Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt era and recO are separated by 11 nucleotides recO and pdxJ are separated by 11 nucleotides pdxJ and HEPCGN_20575 overlap by 1 nucleotides HEPCGN_20575 and shoB are separated by 421 nucleotides
HEPCGN_20560: era - GTPase Era, at 2,850,976 to 2,851,881
era
HEPCGN_20565: recO - DNA repair protein RecO, at 2,851,893 to 2,852,621
recO
HEPCGN_20570: pdxJ - pyridoxine 5'-phosphate synthase, at 2,852,633 to 2,853,364
pdxJ
HEPCGN_20575: HEPCGN_20575 - Holo-[acyl-carrier-protein] synthase, at 2,853,364 to 2,853,744
_20575
HEPCGN_20585: shoB - Small toxic protein ShoB, at 2,854,166 to 2,854,246
shoB
Position (kb)
2852
2853
2854 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2851.925 kb on + strand at 2851.926 kb on - strand at 2851.926 kb on - strand at 2852.024 kb on + strand, within recO at 2852.066 kb on - strand, within recO at 2852.066 kb on - strand, within recO at 2852.135 kb on - strand, within recO at 2852.141 kb on + strand, within recO at 2852.142 kb on - strand, within recO at 2852.163 kb on + strand, within recO at 2852.163 kb on + strand, within recO at 2852.164 kb on - strand, within recO at 2852.299 kb on + strand, within recO at 2852.365 kb on + strand, within recO at 2852.383 kb on - strand, within recO at 2852.432 kb on + strand, within recO at 2852.432 kb on + strand, within recO at 2852.455 kb on + strand, within recO at 2852.456 kb on - strand, within recO at 2852.464 kb on + strand, within recO at 2852.538 kb on + strand, within recO at 2852.613 kb on + strand at 2852.668 kb on + strand at 2852.769 kb on + strand, within pdxJ at 2852.934 kb on + strand, within pdxJ at 2853.221 kb on + strand, within pdxJ at 2853.238 kb on + strand, within pdxJ at 2853.238 kb on + strand, within pdxJ at 2853.238 kb on + strand, within pdxJ at 2853.286 kb on + strand, within pdxJ at 2853.966 kb on - strand at 2853.969 kb on + strand at 2854.011 kb on + strand at 2854.120 kb on + strand at 2854.121 kb on - strand at 2854.121 kb on - strand at 2854.121 kb on - strand at 2854.128 kb on - strand at 2854.190 kb on - strand, within shoB at 2854.301 kb on + strand at 2854.301 kb on + strand at 2854.308 kb on - strand at 2854.332 kb on + strand at 2854.335 kb on - strand at 2854.335 kb on - strand at 2854.345 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 2,851,925 + +0.9 2,851,926 - +1.8 2,851,926 - +0.5 2,852,024 + recO HEPCGN_20565 0.18 -0.0 2,852,066 - recO HEPCGN_20565 0.24 +0.4 2,852,066 - recO HEPCGN_20565 0.24 +0.9 2,852,135 - recO HEPCGN_20565 0.33 +1.3 2,852,141 + recO HEPCGN_20565 0.34 +0.5 2,852,142 - recO HEPCGN_20565 0.34 +0.9 2,852,163 + recO HEPCGN_20565 0.37 +1.1 2,852,163 + recO HEPCGN_20565 0.37 +1.8 2,852,164 - recO HEPCGN_20565 0.37 +1.6 2,852,299 + recO HEPCGN_20565 0.56 +1.4 2,852,365 + recO HEPCGN_20565 0.65 +1.0 2,852,383 - recO HEPCGN_20565 0.67 +1.3 2,852,432 + recO HEPCGN_20565 0.74 +1.7 2,852,432 + recO HEPCGN_20565 0.74 +1.2 2,852,455 + recO HEPCGN_20565 0.77 +1.9 2,852,456 - recO HEPCGN_20565 0.77 +1.3 2,852,464 + recO HEPCGN_20565 0.78 +0.3 2,852,538 + recO HEPCGN_20565 0.88 +1.7 2,852,613 + -0.9 2,852,668 + -1.8 2,852,769 + pdxJ HEPCGN_20570 0.19 +0.6 2,852,934 + pdxJ HEPCGN_20570 0.41 +0.6 2,853,221 + pdxJ HEPCGN_20570 0.80 -1.5 2,853,238 + pdxJ HEPCGN_20570 0.83 -3.3 2,853,238 + pdxJ HEPCGN_20570 0.83 +0.2 2,853,238 + pdxJ HEPCGN_20570 0.83 -0.9 2,853,286 + pdxJ HEPCGN_20570 0.89 -1.1 2,853,966 - +0.4 2,853,969 + +0.1 2,854,011 + +0.7 2,854,120 + +1.4 2,854,121 - +1.5 2,854,121 - +0.5 2,854,121 - -1.5 2,854,128 - +0.5 2,854,190 - shoB HEPCGN_20585 0.30 +0.2 2,854,301 + -2.1 2,854,301 + -1.7 2,854,308 - -1.5 2,854,332 + +0.4 2,854,335 - +1.1 2,854,335 - +0.4 2,854,345 - -0.7
Or see this region's nucleotide sequence