Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt relA and mazE are separated by 77 nucleotides mazE and mazF overlap by 1 nucleotides mazF and mazG are separated by 71 nucleotides
HEPCGN_19500: relA - GTP diphosphokinase, at 2,656,812 to 2,659,046
relA
HEPCGN_19505: mazE - type II toxin-antitoxin system antitoxin MazE, at 2,659,124 to 2,659,372
mazE
HEPCGN_19510: mazF - endoribonuclease MazF, at 2,659,372 to 2,659,707
mazF
HEPCGN_19515: mazG - nucleoside triphosphate pyrophosphohydrolase, at 2,659,779 to 2,660,570
mazG
Position (kb)
2659
2660 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2658.200 kb on + strand, within relA at 2658.412 kb on - strand, within relA at 2658.564 kb on + strand, within relA at 2658.608 kb on - strand, within relA at 2658.793 kb on - strand, within relA at 2658.793 kb on - strand, within relA at 2658.850 kb on + strand at 2658.850 kb on + strand at 2658.850 kb on + strand at 2658.857 kb on + strand at 2658.892 kb on + strand at 2658.893 kb on - strand at 2658.893 kb on - strand at 2658.893 kb on - strand at 2658.992 kb on + strand at 2659.045 kb on - strand at 2659.045 kb on - strand at 2659.074 kb on - strand at 2659.086 kb on - strand at 2659.091 kb on - strand at 2659.091 kb on - strand at 2659.094 kb on + strand at 2659.141 kb on - strand at 2659.180 kb on + strand, within mazE at 2659.180 kb on + strand, within mazE at 2659.208 kb on - strand, within mazE at 2659.248 kb on + strand, within mazE at 2659.282 kb on + strand, within mazE at 2659.282 kb on + strand, within mazE at 2659.358 kb on - strand at 2659.375 kb on + strand at 2659.376 kb on + strand at 2659.486 kb on + strand, within mazF at 2659.486 kb on + strand, within mazF at 2659.486 kb on + strand, within mazF at 2659.504 kb on - strand, within mazF at 2659.586 kb on + strand, within mazF at 2659.593 kb on - strand, within mazF at 2659.593 kb on - strand, within mazF at 2659.713 kb on - strand at 2659.899 kb on + strand, within mazG at 2659.899 kb on + strand, within mazG at 2659.899 kb on + strand, within mazG at 2659.983 kb on + strand, within mazG at 2660.028 kb on - strand, within mazG at 2660.102 kb on + strand, within mazG at 2660.181 kb on - strand, within mazG at 2660.272 kb on + strand, within mazG at 2660.272 kb on + strand, within mazG at 2660.311 kb on - strand, within mazG
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 2,658,200 + relA HEPCGN_19500 0.62 -0.5 2,658,412 - relA HEPCGN_19500 0.72 -0.8 2,658,564 + relA HEPCGN_19500 0.78 +0.6 2,658,608 - relA HEPCGN_19500 0.80 -0.6 2,658,793 - relA HEPCGN_19500 0.89 +0.5 2,658,793 - relA HEPCGN_19500 0.89 -2.3 2,658,850 + +0.5 2,658,850 + -0.8 2,658,850 + -0.6 2,658,857 + -0.2 2,658,892 + -0.4 2,658,893 - -0.3 2,658,893 - +0.4 2,658,893 - +0.3 2,658,992 + -0.3 2,659,045 - +0.0 2,659,045 - -0.3 2,659,074 - +1.4 2,659,086 - +0.2 2,659,091 - +0.7 2,659,091 - -2.3 2,659,094 + -0.9 2,659,141 - -0.8 2,659,180 + mazE HEPCGN_19505 0.22 -3.6 2,659,180 + mazE HEPCGN_19505 0.22 -4.2 2,659,208 - mazE HEPCGN_19505 0.34 -0.4 2,659,248 + mazE HEPCGN_19505 0.50 -0.4 2,659,282 + mazE HEPCGN_19505 0.63 -3.4 2,659,282 + mazE HEPCGN_19505 0.63 -1.7 2,659,358 - +1.2 2,659,375 + -1.9 2,659,376 + -0.1 2,659,486 + mazF HEPCGN_19510 0.34 -0.2 2,659,486 + mazF HEPCGN_19510 0.34 +1.2 2,659,486 + mazF HEPCGN_19510 0.34 -0.7 2,659,504 - mazF HEPCGN_19510 0.39 -0.4 2,659,586 + mazF HEPCGN_19510 0.64 -1.0 2,659,593 - mazF HEPCGN_19510 0.66 +0.9 2,659,593 - mazF HEPCGN_19510 0.66 -0.4 2,659,713 - -0.0 2,659,899 + mazG HEPCGN_19515 0.15 +0.8 2,659,899 + mazG HEPCGN_19515 0.15 -0.2 2,659,899 + mazG HEPCGN_19515 0.15 -0.3 2,659,983 + mazG HEPCGN_19515 0.26 +0.3 2,660,028 - mazG HEPCGN_19515 0.31 +0.5 2,660,102 + mazG HEPCGN_19515 0.41 +0.1 2,660,181 - mazG HEPCGN_19515 0.51 +0.1 2,660,272 + mazG HEPCGN_19515 0.62 +1.0 2,660,272 + mazG HEPCGN_19515 0.62 +1.4 2,660,311 - mazG HEPCGN_19515 0.67 -1.0
Or see this region's nucleotide sequence