Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hcxB and HEPCGN_01780 are separated by 66 nucleotides HEPCGN_01780 and ybiJ are separated by 13 nucleotides ybiJ and ybiI are separated by 264 nucleotides ybiI and HEPCGN_01795 are separated by 73 nucleotides
HEPCGN_01775: hcxB - hydroxycarboxylate dehydrogenase HcXB, at 280,112 to 281,197
hcxB
HEPCGN_01780: HEPCGN_01780 - Protein YbiE, at 281,264 to 281,323
_01780
HEPCGN_01785: ybiJ - DUF1471 domain-containing protein, at 281,337 to 281,597
ybiJ
HEPCGN_01790: ybiI - C4-type zinc finger protein YbiI, at 281,862 to 282,128
ybiI
HEPCGN_01795: HEPCGN_01795 - PKHD-type hydroxylase YbiX, at 282,202 to 282,876
_01795
Position (kb) 281
282 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 280.459 kb on + strand, within hcxB at 280.459 kb on + strand, within hcxB at 280.460 kb on - strand, within hcxB at 280.460 kb on - strand, within hcxB at 280.525 kb on + strand, within hcxB at 280.631 kb on - strand, within hcxB at 280.813 kb on + strand, within hcxB at 280.821 kb on - strand, within hcxB at 280.973 kb on - strand, within hcxB at 281.029 kb on + strand, within hcxB at 281.236 kb on - strand at 281.236 kb on - strand at 281.240 kb on + strand at 281.241 kb on - strand at 281.241 kb on - strand at 281.241 kb on - strand at 281.257 kb on - strand at 281.264 kb on - strand at 281.293 kb on + strand, within HEPCGN_01780 at 281.327 kb on - strand at 281.339 kb on - strand at 281.369 kb on + strand, within ybiJ at 281.369 kb on + strand, within ybiJ at 281.568 kb on - strand, within ybiJ at 281.582 kb on + strand at 281.583 kb on - strand at 281.588 kb on + strand at 281.589 kb on - strand at 281.598 kb on - strand at 281.627 kb on - strand at 281.678 kb on + strand at 281.723 kb on + strand at 281.733 kb on - strand at 281.760 kb on + strand at 281.833 kb on - strand at 281.843 kb on + strand at 281.844 kb on - strand at 281.844 kb on - strand at 281.844 kb on - strand at 281.899 kb on + strand, within ybiI at 281.906 kb on + strand, within ybiI at 282.019 kb on + strand, within ybiI at 282.077 kb on + strand, within ybiI at 282.160 kb on + strand at 282.161 kb on - strand at 282.161 kb on - strand at 282.170 kb on + strand at 282.170 kb on + strand at 282.171 kb on - strand at 282.173 kb on + strand at 282.174 kb on - strand at 282.175 kb on + strand at 282.175 kb on + strand at 282.175 kb on + strand at 282.176 kb on - strand at 282.176 kb on - strand at 282.176 kb on - strand at 282.364 kb on - strand, within HEPCGN_01795 at 282.426 kb on - strand, within HEPCGN_01795 at 282.426 kb on - strand, within HEPCGN_01795 at 282.481 kb on - strand, within HEPCGN_01795 at 282.546 kb on + strand, within HEPCGN_01795
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 280,459 + hcxB HEPCGN_01775 0.32 -0.3 280,459 + hcxB HEPCGN_01775 0.32 -0.1 280,460 - hcxB HEPCGN_01775 0.32 -0.3 280,460 - hcxB HEPCGN_01775 0.32 +0.1 280,525 + hcxB HEPCGN_01775 0.38 -0.2 280,631 - hcxB HEPCGN_01775 0.48 +0.2 280,813 + hcxB HEPCGN_01775 0.65 -0.7 280,821 - hcxB HEPCGN_01775 0.65 +1.1 280,973 - hcxB HEPCGN_01775 0.79 -1.0 281,029 + hcxB HEPCGN_01775 0.84 -1.1 281,236 - -3.3 281,236 - -0.4 281,240 + -0.1 281,241 - -0.5 281,241 - -0.7 281,241 - -0.9 281,257 - +2.1 281,264 - +0.1 281,293 + HEPCGN_01780 0.48 +0.9 281,327 - +0.5 281,339 - +0.2 281,369 + ybiJ HEPCGN_01785 0.12 -0.5 281,369 + ybiJ HEPCGN_01785 0.12 -1.2 281,568 - ybiJ HEPCGN_01785 0.89 -1.5 281,582 + +0.5 281,583 - -2.5 281,588 + +1.9 281,589 - +0.0 281,598 - +1.5 281,627 - +0.3 281,678 + +0.1 281,723 + +0.8 281,733 - +1.9 281,760 + -0.3 281,833 - -0.2 281,843 + +0.2 281,844 - -0.3 281,844 - -0.7 281,844 - -0.2 281,899 + ybiI HEPCGN_01790 0.14 -0.8 281,906 + ybiI HEPCGN_01790 0.16 -0.2 282,019 + ybiI HEPCGN_01790 0.59 -1.2 282,077 + ybiI HEPCGN_01790 0.81 +1.9 282,160 + -0.6 282,161 - -0.1 282,161 - -0.2 282,170 + -0.9 282,170 + +0.7 282,171 - -0.9 282,173 + +0.5 282,174 - -0.3 282,175 + +0.1 282,175 + +0.8 282,175 + +0.8 282,176 - -0.2 282,176 - -0.9 282,176 - -0.6 282,364 - HEPCGN_01795 0.24 +1.0 282,426 - HEPCGN_01795 0.33 -1.4 282,426 - HEPCGN_01795 0.33 +0.3 282,481 - HEPCGN_01795 0.41 -3.4 282,546 + HEPCGN_01795 0.51 +0.5
Or see this region's nucleotide sequence