Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pptA and yddH are separated by 3 nucleotides yddH and nhoA are separated by 174 nucleotides nhoA and yddE are separated by 95 nucleotides
HEPCGN_26335: pptA - tautomerase PptA, at 4,013,164 to 4,013,391
pptA
HEPCGN_26340: yddH - Uncharacterized protein YddH, at 4,013,395 to 4,013,964
yddH
HEPCGN_26345: nhoA - N-hydroxyarylamine O-acetyltransferase, at 4,014,139 to 4,014,984
nhoA
HEPCGN_26350: yddE - PhzF family isomerase, at 4,015,080 to 4,015,973
yddE
Position (kb) 4014
4015 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4013.182 kb on - strand at 4013.326 kb on - strand, within pptA at 4013.381 kb on - strand at 4013.390 kb on - strand at 4013.397 kb on - strand at 4013.457 kb on + strand, within yddH at 4013.760 kb on + strand, within yddH at 4013.794 kb on - strand, within yddH at 4013.794 kb on - strand, within yddH at 4013.798 kb on - strand, within yddH at 4013.862 kb on + strand, within yddH at 4013.939 kb on - strand at 4014.080 kb on - strand at 4014.105 kb on + strand at 4014.106 kb on - strand at 4014.107 kb on + strand at 4014.108 kb on - strand at 4014.108 kb on - strand at 4014.174 kb on + strand at 4014.285 kb on - strand, within nhoA at 4014.323 kb on + strand, within nhoA at 4014.323 kb on + strand, within nhoA at 4014.393 kb on + strand, within nhoA at 4014.425 kb on + strand, within nhoA at 4014.443 kb on + strand, within nhoA at 4014.805 kb on - strand, within nhoA at 4014.831 kb on + strand, within nhoA at 4014.863 kb on + strand, within nhoA at 4014.864 kb on - strand, within nhoA at 4014.865 kb on + strand, within nhoA at 4014.866 kb on - strand, within nhoA at 4015.011 kb on + strand at 4015.034 kb on + strand at 4015.251 kb on + strand, within yddE at 4015.263 kb on + strand, within yddE at 4015.266 kb on + strand, within yddE at 4015.266 kb on + strand, within yddE at 4015.319 kb on + strand, within yddE at 4015.655 kb on + strand, within yddE at 4015.728 kb on + strand, within yddE at 4015.729 kb on - strand, within yddE at 4015.732 kb on + strand, within yddE at 4015.785 kb on - strand, within yddE at 4015.941 kb on - strand at 4015.975 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 4,013,182 - +1.4 4,013,326 - pptA HEPCGN_26335 0.71 -0.4 4,013,381 - -0.0 4,013,390 - +0.2 4,013,397 - -1.3 4,013,457 + yddH HEPCGN_26340 0.11 -2.8 4,013,760 + yddH HEPCGN_26340 0.64 -0.9 4,013,794 - yddH HEPCGN_26340 0.70 +0.5 4,013,794 - yddH HEPCGN_26340 0.70 +0.0 4,013,798 - yddH HEPCGN_26340 0.71 -0.1 4,013,862 + yddH HEPCGN_26340 0.82 +0.3 4,013,939 - +2.6 4,014,080 - +0.5 4,014,105 + -0.9 4,014,106 - +1.1 4,014,107 + -0.9 4,014,108 - +0.4 4,014,108 - +0.3 4,014,174 + +0.1 4,014,285 - nhoA HEPCGN_26345 0.17 +0.8 4,014,323 + nhoA HEPCGN_26345 0.22 +0.0 4,014,323 + nhoA HEPCGN_26345 0.22 -0.8 4,014,393 + nhoA HEPCGN_26345 0.30 +0.5 4,014,425 + nhoA HEPCGN_26345 0.34 -0.2 4,014,443 + nhoA HEPCGN_26345 0.36 +3.5 4,014,805 - nhoA HEPCGN_26345 0.79 +1.0 4,014,831 + nhoA HEPCGN_26345 0.82 +1.1 4,014,863 + nhoA HEPCGN_26345 0.86 +0.7 4,014,864 - nhoA HEPCGN_26345 0.86 +1.2 4,014,865 + nhoA HEPCGN_26345 0.86 -1.9 4,014,866 - nhoA HEPCGN_26345 0.86 +0.9 4,015,011 + -0.4 4,015,034 + -0.3 4,015,251 + yddE HEPCGN_26350 0.19 +1.0 4,015,263 + yddE HEPCGN_26350 0.20 -0.1 4,015,266 + yddE HEPCGN_26350 0.21 +1.5 4,015,266 + yddE HEPCGN_26350 0.21 +2.8 4,015,319 + yddE HEPCGN_26350 0.27 +0.4 4,015,655 + yddE HEPCGN_26350 0.64 -0.1 4,015,728 + yddE HEPCGN_26350 0.72 +0.1 4,015,729 - yddE HEPCGN_26350 0.73 -0.4 4,015,732 + yddE HEPCGN_26350 0.73 -0.1 4,015,785 - yddE HEPCGN_26350 0.79 -1.3 4,015,941 - -0.9 4,015,975 - -1.9
Or see this region's nucleotide sequence