Experiment: R2A motility; outer cut
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR88_RS00820 and ABZR88_RS00825 are separated by 370 nucleotides ABZR88_RS00825 and ABZR88_RS00830 are separated by 243 nucleotides ABZR88_RS00830 and ABZR88_RS00835 are separated by 178 nucleotides
ABZR88_RS00820: ABZR88_RS00820 - D-2-hydroxyacid dehydrogenase, at 158,563 to 159,525
_RS00820
ABZR88_RS00825: ABZR88_RS00825 - cystathionine gamma-synthase family protein, at 159,896 to 161,140
_RS00825
ABZR88_RS00830: ABZR88_RS00830 - hypothetical protein, at 161,384 to 161,791
_RS00830
ABZR88_RS00835: ABZR88_RS00835 - TIM-barrel domain-containing protein, at 161,970 to 164,360
_RS00835
Position (kb) 159
160
161
162 Strain fitness (log2 ratio) -2
-1
0
1 at 158.986 kb on - strand, within ABZR88_RS00820 at 159.231 kb on + strand, within ABZR88_RS00820 at 159.231 kb on + strand, within ABZR88_RS00820 at 159.546 kb on - strand at 159.554 kb on + strand at 159.655 kb on - strand at 159.793 kb on + strand at 160.040 kb on + strand, within ABZR88_RS00825 at 160.279 kb on + strand, within ABZR88_RS00825 at 160.575 kb on + strand, within ABZR88_RS00825 at 160.576 kb on - strand, within ABZR88_RS00825 at 160.637 kb on + strand, within ABZR88_RS00825 at 161.010 kb on - strand, within ABZR88_RS00825 at 161.280 kb on - strand at 161.281 kb on + strand at 161.281 kb on + strand at 161.360 kb on - strand at 161.421 kb on + strand at 161.426 kb on - strand, within ABZR88_RS00830 at 161.627 kb on + strand, within ABZR88_RS00830 at 161.691 kb on - strand, within ABZR88_RS00830 at 161.726 kb on + strand, within ABZR88_RS00830 at 161.881 kb on + strand at 161.945 kb on + strand at 162.006 kb on - strand at 162.064 kb on - strand at 162.066 kb on + strand at 162.103 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A motility; outer cut remove 158,986 - ABZR88_RS00820 0.44 -0.3 159,231 + ABZR88_RS00820 0.69 +0.1 159,231 + ABZR88_RS00820 0.69 +0.3 159,546 - -0.7 159,554 + -0.4 159,655 - -0.2 159,793 + +0.2 160,040 + ABZR88_RS00825 0.12 -1.3 160,279 + ABZR88_RS00825 0.31 -1.7 160,575 + ABZR88_RS00825 0.55 +0.3 160,576 - ABZR88_RS00825 0.55 -0.7 160,637 + ABZR88_RS00825 0.60 +1.3 161,010 - ABZR88_RS00825 0.89 -0.7 161,280 - -0.4 161,281 + -0.0 161,281 + -0.6 161,360 - +0.7 161,421 + -0.7 161,426 - ABZR88_RS00830 0.10 +0.2 161,627 + ABZR88_RS00830 0.60 -2.5 161,691 - ABZR88_RS00830 0.75 +0.6 161,726 + ABZR88_RS00830 0.84 -0.1 161,881 + -1.2 161,945 + -1.5 162,006 - -0.9 162,064 - +0.4 162,066 + +0.0 162,103 + -0.7
Or see this region's nucleotide sequence