Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lsrB and lsrF are separated by 26 nucleotides lsrF and lsrG are separated by 23 nucleotides lsrG and tam are separated by 56 nucleotides
HEPCGN_26605: lsrB - autoinducer 2 ABC transporter substrate-binding protein LsrB, at 4,077,838 to 4,078,860
lsrB
HEPCGN_26610: lsrF - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 4,078,887 to 4,079,762
lsrF
HEPCGN_26615: lsrG - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 4,079,786 to 4,080,076
lsrG
HEPCGN_26620: tam - trans-aconitate 2-methyltransferase, at 4,080,133 to 4,080,891
tam
Position (kb) 4078
4079
4080 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4077.893 kb on + strand at 4078.059 kb on - strand, within lsrB at 4078.216 kb on + strand, within lsrB at 4078.221 kb on + strand, within lsrB at 4078.222 kb on - strand, within lsrB at 4078.252 kb on - strand, within lsrB at 4078.252 kb on - strand, within lsrB at 4078.460 kb on + strand, within lsrB at 4078.471 kb on - strand, within lsrB at 4078.667 kb on - strand, within lsrB at 4078.802 kb on + strand at 4078.802 kb on + strand at 4078.823 kb on + strand at 4078.849 kb on + strand at 4078.868 kb on + strand at 4078.869 kb on - strand at 4078.882 kb on - strand at 4079.138 kb on - strand, within lsrF at 4079.141 kb on - strand, within lsrF at 4079.141 kb on - strand, within lsrF at 4079.147 kb on + strand, within lsrF at 4079.170 kb on + strand, within lsrF at 4079.490 kb on - strand, within lsrF at 4079.500 kb on - strand, within lsrF at 4079.939 kb on - strand, within lsrG at 4079.980 kb on + strand, within lsrG at 4079.981 kb on - strand, within lsrG at 4079.981 kb on - strand, within lsrG at 4080.103 kb on + strand at 4080.133 kb on - strand at 4080.232 kb on + strand, within tam at 4080.625 kb on + strand, within tam at 4080.629 kb on - strand, within tam at 4080.679 kb on + strand, within tam at 4080.680 kb on - strand, within tam at 4080.737 kb on + strand, within tam
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 4,077,893 + -0.5 4,078,059 - lsrB HEPCGN_26605 0.22 +1.3 4,078,216 + lsrB HEPCGN_26605 0.37 +2.5 4,078,221 + lsrB HEPCGN_26605 0.37 +0.2 4,078,222 - lsrB HEPCGN_26605 0.38 -0.5 4,078,252 - lsrB HEPCGN_26605 0.40 -0.2 4,078,252 - lsrB HEPCGN_26605 0.40 +0.3 4,078,460 + lsrB HEPCGN_26605 0.61 +0.9 4,078,471 - lsrB HEPCGN_26605 0.62 +0.9 4,078,667 - lsrB HEPCGN_26605 0.81 +0.9 4,078,802 + -0.7 4,078,802 + -0.2 4,078,823 + -0.1 4,078,849 + +0.2 4,078,868 + -0.2 4,078,869 - -0.1 4,078,882 - -1.7 4,079,138 - lsrF HEPCGN_26610 0.29 +2.3 4,079,141 - lsrF HEPCGN_26610 0.29 -1.9 4,079,141 - lsrF HEPCGN_26610 0.29 -1.6 4,079,147 + lsrF HEPCGN_26610 0.30 +1.5 4,079,170 + lsrF HEPCGN_26610 0.32 +2.6 4,079,490 - lsrF HEPCGN_26610 0.69 -2.4 4,079,500 - lsrF HEPCGN_26610 0.70 +0.6 4,079,939 - lsrG HEPCGN_26615 0.53 +2.5 4,079,980 + lsrG HEPCGN_26615 0.67 -0.6 4,079,981 - lsrG HEPCGN_26615 0.67 -0.3 4,079,981 - lsrG HEPCGN_26615 0.67 +0.2 4,080,103 + +0.3 4,080,133 - +0.7 4,080,232 + tam HEPCGN_26620 0.13 +0.6 4,080,625 + tam HEPCGN_26620 0.65 +0.5 4,080,629 - tam HEPCGN_26620 0.65 +0.7 4,080,679 + tam HEPCGN_26620 0.72 -1.3 4,080,680 - tam HEPCGN_26620 0.72 +0.1 4,080,737 + tam HEPCGN_26620 0.80 +3.0
Or see this region's nucleotide sequence