Experiment: Control_ECOR38
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrD and zapC are separated by 173 nucleotides zapC and ycbX overlap by 4 nucleotides
HEPCGN_23225: pyrD - quinone-dependent dihydroorotate dehydrogenase, at 3,439,632 to 3,440,642
pyrD
HEPCGN_23230: zapC - cell division protein ZapC, at 3,440,816 to 3,441,358
zapC
HEPCGN_23235: ycbX - 6-N-hydroxylaminopurine resistance protein YcbX, at 3,441,355 to 3,442,464
ycbX
Position (kb)
3440
3441
3442 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3439.980 kb on - strand, within pyrD at 3440.045 kb on + strand, within pyrD at 3440.125 kb on - strand, within pyrD at 3440.147 kb on + strand, within pyrD at 3440.259 kb on - strand, within pyrD at 3440.262 kb on + strand, within pyrD at 3440.346 kb on - strand, within pyrD at 3440.428 kb on + strand, within pyrD at 3440.664 kb on + strand at 3440.671 kb on + strand at 3440.705 kb on - strand at 3440.706 kb on + strand at 3440.797 kb on + strand at 3441.021 kb on + strand, within zapC at 3441.025 kb on + strand, within zapC at 3441.052 kb on + strand, within zapC at 3441.226 kb on - strand, within zapC at 3441.369 kb on + strand at 3441.369 kb on + strand at 3441.370 kb on - strand at 3441.370 kb on - strand at 3441.394 kb on - strand at 3441.556 kb on - strand, within ycbX at 3441.589 kb on - strand, within ycbX at 3441.592 kb on + strand, within ycbX at 3441.593 kb on - strand, within ycbX at 3441.657 kb on + strand, within ycbX at 3441.657 kb on + strand, within ycbX at 3441.906 kb on + strand, within ycbX at 3442.000 kb on + strand, within ycbX at 3442.001 kb on - strand, within ycbX at 3442.054 kb on + strand, within ycbX at 3442.054 kb on + strand, within ycbX at 3442.235 kb on + strand, within ycbX at 3442.235 kb on + strand, within ycbX at 3442.235 kb on + strand, within ycbX at 3442.235 kb on + strand, within ycbX at 3442.236 kb on - strand, within ycbX at 3442.236 kb on - strand, within ycbX at 3442.236 kb on - strand, within ycbX at 3442.293 kb on + strand, within ycbX at 3442.329 kb on + strand, within ycbX at 3442.330 kb on - strand, within ycbX at 3442.336 kb on - strand, within ycbX at 3442.356 kb on + strand at 3442.357 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECOR38 remove 3,439,980 - pyrD HEPCGN_23225 0.34 +0.2 3,440,045 + pyrD HEPCGN_23225 0.41 +0.4 3,440,125 - pyrD HEPCGN_23225 0.49 +1.6 3,440,147 + pyrD HEPCGN_23225 0.51 -0.6 3,440,259 - pyrD HEPCGN_23225 0.62 -0.7 3,440,262 + pyrD HEPCGN_23225 0.62 +0.1 3,440,346 - pyrD HEPCGN_23225 0.71 -1.4 3,440,428 + pyrD HEPCGN_23225 0.79 -0.5 3,440,664 + -0.5 3,440,671 + -0.2 3,440,705 - -2.1 3,440,706 + -0.4 3,440,797 + +1.4 3,441,021 + zapC HEPCGN_23230 0.38 -0.2 3,441,025 + zapC HEPCGN_23230 0.38 -0.6 3,441,052 + zapC HEPCGN_23230 0.43 -1.6 3,441,226 - zapC HEPCGN_23230 0.76 +0.2 3,441,369 + -0.3 3,441,369 + -0.5 3,441,370 - +1.2 3,441,370 - -0.9 3,441,394 - -0.1 3,441,556 - ycbX HEPCGN_23235 0.18 -0.1 3,441,589 - ycbX HEPCGN_23235 0.21 +0.8 3,441,592 + ycbX HEPCGN_23235 0.21 -1.1 3,441,593 - ycbX HEPCGN_23235 0.21 +0.2 3,441,657 + ycbX HEPCGN_23235 0.27 +0.3 3,441,657 + ycbX HEPCGN_23235 0.27 +0.2 3,441,906 + ycbX HEPCGN_23235 0.50 +1.3 3,442,000 + ycbX HEPCGN_23235 0.58 +1.0 3,442,001 - ycbX HEPCGN_23235 0.58 +0.3 3,442,054 + ycbX HEPCGN_23235 0.63 -2.2 3,442,054 + ycbX HEPCGN_23235 0.63 -0.7 3,442,235 + ycbX HEPCGN_23235 0.79 +0.2 3,442,235 + ycbX HEPCGN_23235 0.79 +0.7 3,442,235 + ycbX HEPCGN_23235 0.79 -0.8 3,442,235 + ycbX HEPCGN_23235 0.79 +0.3 3,442,236 - ycbX HEPCGN_23235 0.79 +2.7 3,442,236 - ycbX HEPCGN_23235 0.79 -1.2 3,442,236 - ycbX HEPCGN_23235 0.79 +1.1 3,442,293 + ycbX HEPCGN_23235 0.85 -0.2 3,442,329 + ycbX HEPCGN_23235 0.88 -0.0 3,442,330 - ycbX HEPCGN_23235 0.88 -0.8 3,442,336 - ycbX HEPCGN_23235 0.88 +1.2 3,442,356 + -0.1 3,442,357 - -1.0
Or see this region's nucleotide sequence