Strain Fitness in Escherichia coli ECOR38 around HEPCGN_20765

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntiscS and iscU are separated by 27 nucleotidesiscU and iscA are separated by 16 nucleotidesiscA and hscB are separated by 95 nucleotideshscB and hscA are separated by 2 nucleotides HEPCGN_20760: iscS - cysteine desulfurase, at 2,892,535 to 2,893,749 iscS HEPCGN_20765: iscU - Iron-sulfur cluster assembly scaffold protein IscU, at 2,893,777 to 2,894,163 iscU HEPCGN_20770: iscA - iron-sulfur cluster assembly protein IscA, at 2,894,180 to 2,894,503 iscA HEPCGN_20775: hscB - co-chaperone HscB, at 2,894,599 to 2,895,129 hscB HEPCGN_20780: hscA - Fe-S protein assembly chaperone HscA, at 2,895,132 to 2,896,982 hscA Position (kb) 2893 2894 2895Strain fitness (log2 ratio) -2 -1 0 1 2at 2893.929 kb on + strand, within iscUat 2893.951 kb on - strand, within iscUat 2894.221 kb on + strand, within iscAat 2894.297 kb on + strand, within iscAat 2894.298 kb on - strand, within iscAat 2894.401 kb on + strand, within iscAat 2894.418 kb on + strand, within iscAat 2894.431 kb on + strand, within iscAat 2894.599 kb on - strandat 2894.647 kb on - strandat 2894.851 kb on - strand, within hscBat 2895.005 kb on + strand, within hscB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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2,893,929 + iscU HEPCGN_20765 0.39 +0.1
2,893,951 - iscU HEPCGN_20765 0.45 -1.8
2,894,221 + iscA HEPCGN_20770 0.13 +0.1
2,894,297 + iscA HEPCGN_20770 0.36 -0.7
2,894,298 - iscA HEPCGN_20770 0.36 -0.3
2,894,401 + iscA HEPCGN_20770 0.68 +0.6
2,894,418 + iscA HEPCGN_20770 0.73 +1.3
2,894,431 + iscA HEPCGN_20770 0.77 +1.9
2,894,599 - -1.0
2,894,647 - +0.3
2,894,851 - hscB HEPCGN_20775 0.47 -1.4
2,895,005 + hscB HEPCGN_20775 0.76 +1.1

Or see this region's nucleotide sequence