Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mppA and ynaI are separated by 50 nucleotides ynaI and ynaJ are separated by 243 nucleotides ynaJ and uspE are separated by 49 nucleotides
HEPCGN_25905: mppA - murein tripeptide ABC transporter substrate-binding protein MppA, at 3,921,716 to 3,923,329
mppA
HEPCGN_25910: ynaI - low conductance mechanosensitive channel YnaI, at 3,923,380 to 3,924,411
ynaI
HEPCGN_25915: ynaJ - Uncharacterized protein YnaJ, at 3,924,655 to 3,924,912
ynaJ
HEPCGN_25920: uspE - universal stress protein UspE, at 3,924,962 to 3,925,912
uspE
Position (kb)
3923
3924
3925 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3922.456 kb on + strand, within mppA at 3922.478 kb on - strand, within mppA at 3922.532 kb on + strand, within mppA at 3922.593 kb on - strand, within mppA at 3922.738 kb on + strand, within mppA at 3922.946 kb on - strand, within mppA at 3923.008 kb on + strand, within mppA at 3923.034 kb on - strand, within mppA at 3923.036 kb on - strand, within mppA at 3923.061 kb on + strand, within mppA at 3923.172 kb on - strand at 3923.230 kb on + strand at 3923.297 kb on + strand at 3923.309 kb on - strand at 3923.312 kb on + strand at 3923.313 kb on - strand at 3923.404 kb on - strand at 3923.416 kb on - strand at 3923.417 kb on + strand at 3923.418 kb on - strand at 3923.462 kb on - strand at 3923.555 kb on - strand, within ynaI at 3923.653 kb on - strand, within ynaI at 3923.678 kb on + strand, within ynaI at 3923.683 kb on + strand, within ynaI at 3923.830 kb on - strand, within ynaI at 3923.891 kb on + strand, within ynaI at 3924.172 kb on - strand, within ynaI at 3924.220 kb on - strand, within ynaI at 3924.225 kb on - strand, within ynaI at 3924.225 kb on - strand, within ynaI at 3924.366 kb on + strand at 3924.526 kb on + strand at 3924.530 kb on + strand at 3924.535 kb on - strand at 3924.606 kb on + strand at 3924.606 kb on + strand at 3924.649 kb on + strand at 3924.649 kb on + strand at 3924.649 kb on + strand at 3924.649 kb on + strand at 3924.650 kb on - strand at 3924.661 kb on - strand at 3924.811 kb on + strand, within ynaJ at 3924.811 kb on + strand, within ynaJ at 3924.843 kb on + strand, within ynaJ at 3924.844 kb on - strand, within ynaJ at 3924.950 kb on - strand at 3925.003 kb on - strand at 3925.130 kb on + strand, within uspE at 3925.131 kb on - strand, within uspE at 3925.131 kb on - strand, within uspE at 3925.264 kb on - strand, within uspE at 3925.346 kb on - strand, within uspE
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 3,922,456 + mppA HEPCGN_25905 0.46 +1.7 3,922,478 - mppA HEPCGN_25905 0.47 -0.9 3,922,532 + mppA HEPCGN_25905 0.51 -0.1 3,922,593 - mppA HEPCGN_25905 0.54 -0.1 3,922,738 + mppA HEPCGN_25905 0.63 +1.1 3,922,946 - mppA HEPCGN_25905 0.76 -0.3 3,923,008 + mppA HEPCGN_25905 0.80 +0.0 3,923,034 - mppA HEPCGN_25905 0.82 -0.6 3,923,036 - mppA HEPCGN_25905 0.82 -0.9 3,923,061 + mppA HEPCGN_25905 0.83 -1.5 3,923,172 - -0.9 3,923,230 + -1.6 3,923,297 + +0.2 3,923,309 - -0.3 3,923,312 + -0.2 3,923,313 - +0.0 3,923,404 - +1.1 3,923,416 - -0.5 3,923,417 + +0.3 3,923,418 - -0.7 3,923,462 - +0.0 3,923,555 - ynaI HEPCGN_25910 0.17 -1.4 3,923,653 - ynaI HEPCGN_25910 0.26 +0.1 3,923,678 + ynaI HEPCGN_25910 0.29 +0.2 3,923,683 + ynaI HEPCGN_25910 0.29 -2.1 3,923,830 - ynaI HEPCGN_25910 0.44 -0.3 3,923,891 + ynaI HEPCGN_25910 0.50 +1.0 3,924,172 - ynaI HEPCGN_25910 0.77 -0.5 3,924,220 - ynaI HEPCGN_25910 0.81 -0.9 3,924,225 - ynaI HEPCGN_25910 0.82 -1.1 3,924,225 - ynaI HEPCGN_25910 0.82 -0.4 3,924,366 + +0.6 3,924,526 + +1.2 3,924,530 + -0.2 3,924,535 - -0.7 3,924,606 + +0.2 3,924,606 + -1.2 3,924,649 + -0.2 3,924,649 + -1.9 3,924,649 + +2.0 3,924,649 + -0.2 3,924,650 - +0.9 3,924,661 - +0.2 3,924,811 + ynaJ HEPCGN_25915 0.60 +0.3 3,924,811 + ynaJ HEPCGN_25915 0.60 -1.5 3,924,843 + ynaJ HEPCGN_25915 0.73 -0.2 3,924,844 - ynaJ HEPCGN_25915 0.73 -0.1 3,924,950 - +1.6 3,925,003 - +0.8 3,925,130 + uspE HEPCGN_25920 0.18 +1.7 3,925,131 - uspE HEPCGN_25920 0.18 -1.8 3,925,131 - uspE HEPCGN_25920 0.18 -2.0 3,925,264 - uspE HEPCGN_25920 0.32 -0.9 3,925,346 - uspE HEPCGN_25920 0.40 +0.3
Or see this region's nucleotide sequence