,231 + ydgA HEPCGN_06010 0.69 +0.9
1,076,270 + ydgA HEPCGN_06010 0.72 +0.2
1,076,271 - ydgA HEPCGN_06010 0.72 +0.0
1,076,652 + +2.1
1,076,693 + -1.1
1,076,749 + +0.9
1,076,751 + +0.1
1,076,752 - +2.3
1,076,752 - +0.2
1,076,893 - +1.2
1,076,986 - manA HEPCGN_06015 0.16 -0.5
1,077,127 - manA HEPCGN_06015 0.28 -0.3
1,077,600 - manA HEPCGN_06015 0.68 +1.0
1,077,600 - manA HEPCGN_06015 0.68 -0.9
1,077,600 - manA HEPCGN_06015 0.68 -1.2
1,077,909 - -0.6
1,077,944 + +2.6
1,077,992 - +0.2
1,078,016 - +0.1
1,078,228 - -0.9
1,078,234 + +0.2
1,078,238 - +0.3
1,078,265 + +0.0
1,078,313 + +0.6
1,078,314 - +0.8
1,078,422 + fumB HEPCGN_06020 0.15 +0.4
1,078,426 + fumB HEPCGN_06020 0.15 -1.0
1,078,427 - fumB HEPCGN_06020 0.15 -0.2
1,078,509 + fumB HEPCGN_06020 0.20 +0.0
1,078,582 - fumB HEPCGN_06020 0.25 +1.7
1,078,589 - fumB HEPCGN_06020 0.25 +1.9
1,078,589 - fumB HEPCGN_06020 0.25 -0.3
1,078,589 - fumB HEPCGN_06020 0.25 +0.5
1,078,589 - fumB HEPCGN_06020 0.25 -0.7
1,078,589 - fumB HEPCGN_06020 0.25 +0.2
1,078,636 + fumB HEPCGN_06020 0.28 +0.3
1,078,636 + fumB HEPCGN_06020 0.28 +0.7
1,078,636 + fumB HEPCGN_06020 0.28 -0.6
1,078,636 + fumB HEPCGN_06020 0.28 +0.1
1,078,636 + fumB HEPCGN_06020 0.28 -0.6
1,078,637 - fumB HEPCGN_06020 0.28 -1.3
1,078,637 - fumB HEPCGN_06020 0.28 +0.4
1,078,638 + fumB HEPCGN_06020 0.28 -0.5
1,078,664 + fumB HEPCGN_06020 0.30 +1.1
1,078,664 + fumB HEPCGN_06020 0.30 +0.3
1,078,685 - fumB HEPCGN_06020 0.31 +3.2
1,078,685 - fumB HEPCGN_06020 0.31 -2.7
1,078,753 + fumB HEPCGN_06020 0.35 -0.3
1,078,754 - fumB HEPCGN_06020 0.35 +2.2
1,078,853 - fumB HEPCGN_06020 0.41 -0.1
1,078,857 + fumB HEPCGN_06020 0.41 -2.1
1,078,857 + fumB HEPCGN_06020 0.41 +1.0
1,078,858 - fumB HEPCGN_06020 0.42 -0.1
1,078,900 - fumB HEPCGN_06020 0.44 -1.4
1,078,900 - fumB HEPCGN_06020 0.44 -0.1
1,078,923 + fumB HEPCGN_06020 0.45 +0.3
1,078,927 + fumB HEPCGN_06020 0.46 -0.5
1,078,930 + fumB HEPCGN_06020 0.46 -0.7

Or see this region's nucleotide sequence