Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lsrF and lsrG are separated by 23 nucleotides lsrG and tam are separated by 56 nucleotides tam and yneE are separated by 3 nucleotides
HEPCGN_26610: lsrF - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 4,078,887 to 4,079,762
lsrF
HEPCGN_26615: lsrG - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 4,079,786 to 4,080,076
lsrG
HEPCGN_26620: tam - trans-aconitate 2-methyltransferase, at 4,080,133 to 4,080,891
tam
HEPCGN_26625: yneE - UPF0187 protein YneE, at 4,080,895 to 4,081,809
yneE
Position (kb) 4080
4081 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4079.138 kb on - strand, within lsrF at 4079.141 kb on - strand, within lsrF at 4079.141 kb on - strand, within lsrF at 4079.147 kb on + strand, within lsrF at 4079.170 kb on + strand, within lsrF at 4079.490 kb on - strand, within lsrF at 4079.500 kb on - strand, within lsrF at 4079.939 kb on - strand, within lsrG at 4079.980 kb on + strand, within lsrG at 4079.981 kb on - strand, within lsrG at 4079.981 kb on - strand, within lsrG at 4080.103 kb on + strand at 4080.133 kb on - strand at 4080.232 kb on + strand, within tam at 4080.625 kb on + strand, within tam at 4080.629 kb on - strand, within tam at 4080.679 kb on + strand, within tam at 4080.680 kb on - strand, within tam at 4080.737 kb on + strand, within tam at 4081.066 kb on - strand, within yneE at 4081.092 kb on + strand, within yneE at 4081.092 kb on + strand, within yneE at 4081.093 kb on - strand, within yneE at 4081.106 kb on + strand, within yneE at 4081.144 kb on - strand, within yneE at 4081.182 kb on - strand, within yneE at 4081.328 kb on - strand, within yneE at 4081.391 kb on + strand, within yneE at 4081.392 kb on - strand, within yneE at 4081.480 kb on + strand, within yneE at 4081.594 kb on - strand, within yneE at 4081.696 kb on - strand, within yneE at 4081.814 kb on - strand at 4081.853 kb on - strand at 4081.875 kb on - strand at 4081.885 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 4,079,138 - lsrF HEPCGN_26610 0.29 +2.3 4,079,141 - lsrF HEPCGN_26610 0.29 -1.9 4,079,141 - lsrF HEPCGN_26610 0.29 -1.6 4,079,147 + lsrF HEPCGN_26610 0.30 +1.5 4,079,170 + lsrF HEPCGN_26610 0.32 +2.6 4,079,490 - lsrF HEPCGN_26610 0.69 -2.4 4,079,500 - lsrF HEPCGN_26610 0.70 +0.6 4,079,939 - lsrG HEPCGN_26615 0.53 +2.5 4,079,980 + lsrG HEPCGN_26615 0.67 -0.6 4,079,981 - lsrG HEPCGN_26615 0.67 -0.3 4,079,981 - lsrG HEPCGN_26615 0.67 +0.2 4,080,103 + +0.3 4,080,133 - +0.7 4,080,232 + tam HEPCGN_26620 0.13 +0.6 4,080,625 + tam HEPCGN_26620 0.65 +0.5 4,080,629 - tam HEPCGN_26620 0.65 +0.7 4,080,679 + tam HEPCGN_26620 0.72 -1.3 4,080,680 - tam HEPCGN_26620 0.72 +0.1 4,080,737 + tam HEPCGN_26620 0.80 +3.0 4,081,066 - yneE HEPCGN_26625 0.19 -0.4 4,081,092 + yneE HEPCGN_26625 0.22 -0.6 4,081,092 + yneE HEPCGN_26625 0.22 -1.1 4,081,093 - yneE HEPCGN_26625 0.22 +0.9 4,081,106 + yneE HEPCGN_26625 0.23 +0.7 4,081,144 - yneE HEPCGN_26625 0.27 -0.3 4,081,182 - yneE HEPCGN_26625 0.31 -1.3 4,081,328 - yneE HEPCGN_26625 0.47 +1.6 4,081,391 + yneE HEPCGN_26625 0.54 -0.8 4,081,392 - yneE HEPCGN_26625 0.54 +0.3 4,081,480 + yneE HEPCGN_26625 0.64 +0.0 4,081,594 - yneE HEPCGN_26625 0.76 -2.0 4,081,696 - yneE HEPCGN_26625 0.88 -1.7 4,081,814 - +0.9 4,081,853 - +4.3 4,081,875 - +0.3 4,081,885 + +1.6
Or see this region's nucleotide sequence