Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ortT and HEPCGN_26265 are separated by 85 nucleotides HEPCGN_26265 and ydcZ are separated by 0 nucleotides ydcZ and mddA overlap by 4 nucleotides mddA and curA are separated by 180 nucleotides
HEPCGN_26260: ortT - orphan toxin OrtT, at 3,998,947 to 3,999,120
ortT
HEPCGN_26265: HEPCGN_26265 - DUF2526 domain-containing protein, at 3,999,206 to 3,999,439
_26265
HEPCGN_26270: ydcZ - Putative inner membrane exporter, YdcZ, at 3,999,440 to 3,999,889
ydcZ
HEPCGN_26275: mddA - L-methionine sulfoximine/L-methionine sulfone acetyltransferase, at 3,999,886 to 4,000,404
mddA
HEPCGN_26280: curA - NADPH-dependent curcumin/dihydrocurcumin reductase, at 4,000,585 to 4,001,622
curA
Position (kb) 3999
4000
4001 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3998.923 kb on - strand at 3999.050 kb on + strand, within ortT at 3999.157 kb on - strand at 3999.158 kb on + strand at 3999.159 kb on - strand at 3999.205 kb on + strand at 3999.329 kb on + strand, within HEPCGN_26265 at 3999.332 kb on - strand, within HEPCGN_26265 at 3999.382 kb on + strand, within HEPCGN_26265 at 3999.414 kb on + strand, within HEPCGN_26265 at 3999.518 kb on + strand, within ydcZ at 3999.593 kb on - strand, within ydcZ at 3999.593 kb on - strand, within ydcZ at 3999.593 kb on - strand, within ydcZ at 3999.665 kb on + strand, within ydcZ at 3999.665 kb on + strand, within ydcZ at 3999.666 kb on - strand, within ydcZ at 3999.666 kb on - strand, within ydcZ at 3999.667 kb on + strand, within ydcZ at 3999.668 kb on - strand, within ydcZ at 3999.668 kb on - strand, within ydcZ at 3999.724 kb on + strand, within ydcZ at 3999.725 kb on - strand, within ydcZ at 3999.732 kb on - strand, within ydcZ at 3999.732 kb on - strand, within ydcZ at 3999.934 kb on - strand at 3999.934 kb on - strand at 3999.964 kb on - strand, within mddA at 4000.102 kb on + strand, within mddA at 4000.222 kb on + strand, within mddA at 4000.294 kb on - strand, within mddA at 4000.422 kb on + strand at 4000.443 kb on + strand at 4000.444 kb on - strand at 4000.549 kb on - strand at 4000.710 kb on - strand, within curA at 4000.710 kb on - strand, within curA at 4000.723 kb on + strand, within curA at 4000.741 kb on + strand, within curA at 4000.741 kb on + strand, within curA at 4000.742 kb on - strand, within curA at 4000.743 kb on + strand, within curA at 4000.743 kb on + strand, within curA at 4000.744 kb on - strand, within curA at 4000.756 kb on - strand, within curA at 4000.897 kb on + strand, within curA at 4000.904 kb on + strand, within curA at 4000.905 kb on - strand, within curA at 4000.996 kb on + strand, within curA at 4000.996 kb on + strand, within curA at 4000.997 kb on - strand, within curA at 4001.048 kb on + strand, within curA at 4001.238 kb on + strand, within curA at 4001.245 kb on - strand, within curA at 4001.248 kb on + strand, within curA at 4001.249 kb on - strand, within curA at 4001.273 kb on + strand, within curA at 4001.301 kb on + strand, within curA at 4001.301 kb on + strand, within curA at 4001.301 kb on + strand, within curA at 4001.302 kb on - strand, within curA at 4001.302 kb on - strand, within curA at 4001.342 kb on - strand, within curA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 3,998,923 - +0.0 3,999,050 + ortT HEPCGN_26260 0.59 -0.9 3,999,157 - -1.6 3,999,158 + -0.7 3,999,159 - +0.5 3,999,205 + +0.1 3,999,329 + HEPCGN_26265 0.53 +0.4 3,999,332 - HEPCGN_26265 0.54 -1.0 3,999,382 + HEPCGN_26265 0.75 +0.2 3,999,414 + HEPCGN_26265 0.89 +0.3 3,999,518 + ydcZ HEPCGN_26270 0.17 +0.1 3,999,593 - ydcZ HEPCGN_26270 0.34 +0.0 3,999,593 - ydcZ HEPCGN_26270 0.34 +2.1 3,999,593 - ydcZ HEPCGN_26270 0.34 -2.5 3,999,665 + ydcZ HEPCGN_26270 0.50 +0.6 3,999,665 + ydcZ HEPCGN_26270 0.50 +0.5 3,999,666 - ydcZ HEPCGN_26270 0.50 +0.3 3,999,666 - ydcZ HEPCGN_26270 0.50 -1.5 3,999,667 + ydcZ HEPCGN_26270 0.50 +0.4 3,999,668 - ydcZ HEPCGN_26270 0.51 -0.3 3,999,668 - ydcZ HEPCGN_26270 0.51 +1.3 3,999,724 + ydcZ HEPCGN_26270 0.63 -2.5 3,999,725 - ydcZ HEPCGN_26270 0.63 -1.3 3,999,732 - ydcZ HEPCGN_26270 0.65 -0.3 3,999,732 - ydcZ HEPCGN_26270 0.65 -0.0 3,999,934 - +0.3 3,999,934 - +0.1 3,999,964 - mddA HEPCGN_26275 0.15 -1.4 4,000,102 + mddA HEPCGN_26275 0.42 -3.0 4,000,222 + mddA HEPCGN_26275 0.65 -2.0 4,000,294 - mddA HEPCGN_26275 0.79 -1.5 4,000,422 + +0.4 4,000,443 + +1.0 4,000,444 - -0.3 4,000,549 - -1.3 4,000,710 - curA HEPCGN_26280 0.12 +0.1 4,000,710 - curA HEPCGN_26280 0.12 +0.3 4,000,723 + curA HEPCGN_26280 0.13 +0.6 4,000,741 + curA HEPCGN_26280 0.15 -0.4 4,000,741 + curA HEPCGN_26280 0.15 +0.1 4,000,742 - curA HEPCGN_26280 0.15 +0.9 4,000,743 + curA HEPCGN_26280 0.15 -1.0 4,000,743 + curA HEPCGN_26280 0.15 -0.1 4,000,744 - curA HEPCGN_26280 0.15 +0.0 4,000,756 - curA HEPCGN_26280 0.16 +0.6 4,000,897 + curA HEPCGN_26280 0.30 +0.8 4,000,904 + curA HEPCGN_26280 0.31 +0.8 4,000,905 - curA HEPCGN_26280 0.31 +0.4 4,000,996 + curA HEPCGN_26280 0.40 -0.6 4,000,996 + curA HEPCGN_26280 0.40 +0.7 4,000,997 - curA HEPCGN_26280 0.40 -0.3 4,001,048 + curA HEPCGN_26280 0.45 +0.7 4,001,238 + curA HEPCGN_26280 0.63 +0.1 4,001,245 - curA HEPCGN_26280 0.64 -0.1 4,001,248 + curA HEPCGN_26280 0.64 -1.3 4,001,249 - curA HEPCGN_26280 0.64 -0.7 4,001,273 + curA HEPCGN_26280 0.66 +0.4 4,001,301 + curA HEPCGN_26280 0.69 -0.5 4,001,301 + curA HEPCGN_26280 0.69 +1.4 4,001,301 + curA HEPCGN_26280 0.69 -0.4 4,001,302 - curA HEPCGN_26280 0.69 +1.4 4,001,302 - curA HEPCGN_26280 0.69 +0.2 4,001,342 - curA HEPCGN_26280 0.73 +1.8
Or see this region's nucleotide sequence