Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mltB and pncC are separated by 144 nucleotides pncC and recA are separated by 79 nucleotides recA and recX are separated by 68 nucleotides recX and alaS are separated by 128 nucleotides
HEPCGN_19890: mltB - lytic murein transglycosylase B, at 2,731,848 to 2,733,038
mltB
HEPCGN_19895: pncC - nicotinamide-nucleotide amidase, at 2,733,183 to 2,733,680
pncC
HEPCGN_19900: recA - recombinase RecA, at 2,733,760 to 2,734,821
recA
HEPCGN_19905: recX - recombination regulator RecX, at 2,734,890 to 2,735,390
recX
HEPCGN_19910: alaS - alanine--tRNA ligase, at 2,735,519 to 2,738,149
alaS
Position (kb)
2733
2734
2735 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2732.820 kb on + strand, within mltB at 2732.851 kb on + strand, within mltB at 2732.966 kb on + strand at 2732.967 kb on - strand at 2732.967 kb on - strand at 2733.036 kb on + strand at 2733.040 kb on + strand at 2733.040 kb on + strand at 2733.040 kb on + strand at 2733.041 kb on - strand at 2733.041 kb on - strand at 2733.144 kb on - strand at 2733.348 kb on + strand, within pncC at 2733.474 kb on + strand, within pncC at 2733.486 kb on - strand, within pncC at 2733.576 kb on - strand, within pncC at 2733.579 kb on - strand, within pncC at 2733.636 kb on + strand at 2733.637 kb on - strand at 2733.637 kb on - strand at 2733.637 kb on - strand at 2733.637 kb on - strand at 2733.637 kb on - strand at 2733.701 kb on + strand at 2733.720 kb on + strand at 2733.720 kb on + strand at 2733.955 kb on + strand, within recA at 2734.023 kb on + strand, within recA at 2734.069 kb on + strand, within recA at 2734.069 kb on + strand, within recA at 2734.069 kb on + strand, within recA at 2734.070 kb on - strand, within recA at 2734.270 kb on - strand, within recA at 2734.270 kb on - strand, within recA at 2734.414 kb on + strand, within recA at 2734.444 kb on - strand, within recA at 2734.574 kb on - strand, within recA at 2734.730 kb on + strand at 2734.778 kb on + strand at 2734.836 kb on - strand at 2734.871 kb on + strand at 2734.878 kb on + strand at 2734.884 kb on + strand at 2734.884 kb on + strand at 2734.884 kb on + strand at 2734.885 kb on - strand at 2734.921 kb on - strand at 2735.091 kb on + strand, within recX at 2735.091 kb on + strand, within recX at 2735.092 kb on - strand, within recX at 2735.366 kb on + strand at 2735.449 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 2,732,820 + mltB HEPCGN_19890 0.82 +0.6 2,732,851 + mltB HEPCGN_19890 0.84 -0.3 2,732,966 + -0.0 2,732,967 - +0.6 2,732,967 - +0.4 2,733,036 + -0.9 2,733,040 + +0.2 2,733,040 + -0.3 2,733,040 + -0.8 2,733,041 - -1.3 2,733,041 - +0.5 2,733,144 - +0.2 2,733,348 + pncC HEPCGN_19895 0.33 -0.6 2,733,474 + pncC HEPCGN_19895 0.58 -0.0 2,733,486 - pncC HEPCGN_19895 0.61 +0.5 2,733,576 - pncC HEPCGN_19895 0.79 +0.9 2,733,579 - pncC HEPCGN_19895 0.80 +0.8 2,733,636 + +2.9 2,733,637 - -0.9 2,733,637 - +0.2 2,733,637 - -1.4 2,733,637 - +0.3 2,733,637 - -2.2 2,733,701 + +0.4 2,733,720 + +0.6 2,733,720 + +0.4 2,733,955 + recA HEPCGN_19900 0.18 -1.6 2,734,023 + recA HEPCGN_19900 0.25 +2.9 2,734,069 + recA HEPCGN_19900 0.29 -1.1 2,734,069 + recA HEPCGN_19900 0.29 +0.4 2,734,069 + recA HEPCGN_19900 0.29 +0.7 2,734,070 - recA HEPCGN_19900 0.29 -1.4 2,734,270 - recA HEPCGN_19900 0.48 -0.9 2,734,270 - recA HEPCGN_19900 0.48 -0.1 2,734,414 + recA HEPCGN_19900 0.62 -0.8 2,734,444 - recA HEPCGN_19900 0.64 -0.5 2,734,574 - recA HEPCGN_19900 0.77 +0.4 2,734,730 + +1.1 2,734,778 + -0.1 2,734,836 - +0.2 2,734,871 + +0.2 2,734,878 + +0.4 2,734,884 + -0.6 2,734,884 + -1.1 2,734,884 + -0.8 2,734,885 - +0.7 2,734,921 - +0.4 2,735,091 + recX HEPCGN_19905 0.40 -0.0 2,735,091 + recX HEPCGN_19905 0.40 +0.2 2,735,092 - recX HEPCGN_19905 0.40 -0.1 2,735,366 + +0.5 2,735,449 + -2.3
Or see this region's nucleotide sequence