Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tyrR and tpx are separated by 43 nucleotides tpx and ycjG are separated by 118 nucleotides ycjG and mpaA overlap by 26 nucleotides mpaA and mppA are separated by 182 nucleotides
HEPCGN_25885: tyrR - transcriptional regulator TyrR, at 3,917,655 to 3,919,196
tyrR
HEPCGN_25890: tpx - thiol peroxidase, at 3,919,240 to 3,919,746
tpx
HEPCGN_25895: ycjG - L-Ala-D/L-Glu epimerase, at 3,919,865 to 3,920,830
ycjG
HEPCGN_25900: mpaA - murein tripeptide amidase MpaA, at 3,920,805 to 3,921,533
mpaA
HEPCGN_25905: mppA - murein tripeptide ABC transporter substrate-binding protein MppA, at 3,921,716 to 3,923,329
mppA
Position (kb)
3919
3920
3921 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3918.921 kb on - strand, within tyrR at 3918.924 kb on + strand, within tyrR at 3918.987 kb on + strand, within tyrR at 3918.988 kb on - strand, within tyrR at 3919.046 kb on + strand at 3919.072 kb on + strand at 3919.236 kb on + strand at 3919.237 kb on - strand at 3919.242 kb on - strand at 3919.395 kb on - strand, within tpx at 3919.438 kb on - strand, within tpx at 3919.549 kb on - strand, within tpx at 3919.763 kb on + strand at 3919.763 kb on + strand at 3919.763 kb on + strand at 3919.768 kb on - strand at 3919.768 kb on - strand at 3919.769 kb on + strand at 3919.785 kb on + strand at 3919.791 kb on - strand at 3919.821 kb on - strand at 3919.861 kb on - strand at 3919.881 kb on + strand at 3919.881 kb on + strand at 3919.882 kb on - strand at 3919.903 kb on - strand at 3919.906 kb on + strand at 3920.015 kb on + strand, within ycjG at 3920.333 kb on - strand, within ycjG at 3920.439 kb on + strand, within ycjG at 3920.551 kb on + strand, within ycjG at 3920.552 kb on - strand, within ycjG at 3920.558 kb on - strand, within ycjG at 3920.698 kb on + strand, within ycjG at 3920.829 kb on - strand at 3920.969 kb on - strand, within mpaA at 3921.022 kb on - strand, within mpaA at 3921.022 kb on - strand, within mpaA at 3921.047 kb on + strand, within mpaA at 3921.100 kb on - strand, within mpaA at 3921.101 kb on + strand, within mpaA at 3921.101 kb on + strand, within mpaA at 3921.101 kb on + strand, within mpaA at 3921.102 kb on - strand, within mpaA at 3921.102 kb on - strand, within mpaA at 3921.448 kb on - strand, within mpaA at 3921.555 kb on + strand at 3921.556 kb on - strand at 3921.556 kb on - strand at 3921.556 kb on - strand at 3921.556 kb on - strand at 3921.589 kb on - strand at 3921.620 kb on + strand at 3921.715 kb on + strand at 3921.776 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 3,918,921 - tyrR HEPCGN_25885 0.82 -1.2 3,918,924 + tyrR HEPCGN_25885 0.82 -1.7 3,918,987 + tyrR HEPCGN_25885 0.86 -0.4 3,918,988 - tyrR HEPCGN_25885 0.86 +0.0 3,919,046 + -1.3 3,919,072 + -0.2 3,919,236 + +0.1 3,919,237 - -2.1 3,919,242 - -0.3 3,919,395 - tpx HEPCGN_25890 0.31 -0.3 3,919,438 - tpx HEPCGN_25890 0.39 +0.7 3,919,549 - tpx HEPCGN_25890 0.61 +0.1 3,919,763 + +0.4 3,919,763 + -0.4 3,919,763 + +3.6 3,919,768 - +0.2 3,919,768 - +0.3 3,919,769 + +1.4 3,919,785 + -0.4 3,919,791 - -0.9 3,919,821 - -0.0 3,919,861 - +0.2 3,919,881 + +0.8 3,919,881 + -1.5 3,919,882 - -0.1 3,919,903 - +1.0 3,919,906 + -0.4 3,920,015 + ycjG HEPCGN_25895 0.16 +0.2 3,920,333 - ycjG HEPCGN_25895 0.48 +0.2 3,920,439 + ycjG HEPCGN_25895 0.59 +1.6 3,920,551 + ycjG HEPCGN_25895 0.71 +1.7 3,920,552 - ycjG HEPCGN_25895 0.71 +1.9 3,920,558 - ycjG HEPCGN_25895 0.72 +1.7 3,920,698 + ycjG HEPCGN_25895 0.86 -1.4 3,920,829 - +0.9 3,920,969 - mpaA HEPCGN_25900 0.22 -0.6 3,921,022 - mpaA HEPCGN_25900 0.30 +0.1 3,921,022 - mpaA HEPCGN_25900 0.30 -0.8 3,921,047 + mpaA HEPCGN_25900 0.33 +0.5 3,921,100 - mpaA HEPCGN_25900 0.40 +1.0 3,921,101 + mpaA HEPCGN_25900 0.41 +0.0 3,921,101 + mpaA HEPCGN_25900 0.41 +1.4 3,921,101 + mpaA HEPCGN_25900 0.41 -0.4 3,921,102 - mpaA HEPCGN_25900 0.41 -0.1 3,921,102 - mpaA HEPCGN_25900 0.41 +0.8 3,921,448 - mpaA HEPCGN_25900 0.88 -0.8 3,921,555 + -1.9 3,921,556 - -1.2 3,921,556 - -1.1 3,921,556 - -1.8 3,921,556 - +0.5 3,921,589 - -0.7 3,921,620 + -0.3 3,921,715 + +0.9 3,921,776 + -1.0
Or see this region's nucleotide sequence