Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metB and metJ are separated by 276 nucleotides metJ and yiiX are separated by 59 nucleotides yiiX and rpmE are separated by 60 nucleotides
HEPCGN_12345: metB - cystathionine gamma-synthase, at 1,220,062 to 1,221,222
metB
HEPCGN_12350: metJ - met regulon transcriptional regulator MetJ, at 1,221,499 to 1,221,816
metJ
HEPCGN_12355: yiiX - YiiX family permuted papain-like enzyme, at 1,221,876 to 1,222,484
yiiX
HEPCGN_12360: rpmE - 50S ribosomal protein L31, at 1,222,545 to 1,222,757
rpmE
Position (kb)
1221
1222 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1220.507 kb on + strand, within metB at 1220.507 kb on + strand, within metB at 1220.634 kb on + strand, within metB at 1220.663 kb on - strand, within metB at 1220.663 kb on - strand, within metB at 1220.681 kb on - strand, within metB at 1220.693 kb on - strand, within metB at 1220.693 kb on - strand, within metB at 1220.782 kb on - strand, within metB at 1220.794 kb on + strand, within metB at 1221.004 kb on - strand, within metB at 1221.108 kb on + strand at 1221.124 kb on - strand at 1221.125 kb on + strand at 1221.126 kb on - strand at 1221.126 kb on - strand at 1221.165 kb on - strand at 1221.271 kb on - strand at 1221.271 kb on - strand at 1221.296 kb on - strand at 1221.346 kb on - strand at 1221.367 kb on + strand at 1221.387 kb on + strand at 1221.421 kb on + strand at 1221.421 kb on + strand at 1221.421 kb on + strand at 1221.422 kb on - strand at 1221.432 kb on - strand at 1221.489 kb on + strand at 1221.489 kb on + strand at 1221.490 kb on - strand at 1221.490 kb on - strand at 1221.493 kb on + strand at 1221.494 kb on - strand at 1221.494 kb on - strand at 1221.520 kb on + strand at 1221.520 kb on + strand at 1221.521 kb on - strand at 1221.521 kb on - strand at 1221.521 kb on - strand at 1221.523 kb on - strand at 1221.523 kb on - strand at 1221.532 kb on + strand, within metJ at 1221.592 kb on - strand, within metJ at 1221.859 kb on + strand at 1221.871 kb on + strand at 1221.872 kb on - strand at 1222.022 kb on + strand, within yiiX at 1222.022 kb on + strand, within yiiX at 1222.028 kb on + strand, within yiiX at 1222.029 kb on - strand, within yiiX at 1222.057 kb on - strand, within yiiX at 1222.086 kb on + strand, within yiiX at 1222.087 kb on - strand, within yiiX at 1222.131 kb on + strand, within yiiX at 1222.132 kb on - strand, within yiiX at 1222.132 kb on - strand, within yiiX at 1222.132 kb on - strand, within yiiX at 1222.218 kb on + strand, within yiiX at 1222.218 kb on + strand, within yiiX at 1222.219 kb on - strand, within yiiX at 1222.227 kb on - strand, within yiiX at 1222.281 kb on + strand, within yiiX at 1222.409 kb on - strand, within yiiX at 1222.485 kb on + strand at 1222.485 kb on + strand at 1222.547 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 1,220,507 + metB HEPCGN_12345 0.38 -0.6 1,220,507 + metB HEPCGN_12345 0.38 -0.4 1,220,634 + metB HEPCGN_12345 0.49 -0.7 1,220,663 - metB HEPCGN_12345 0.52 -0.3 1,220,663 - metB HEPCGN_12345 0.52 +0.8 1,220,681 - metB HEPCGN_12345 0.53 -0.5 1,220,693 - metB HEPCGN_12345 0.54 -0.5 1,220,693 - metB HEPCGN_12345 0.54 -0.5 1,220,782 - metB HEPCGN_12345 0.62 +0.2 1,220,794 + metB HEPCGN_12345 0.63 +0.1 1,221,004 - metB HEPCGN_12345 0.81 -0.5 1,221,108 + -0.2 1,221,124 - -1.0 1,221,125 + +0.3 1,221,126 - +0.2 1,221,126 - +0.8 1,221,165 - +1.3 1,221,271 - +0.4 1,221,271 - -1.5 1,221,296 - -0.6 1,221,346 - -0.3 1,221,367 + +0.9 1,221,387 + +2.3 1,221,421 + +0.0 1,221,421 + +0.6 1,221,421 + +1.1 1,221,422 - -0.5 1,221,432 - +2.0 1,221,489 + -0.3 1,221,489 + -2.6 1,221,490 - -1.2 1,221,490 - -1.2 1,221,493 + -1.1 1,221,494 - +0.4 1,221,494 - +0.0 1,221,520 + -2.5 1,221,520 + -0.2 1,221,521 - -0.4 1,221,521 - -1.2 1,221,521 - -1.4 1,221,523 - -0.6 1,221,523 - -1.6 1,221,532 + metJ HEPCGN_12350 0.10 -0.9 1,221,592 - metJ HEPCGN_12350 0.29 -0.8 1,221,859 + -1.2 1,221,871 + -1.1 1,221,872 - -1.9 1,222,022 + yiiX HEPCGN_12355 0.24 -0.6 1,222,022 + yiiX HEPCGN_12355 0.24 +0.2 1,222,028 + yiiX HEPCGN_12355 0.25 -0.8 1,222,029 - yiiX HEPCGN_12355 0.25 +0.8 1,222,057 - yiiX HEPCGN_12355 0.30 +1.0 1,222,086 + yiiX HEPCGN_12355 0.34 +1.2 1,222,087 - yiiX HEPCGN_12355 0.35 -1.2 1,222,131 + yiiX HEPCGN_12355 0.42 +0.4 1,222,132 - yiiX HEPCGN_12355 0.42 +0.0 1,222,132 - yiiX HEPCGN_12355 0.42 +0.6 1,222,132 - yiiX HEPCGN_12355 0.42 +0.4 1,222,218 + yiiX HEPCGN_12355 0.56 +0.4 1,222,218 + yiiX HEPCGN_12355 0.56 -0.6 1,222,219 - yiiX HEPCGN_12355 0.56 -1.7 1,222,227 - yiiX HEPCGN_12355 0.58 +0.6 1,222,281 + yiiX HEPCGN_12355 0.67 -0.7 1,222,409 - yiiX HEPCGN_12355 0.88 -1.1 1,222,485 + -0.2 1,222,485 + -0.3 1,222,547 - +0.3
Or see this region's nucleotide sequence