Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menI and ydiH are separated by 99 nucleotides ydiH and sufA are separated by 548 nucleotides sufA and sufB are separated by 8 nucleotides
HEPCGN_05620: menI - 1,4-dihydroxy-2-naphthoyl-CoA hydrolase, at 999,659 to 1,000,069
menI
HEPCGN_05625: ydiH - Uncharacterized protein YdiH, at 1,000,169 to 1,000,357
ydiH
HEPCGN_05635: sufA - Fe-S cluster assembly scaffold SufA, at 1,000,906 to 1,001,274
sufA
HEPCGN_05640: sufB - Fe-S cluster assembly protein SufB, at 1,001,283 to 1,002,770
sufB
Position (kb) 1000
1001
1002 Strain fitness (log2 ratio) -2
-1
0
1
2 at 999.961 kb on - strand, within menI at 1000.096 kb on + strand at 1000.097 kb on - strand at 1000.133 kb on + strand at 1000.134 kb on - strand at 1000.135 kb on + strand at 1000.135 kb on + strand at 1000.135 kb on + strand at 1000.168 kb on + strand at 1000.168 kb on + strand at 1000.168 kb on + strand at 1000.168 kb on + strand at 1000.169 kb on - strand at 1000.247 kb on + strand, within ydiH at 1000.295 kb on - strand, within ydiH at 1000.329 kb on - strand, within ydiH at 1000.398 kb on + strand at 1000.428 kb on - strand at 1000.428 kb on - strand at 1000.431 kb on - strand at 1000.507 kb on - strand at 1000.507 kb on - strand at 1000.507 kb on - strand at 1000.514 kb on + strand at 1000.515 kb on - strand at 1000.539 kb on - strand at 1000.578 kb on - strand at 1000.655 kb on - strand at 1000.716 kb on + strand at 1000.780 kb on + strand at 1000.781 kb on - strand at 1000.781 kb on - strand at 1000.873 kb on + strand at 1000.876 kb on - strand at 1000.930 kb on - strand at 1001.069 kb on - strand, within sufA at 1001.280 kb on - strand at 1001.282 kb on + strand at 1001.283 kb on - strand at 1001.283 kb on - strand at 1001.343 kb on + strand at 1001.432 kb on + strand, within sufB at 1001.550 kb on + strand, within sufB at 1001.550 kb on + strand, within sufB at 1001.553 kb on + strand, within sufB at 1001.554 kb on - strand, within sufB at 1001.643 kb on - strand, within sufB at 1001.876 kb on + strand, within sufB at 1001.910 kb on + strand, within sufB at 1001.910 kb on + strand, within sufB at 1001.910 kb on + strand, within sufB at 1002.021 kb on + strand, within sufB at 1002.021 kb on + strand, within sufB at 1002.021 kb on + strand, within sufB at 1002.022 kb on - strand, within sufB at 1002.022 kb on - strand, within sufB at 1002.066 kb on + strand, within sufB at 1002.067 kb on - strand, within sufB at 1002.267 kb on + strand, within sufB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 999,961 - menI HEPCGN_05620 0.73 +1.9 1,000,096 + -0.1 1,000,097 - -0.3 1,000,133 + +1.8 1,000,134 - -1.3 1,000,135 + +0.6 1,000,135 + -0.0 1,000,135 + +0.1 1,000,168 + -0.7 1,000,168 + -0.4 1,000,168 + +0.3 1,000,168 + +0.4 1,000,169 - +1.1 1,000,247 + ydiH HEPCGN_05625 0.41 +1.9 1,000,295 - ydiH HEPCGN_05625 0.67 -0.2 1,000,329 - ydiH HEPCGN_05625 0.85 +0.9 1,000,398 + +0.1 1,000,428 - +0.2 1,000,428 - -0.5 1,000,431 - -1.8 1,000,507 - -0.5 1,000,507 - +1.4 1,000,507 - -1.0 1,000,514 + -0.1 1,000,515 - +0.6 1,000,539 - -0.5 1,000,578 - +1.0 1,000,655 - -0.3 1,000,716 + +1.5 1,000,780 + -2.3 1,000,781 - -0.1 1,000,781 - +1.1 1,000,873 + -1.8 1,000,876 - +0.3 1,000,930 - -0.3 1,001,069 - sufA HEPCGN_05635 0.44 -0.8 1,001,280 - -2.6 1,001,282 + +0.0 1,001,283 - +0.5 1,001,283 - +1.3 1,001,343 + -0.1 1,001,432 + sufB HEPCGN_05640 0.10 +0.2 1,001,550 + sufB HEPCGN_05640 0.18 -0.8 1,001,550 + sufB HEPCGN_05640 0.18 +0.1 1,001,553 + sufB HEPCGN_05640 0.18 -0.6 1,001,554 - sufB HEPCGN_05640 0.18 +2.2 1,001,643 - sufB HEPCGN_05640 0.24 +1.0 1,001,876 + sufB HEPCGN_05640 0.40 -1.2 1,001,910 + sufB HEPCGN_05640 0.42 +0.2 1,001,910 + sufB HEPCGN_05640 0.42 -0.8 1,001,910 + sufB HEPCGN_05640 0.42 -0.8 1,002,021 + sufB HEPCGN_05640 0.50 -1.3 1,002,021 + sufB HEPCGN_05640 0.50 -0.0 1,002,021 + sufB HEPCGN_05640 0.50 +0.4 1,002,022 - sufB HEPCGN_05640 0.50 +0.5 1,002,022 - sufB HEPCGN_05640 0.50 -0.0 1,002,066 + sufB HEPCGN_05640 0.53 -1.2 1,002,067 - sufB HEPCGN_05640 0.53 +0.1 1,002,267 + sufB HEPCGN_05640 0.66 -1.2
Or see this region's nucleotide sequence