Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cyoE and HEPCGN_07000 are separated by 184 nucleotides HEPCGN_07000 and HEPCGN_07005 overlap by 17 nucleotides HEPCGN_07005 and HEPCGN_07010 are separated by 55 nucleotides
HEPCGN_06995: cyoE - heme o synthase, at 135,535 to 136,425
cyoE
HEPCGN_07000: HEPCGN_07000 - DUF1778 domain-containing protein, at 136,610 to 136,921
_07000
HEPCGN_07005: HEPCGN_07005 - N-acetyltransferase, at 136,905 to 137,447
_07005
HEPCGN_07010: HEPCGN_07010 - sel1 repeat family protein, at 137,503 to 138,438
_07010
Position (kb) 136
137
138 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 135.906 kb on + strand, within cyoE at 135.906 kb on + strand, within cyoE at 136.067 kb on - strand, within cyoE at 136.097 kb on - strand, within cyoE at 136.169 kb on - strand, within cyoE at 136.169 kb on - strand, within cyoE at 136.169 kb on - strand, within cyoE at 136.171 kb on - strand, within cyoE at 136.227 kb on + strand, within cyoE at 136.232 kb on - strand, within cyoE at 136.232 kb on - strand, within cyoE at 136.232 kb on - strand, within cyoE at 136.272 kb on + strand, within cyoE at 136.289 kb on - strand, within cyoE at 136.290 kb on + strand, within cyoE at 136.384 kb on - strand at 136.401 kb on + strand at 136.423 kb on + strand at 136.427 kb on - strand at 136.427 kb on - strand at 136.490 kb on + strand at 136.500 kb on - strand at 136.557 kb on + strand at 136.608 kb on + strand at 136.609 kb on - strand at 136.609 kb on - strand at 136.952 kb on + strand at 136.957 kb on - strand at 136.970 kb on + strand, within HEPCGN_07005 at 136.971 kb on - strand, within HEPCGN_07005 at 136.971 kb on - strand, within HEPCGN_07005 at 136.971 kb on - strand, within HEPCGN_07005 at 136.975 kb on - strand, within HEPCGN_07005 at 137.012 kb on + strand, within HEPCGN_07005 at 137.027 kb on - strand, within HEPCGN_07005 at 137.055 kb on - strand, within HEPCGN_07005 at 137.203 kb on + strand, within HEPCGN_07005 at 137.203 kb on + strand, within HEPCGN_07005 at 137.257 kb on + strand, within HEPCGN_07005 at 137.258 kb on - strand, within HEPCGN_07005 at 137.258 kb on - strand, within HEPCGN_07005 at 137.258 kb on - strand, within HEPCGN_07005 at 137.258 kb on - strand, within HEPCGN_07005 at 137.310 kb on - strand, within HEPCGN_07005 at 137.310 kb on - strand, within HEPCGN_07005 at 137.310 kb on - strand, within HEPCGN_07005 at 137.352 kb on + strand, within HEPCGN_07005 at 137.353 kb on - strand, within HEPCGN_07005 at 137.433 kb on - strand at 137.451 kb on - strand at 137.473 kb on - strand at 137.626 kb on - strand, within HEPCGN_07010 at 137.626 kb on - strand, within HEPCGN_07010 at 137.669 kb on + strand, within HEPCGN_07010 at 137.670 kb on - strand, within HEPCGN_07010 at 137.796 kb on + strand, within HEPCGN_07010 at 137.887 kb on + strand, within HEPCGN_07010 at 137.961 kb on - strand, within HEPCGN_07010 at 138.008 kb on - strand, within HEPCGN_07010 at 138.018 kb on - strand, within HEPCGN_07010 at 138.018 kb on - strand, within HEPCGN_07010 at 138.018 kb on - strand, within HEPCGN_07010 at 138.058 kb on + strand, within HEPCGN_07010 at 138.059 kb on - strand, within HEPCGN_07010 at 138.098 kb on + strand, within HEPCGN_07010 at 138.140 kb on + strand, within HEPCGN_07010 at 138.140 kb on + strand, within HEPCGN_07010 at 138.144 kb on + strand, within HEPCGN_07010 at 138.171 kb on + strand, within HEPCGN_07010 at 138.204 kb on + strand, within HEPCGN_07010 at 138.249 kb on - strand, within HEPCGN_07010 at 138.252 kb on - strand, within HEPCGN_07010 at 138.440 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 135,906 + cyoE HEPCGN_06995 0.42 +0.5 135,906 + cyoE HEPCGN_06995 0.42 -0.8 136,067 - cyoE HEPCGN_06995 0.60 -0.1 136,097 - cyoE HEPCGN_06995 0.63 +1.0 136,169 - cyoE HEPCGN_06995 0.71 -1.4 136,169 - cyoE HEPCGN_06995 0.71 -1.0 136,169 - cyoE HEPCGN_06995 0.71 +0.2 136,171 - cyoE HEPCGN_06995 0.71 +2.9 136,227 + cyoE HEPCGN_06995 0.78 -1.5 136,232 - cyoE HEPCGN_06995 0.78 -0.7 136,232 - cyoE HEPCGN_06995 0.78 -0.2 136,232 - cyoE HEPCGN_06995 0.78 -3.0 136,272 + cyoE HEPCGN_06995 0.83 -2.2 136,289 - cyoE HEPCGN_06995 0.85 -0.0 136,290 + cyoE HEPCGN_06995 0.85 -0.5 136,384 - +0.2 136,401 + +0.1 136,423 + -0.6 136,427 - +1.9 136,427 - +0.3 136,490 + -0.8 136,500 - -1.5 136,557 + -0.6 136,608 + -0.2 136,609 - -0.4 136,609 - -1.5 136,952 + -0.2 136,957 - -1.1 136,970 + HEPCGN_07005 0.12 -0.8 136,971 - HEPCGN_07005 0.12 +0.1 136,971 - HEPCGN_07005 0.12 -0.2 136,971 - HEPCGN_07005 0.12 -0.3 136,975 - HEPCGN_07005 0.13 -0.5 137,012 + HEPCGN_07005 0.20 -1.2 137,027 - HEPCGN_07005 0.22 +1.2 137,055 - HEPCGN_07005 0.28 -0.9 137,203 + HEPCGN_07005 0.55 -0.2 137,203 + HEPCGN_07005 0.55 -0.2 137,257 + HEPCGN_07005 0.65 -0.9 137,258 - HEPCGN_07005 0.65 -2.0 137,258 - HEPCGN_07005 0.65 +0.2 137,258 - HEPCGN_07005 0.65 -0.9 137,258 - HEPCGN_07005 0.65 -1.0 137,310 - HEPCGN_07005 0.75 +0.6 137,310 - HEPCGN_07005 0.75 -0.0 137,310 - HEPCGN_07005 0.75 +0.2 137,352 + HEPCGN_07005 0.82 +0.1 137,353 - HEPCGN_07005 0.83 -0.9 137,433 - +0.1 137,451 - -0.1 137,473 - +0.5 137,626 - HEPCGN_07010 0.13 +0.7 137,626 - HEPCGN_07010 0.13 -0.3 137,669 + HEPCGN_07010 0.18 -0.7 137,670 - HEPCGN_07010 0.18 +0.9 137,796 + HEPCGN_07010 0.31 +0.9 137,887 + HEPCGN_07010 0.41 -2.0 137,961 - HEPCGN_07010 0.49 -0.6 138,008 - HEPCGN_07010 0.54 -2.4 138,018 - HEPCGN_07010 0.55 -1.1 138,018 - HEPCGN_07010 0.55 +0.1 138,018 - HEPCGN_07010 0.55 -0.6 138,058 + HEPCGN_07010 0.59 +0.0 138,059 - HEPCGN_07010 0.59 -0.1 138,098 + HEPCGN_07010 0.64 +0.2 138,140 + HEPCGN_07010 0.68 -0.7 138,140 + HEPCGN_07010 0.68 -0.3 138,144 + HEPCGN_07010 0.68 +0.1 138,171 + HEPCGN_07010 0.71 +0.2 138,204 + HEPCGN_07010 0.75 +2.9 138,249 - HEPCGN_07010 0.80 +0.2 138,252 - HEPCGN_07010 0.80 -0.8 138,440 + +0.3
Or see this region's nucleotide sequence