Strain Fitness in Escherichia coli ECOR38 around HEPCGN_26325

Experiment: Bas45

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHEPCGN_26315 and tnp-IS629 are separated by 129 nucleotidestnp-IS629 and tnp-IS629 overlap by 1 nucleotidestnp-IS629 and HEPCGN_26330 overlap by 119 nucleotidesHEPCGN_26330 and pptA are separated by 304 nucleotidespptA and yddH are separated by 3 nucleotides HEPCGN_26315: HEPCGN_26315 - Linear primary-alkylsulfatase, at 4,009,569 to 4,011,152 _26315 HEPCGN_26320: tnp-IS629 - IS3 family IS629 transposase, at 4,011,282 to 4,011,608 tnp-IS629 HEPCGN_26325: tnp-IS629 - IS3 family IS629 transposase, at 4,011,608 to 4,012,495 tnp-IS629 HEPCGN_26330: HEPCGN_26330 - hypothetical protein, at 4,012,377 to 4,012,859 _26330 HEPCGN_26335: pptA - tautomerase PptA, at 4,013,164 to 4,013,391 pptA HEPCGN_26340: yddH - Uncharacterized protein YddH, at 4,013,395 to 4,013,964 yddH Position (kb) 4011 4012 4013Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4010.758 kb on + strand, within HEPCGN_26315at 4010.861 kb on + strand, within HEPCGN_26315at 4010.861 kb on + strand, within HEPCGN_26315at 4010.862 kb on - strand, within HEPCGN_26315at 4010.862 kb on - strand, within HEPCGN_26315at 4010.862 kb on - strand, within HEPCGN_26315at 4010.897 kb on + strand, within HEPCGN_26315at 4010.901 kb on - strand, within HEPCGN_26315at 4011.002 kb on - strandat 4011.002 kb on - strandat 4011.025 kb on + strandat 4011.030 kb on - strandat 4011.036 kb on + strandat 4011.122 kb on + strandat 4011.123 kb on - strandat 4011.123 kb on - strandat 4011.220 kb on - strandat 4011.220 kb on - strandat 4011.860 kb on - strand, within tnp-IS629at 4012.554 kb on + strand, within HEPCGN_26330at 4012.683 kb on - strand, within HEPCGN_26330at 4012.815 kb on + strandat 4012.838 kb on + strandat 4012.862 kb on + strandat 4012.932 kb on + strandat 4013.002 kb on + strandat 4013.002 kb on + strandat 4013.048 kb on + strandat 4013.051 kb on + strandat 4013.110 kb on + strandat 4013.110 kb on + strandat 4013.111 kb on - strandat 4013.111 kb on - strandat 4013.182 kb on - strandat 4013.326 kb on - strand, within pptAat 4013.381 kb on - strandat 4013.390 kb on - strandat 4013.397 kb on - strandat 4013.457 kb on + strand, within yddH

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas45
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4,010,758 + HEPCGN_26315 0.75 -0.5
4,010,861 + HEPCGN_26315 0.82 -1.6
4,010,861 + HEPCGN_26315 0.82 +0.5
4,010,862 - HEPCGN_26315 0.82 -2.0
4,010,862 - HEPCGN_26315 0.82 -0.1
4,010,862 - HEPCGN_26315 0.82 +0.4
4,010,897 + HEPCGN_26315 0.84 -0.6
4,010,901 - HEPCGN_26315 0.84 -0.3
4,011,002 - +0.2
4,011,002 - -0.2
4,011,025 + -1.9
4,011,030 - +1.0
4,011,036 + -2.8
4,011,122 + +0.9
4,011,123 - +0.6
4,011,123 - +0.2
4,011,220 - -0.7
4,011,220 - -1.3
4,011,860 - tnp-IS629 HEPCGN_26325 0.28 +1.3
4,012,554 + HEPCGN_26330 0.37 -0.4
4,012,683 - HEPCGN_26330 0.63 -0.6
4,012,815 + -0.7
4,012,838 + +2.4
4,012,862 + -0.1
4,012,932 + -0.2
4,013,002 + -0.1
4,013,002 + +0.4
4,013,048 + -0.6
4,013,051 + -2.2
4,013,110 + -1.2
4,013,110 + -1.0
4,013,111 - -1.1
4,013,111 - -1.3
4,013,182 - +1.2
4,013,326 - pptA HEPCGN_26335 0.71 +0.1
4,013,381 - +0.6
4,013,390 - +0.5
4,013,397 - -2.8
4,013,457 + yddH HEPCGN_26340 0.11 -1.0

Or see this region's nucleotide sequence