Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhdJ and fis are separated by 85 nucleotides fis and dusB are separated by 25 nucleotides
HEPCGN_16495: yhdJ - adenine-specific DNA-methyltransferase, at 2,045,938 to 2,046,822
yhdJ
HEPCGN_16500: fis - DNA-binding transcriptional regulator Fis, at 2,046,908 to 2,047,204
fis
HEPCGN_16505: dusB - tRNA dihydrouridine synthase DusB, at 2,047,230 to 2,048,195
dusB
Position (kb)
2046
2047
2048 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2045.928 kb on + strand at 2045.972 kb on - strand at 2046.080 kb on + strand, within yhdJ at 2046.081 kb on - strand, within yhdJ at 2046.144 kb on + strand, within yhdJ at 2046.144 kb on + strand, within yhdJ at 2046.144 kb on + strand, within yhdJ at 2046.145 kb on - strand, within yhdJ at 2046.443 kb on + strand, within yhdJ at 2046.443 kb on + strand, within yhdJ at 2046.444 kb on - strand, within yhdJ at 2046.489 kb on - strand, within yhdJ at 2046.489 kb on - strand, within yhdJ at 2046.543 kb on + strand, within yhdJ at 2046.576 kb on - strand, within yhdJ at 2046.576 kb on - strand, within yhdJ at 2046.694 kb on - strand, within yhdJ at 2046.694 kb on - strand, within yhdJ at 2046.721 kb on + strand, within yhdJ at 2046.721 kb on + strand, within yhdJ at 2046.721 kb on + strand, within yhdJ at 2046.722 kb on - strand, within yhdJ at 2046.946 kb on + strand, within fis at 2046.947 kb on - strand, within fis at 2047.054 kb on - strand, within fis at 2047.093 kb on - strand, within fis at 2047.188 kb on - strand at 2047.205 kb on - strand at 2047.250 kb on - strand at 2047.340 kb on - strand, within dusB at 2047.356 kb on - strand, within dusB at 2047.356 kb on - strand, within dusB at 2047.388 kb on - strand, within dusB at 2047.478 kb on - strand, within dusB at 2047.478 kb on - strand, within dusB at 2047.478 kb on - strand, within dusB at 2047.527 kb on + strand, within dusB at 2047.553 kb on - strand, within dusB at 2047.629 kb on - strand, within dusB at 2047.648 kb on + strand, within dusB at 2047.749 kb on - strand, within dusB at 2047.940 kb on + strand, within dusB at 2047.940 kb on + strand, within dusB at 2047.943 kb on + strand, within dusB at 2048.108 kb on - strand at 2048.135 kb on + strand at 2048.136 kb on - strand at 2048.136 kb on - strand at 2048.179 kb on + strand at 2048.201 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 2,045,928 + -0.9 2,045,972 - -0.4 2,046,080 + yhdJ HEPCGN_16495 0.16 +0.4 2,046,081 - yhdJ HEPCGN_16495 0.16 +1.7 2,046,144 + yhdJ HEPCGN_16495 0.23 +1.6 2,046,144 + yhdJ HEPCGN_16495 0.23 +0.0 2,046,144 + yhdJ HEPCGN_16495 0.23 +0.4 2,046,145 - yhdJ HEPCGN_16495 0.23 +1.1 2,046,443 + yhdJ HEPCGN_16495 0.57 +0.8 2,046,443 + yhdJ HEPCGN_16495 0.57 +0.6 2,046,444 - yhdJ HEPCGN_16495 0.57 -0.6 2,046,489 - yhdJ HEPCGN_16495 0.62 +0.4 2,046,489 - yhdJ HEPCGN_16495 0.62 -0.9 2,046,543 + yhdJ HEPCGN_16495 0.68 -1.0 2,046,576 - yhdJ HEPCGN_16495 0.72 -1.1 2,046,576 - yhdJ HEPCGN_16495 0.72 -0.2 2,046,694 - yhdJ HEPCGN_16495 0.85 +0.4 2,046,694 - yhdJ HEPCGN_16495 0.85 +1.0 2,046,721 + yhdJ HEPCGN_16495 0.88 +0.5 2,046,721 + yhdJ HEPCGN_16495 0.88 -0.5 2,046,721 + yhdJ HEPCGN_16495 0.88 +1.3 2,046,722 - yhdJ HEPCGN_16495 0.89 -1.2 2,046,946 + fis HEPCGN_16500 0.13 -1.1 2,046,947 - fis HEPCGN_16500 0.13 -2.5 2,047,054 - fis HEPCGN_16500 0.49 +1.5 2,047,093 - fis HEPCGN_16500 0.62 +0.1 2,047,188 - +1.5 2,047,205 - +1.1 2,047,250 - +0.1 2,047,340 - dusB HEPCGN_16505 0.11 +1.2 2,047,356 - dusB HEPCGN_16505 0.13 +0.6 2,047,356 - dusB HEPCGN_16505 0.13 -2.8 2,047,388 - dusB HEPCGN_16505 0.16 +1.1 2,047,478 - dusB HEPCGN_16505 0.26 +1.0 2,047,478 - dusB HEPCGN_16505 0.26 +0.8 2,047,478 - dusB HEPCGN_16505 0.26 +1.0 2,047,527 + dusB HEPCGN_16505 0.31 +1.2 2,047,553 - dusB HEPCGN_16505 0.33 +0.1 2,047,629 - dusB HEPCGN_16505 0.41 +0.3 2,047,648 + dusB HEPCGN_16505 0.43 -0.9 2,047,749 - dusB HEPCGN_16505 0.54 +0.4 2,047,940 + dusB HEPCGN_16505 0.73 -1.5 2,047,940 + dusB HEPCGN_16505 0.73 -1.2 2,047,943 + dusB HEPCGN_16505 0.74 -1.2 2,048,108 - +0.3 2,048,135 + -0.8 2,048,136 - -0.6 2,048,136 - +1.1 2,048,179 + -0.6 2,048,201 - +0.7
Or see this region's nucleotide sequence