Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt odhB and sucC are separated by 93 nucleotides sucC and sucD overlap by 1 nucleotides sucD and HEPCGN_01325 are separated by 98 nucleotides
HEPCGN_01310: odhB - 2-oxoglutarate dehydrogenase complex dihydrolipoyllysine-residue succinyltransferase, at 188,940 to 190,157
odhB
HEPCGN_01315: sucC - ADP-forming succinate--CoA ligase subunit beta, at 190,251 to 191,417
sucC
HEPCGN_01320: sucD - succinate--CoA ligase subunit alpha, at 191,417 to 192,286
sucD
HEPCGN_01325: HEPCGN_01325 - RNA-splicing ligase RtcB, at 192,385 to 193,782
_01325
Position (kb) 190
191
192 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 189.314 kb on + strand, within odhB at 189.335 kb on + strand, within odhB at 189.641 kb on + strand, within odhB at 189.641 kb on + strand, within odhB at 189.641 kb on + strand, within odhB at 189.726 kb on + strand, within odhB at 189.735 kb on + strand, within odhB at 189.851 kb on + strand, within odhB at 190.044 kb on + strand at 190.159 kb on - strand at 190.181 kb on + strand at 190.216 kb on - strand at 190.258 kb on + strand at 190.259 kb on - strand at 190.301 kb on - strand at 190.317 kb on + strand at 190.325 kb on + strand at 190.326 kb on - strand at 190.500 kb on + strand, within sucC at 190.501 kb on - strand, within sucC at 190.598 kb on + strand, within sucC at 190.599 kb on - strand, within sucC at 191.004 kb on + strand, within sucC at 191.004 kb on + strand, within sucC at 191.004 kb on + strand, within sucC at 191.005 kb on - strand, within sucC at 191.033 kb on + strand, within sucC at 191.303 kb on + strand at 191.303 kb on + strand at 191.304 kb on - strand at 191.351 kb on + strand at 191.415 kb on + strand at 191.416 kb on - strand at 191.434 kb on + strand at 191.435 kb on - strand at 191.450 kb on - strand at 191.630 kb on + strand, within sucD at 191.630 kb on + strand, within sucD at 191.630 kb on + strand, within sucD at 191.631 kb on - strand, within sucD at 191.631 kb on - strand, within sucD at 191.631 kb on - strand, within sucD at 191.671 kb on + strand, within sucD at 191.696 kb on - strand, within sucD at 191.728 kb on + strand, within sucD at 191.855 kb on - strand, within sucD at 191.891 kb on + strand, within sucD at 191.974 kb on + strand, within sucD at 192.114 kb on - strand, within sucD at 192.149 kb on - strand, within sucD at 192.324 kb on + strand at 192.370 kb on + strand at 192.370 kb on + strand at 192.371 kb on - strand at 192.371 kb on - strand at 192.371 kb on - strand at 192.371 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 189,314 + odhB HEPCGN_01310 0.31 +1.1 189,335 + odhB HEPCGN_01310 0.32 +5.9 189,641 + odhB HEPCGN_01310 0.58 +2.7 189,641 + odhB HEPCGN_01310 0.58 +0.4 189,641 + odhB HEPCGN_01310 0.58 +2.2 189,726 + odhB HEPCGN_01310 0.65 +0.2 189,735 + odhB HEPCGN_01310 0.65 -0.3 189,851 + odhB HEPCGN_01310 0.75 -0.3 190,044 + +0.5 190,159 - +0.3 190,181 + -0.2 190,216 - -1.8 190,258 + +0.5 190,259 - -0.5 190,301 - -0.6 190,317 + -1.6 190,325 + -0.3 190,326 - -1.6 190,500 + sucC HEPCGN_01315 0.21 -0.5 190,501 - sucC HEPCGN_01315 0.21 -0.7 190,598 + sucC HEPCGN_01315 0.30 +0.5 190,599 - sucC HEPCGN_01315 0.30 -0.3 191,004 + sucC HEPCGN_01315 0.65 -1.0 191,004 + sucC HEPCGN_01315 0.65 -1.4 191,004 + sucC HEPCGN_01315 0.65 -1.9 191,005 - sucC HEPCGN_01315 0.65 -2.2 191,033 + sucC HEPCGN_01315 0.67 +0.6 191,303 + -0.5 191,303 + +0.7 191,304 - +0.7 191,351 + -0.5 191,415 + +1.3 191,416 - -0.2 191,434 + -0.5 191,435 - -0.4 191,450 - -0.4 191,630 + sucD HEPCGN_01320 0.24 -0.6 191,630 + sucD HEPCGN_01320 0.24 +0.2 191,630 + sucD HEPCGN_01320 0.24 -0.8 191,631 - sucD HEPCGN_01320 0.25 -0.1 191,631 - sucD HEPCGN_01320 0.25 +0.1 191,631 - sucD HEPCGN_01320 0.25 -1.1 191,671 + sucD HEPCGN_01320 0.29 +0.3 191,696 - sucD HEPCGN_01320 0.32 +1.2 191,728 + sucD HEPCGN_01320 0.36 -0.7 191,855 - sucD HEPCGN_01320 0.50 -0.7 191,891 + sucD HEPCGN_01320 0.54 -1.1 191,974 + sucD HEPCGN_01320 0.64 -0.2 192,114 - sucD HEPCGN_01320 0.80 +0.5 192,149 - sucD HEPCGN_01320 0.84 -3.1 192,324 + +0.3 192,370 + -2.3 192,370 + +0.8 192,371 - -0.6 192,371 - -3.2 192,371 - +0.6 192,371 - -0.8
Or see this region's nucleotide sequence