Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybfF and seqA are separated by 184 nucleotides seqA and pgm are separated by 25 nucleotides
HEPCGN_01115: ybfF - esterase, at 146,616 to 147,380
ybfF
HEPCGN_01120: seqA - replication initiation negative regulator SeqA, at 147,565 to 148,110
seqA
HEPCGN_01125: pgm - phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent), at 148,136 to 149,776
pgm
Position (kb) 147
148
149 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 146.604 kb on + strand at 146.731 kb on - strand, within ybfF at 146.922 kb on - strand, within ybfF at 147.018 kb on + strand, within ybfF at 147.018 kb on + strand, within ybfF at 147.079 kb on - strand, within ybfF at 147.087 kb on - strand, within ybfF at 147.125 kb on + strand, within ybfF at 147.126 kb on - strand, within ybfF at 147.191 kb on - strand, within ybfF at 147.234 kb on - strand, within ybfF at 147.248 kb on + strand, within ybfF at 147.248 kb on + strand, within ybfF at 147.248 kb on + strand, within ybfF at 147.248 kb on + strand, within ybfF at 147.248 kb on + strand, within ybfF at 147.249 kb on - strand, within ybfF at 147.279 kb on + strand, within ybfF at 147.336 kb on - strand at 147.416 kb on + strand at 147.416 kb on + strand at 147.417 kb on - strand at 147.419 kb on + strand at 147.419 kb on + strand at 147.419 kb on + strand at 147.419 kb on + strand at 147.420 kb on - strand at 147.420 kb on - strand at 147.453 kb on + strand at 147.454 kb on - strand at 147.511 kb on + strand at 147.518 kb on - strand at 147.518 kb on - strand at 147.520 kb on - strand at 147.520 kb on - strand at 147.543 kb on + strand at 147.595 kb on + strand at 147.601 kb on + strand at 147.601 kb on + strand at 147.601 kb on + strand at 147.618 kb on + strand at 147.657 kb on + strand, within seqA at 147.805 kb on + strand, within seqA at 147.853 kb on - strand, within seqA at 147.859 kb on + strand, within seqA at 147.859 kb on + strand, within seqA at 147.860 kb on - strand, within seqA at 147.912 kb on + strand, within seqA at 147.991 kb on - strand, within seqA at 148.105 kb on - strand at 148.211 kb on + strand at 148.211 kb on + strand at 148.214 kb on + strand at 148.214 kb on + strand at 148.215 kb on - strand at 148.218 kb on + strand at 148.218 kb on + strand at 148.219 kb on - strand at 148.379 kb on + strand, within pgm at 148.380 kb on - strand, within pgm at 148.380 kb on - strand, within pgm at 148.380 kb on - strand, within pgm at 148.380 kb on - strand, within pgm at 148.564 kb on + strand, within pgm at 148.564 kb on + strand, within pgm at 148.565 kb on - strand, within pgm at 148.863 kb on - strand, within pgm at 148.886 kb on + strand, within pgm
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 146,604 + +1.5 146,731 - ybfF HEPCGN_01115 0.15 -0.4 146,922 - ybfF HEPCGN_01115 0.40 +0.2 147,018 + ybfF HEPCGN_01115 0.53 +0.3 147,018 + ybfF HEPCGN_01115 0.53 -0.6 147,079 - ybfF HEPCGN_01115 0.61 +0.3 147,087 - ybfF HEPCGN_01115 0.62 -2.1 147,125 + ybfF HEPCGN_01115 0.67 -0.8 147,126 - ybfF HEPCGN_01115 0.67 +0.0 147,191 - ybfF HEPCGN_01115 0.75 +0.4 147,234 - ybfF HEPCGN_01115 0.81 +0.8 147,248 + ybfF HEPCGN_01115 0.83 -2.5 147,248 + ybfF HEPCGN_01115 0.83 -0.7 147,248 + ybfF HEPCGN_01115 0.83 +0.4 147,248 + ybfF HEPCGN_01115 0.83 -1.7 147,248 + ybfF HEPCGN_01115 0.83 +0.2 147,249 - ybfF HEPCGN_01115 0.83 -1.8 147,279 + ybfF HEPCGN_01115 0.87 -0.4 147,336 - -0.2 147,416 + +0.2 147,416 + -0.6 147,417 - -1.0 147,419 + -1.1 147,419 + -1.0 147,419 + -0.0 147,419 + -2.0 147,420 - +0.4 147,420 - +0.8 147,453 + +0.4 147,454 - +1.1 147,511 + -1.0 147,518 - +0.8 147,518 - -0.9 147,520 - -0.9 147,520 - +3.0 147,543 + +0.3 147,595 + -2.6 147,601 + +0.8 147,601 + +1.4 147,601 + +1.4 147,618 + -0.2 147,657 + seqA HEPCGN_01120 0.17 +0.6 147,805 + seqA HEPCGN_01120 0.44 -0.0 147,853 - seqA HEPCGN_01120 0.53 +0.6 147,859 + seqA HEPCGN_01120 0.54 -0.5 147,859 + seqA HEPCGN_01120 0.54 +1.0 147,860 - seqA HEPCGN_01120 0.54 -1.1 147,912 + seqA HEPCGN_01120 0.64 +2.0 147,991 - seqA HEPCGN_01120 0.78 -1.3 148,105 - +2.0 148,211 + -0.6 148,211 + -0.3 148,214 + -0.7 148,214 + -0.4 148,215 - -0.2 148,218 + -0.4 148,218 + +0.3 148,219 - +2.4 148,379 + pgm HEPCGN_01125 0.15 +0.3 148,380 - pgm HEPCGN_01125 0.15 +0.3 148,380 - pgm HEPCGN_01125 0.15 +1.0 148,380 - pgm HEPCGN_01125 0.15 -0.2 148,380 - pgm HEPCGN_01125 0.15 +0.7 148,564 + pgm HEPCGN_01125 0.26 +0.4 148,564 + pgm HEPCGN_01125 0.26 +0.1 148,565 - pgm HEPCGN_01125 0.26 +0.9 148,863 - pgm HEPCGN_01125 0.44 +0.5 148,886 + pgm HEPCGN_01125 0.46 +0.3
Or see this region's nucleotide sequence