Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sohB and yciN are separated by 35 nucleotides yciN and HEPCGN_25595 are separated by 379 nucleotides HEPCGN_25595 and cysB are separated by 209 nucleotides
HEPCGN_25585: sohB - protease SohB, at 3,853,347 to 3,854,396
sohB
HEPCGN_25590: yciN - Protein YciN, at 3,854,432 to 3,854,683
yciN
HEPCGN_25595: HEPCGN_25595 - type I DNA topoisomerase, at 3,855,063 to 3,857,660
_25595
HEPCGN_25600: cysB - HTH-type transcriptional regulator CysB, at 3,857,870 to 3,858,844
cysB
Position (kb)
3855
3856
3857
3858 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3854.138 kb on + strand, within sohB at 3854.139 kb on - strand, within sohB at 3854.246 kb on + strand, within sohB at 3854.289 kb on + strand, within sohB at 3854.394 kb on + strand at 3854.413 kb on + strand at 3854.434 kb on - strand at 3854.434 kb on - strand at 3854.485 kb on + strand, within yciN at 3854.485 kb on + strand, within yciN at 3854.486 kb on - strand, within yciN at 3854.486 kb on - strand, within yciN at 3854.489 kb on - strand, within yciN at 3854.709 kb on - strand at 3854.740 kb on - strand at 3854.740 kb on - strand at 3854.741 kb on + strand at 3854.741 kb on + strand at 3854.766 kb on + strand at 3854.815 kb on + strand at 3854.991 kb on + strand at 3855.014 kb on + strand at 3855.015 kb on - strand at 3855.049 kb on + strand at 3857.244 kb on + strand, within HEPCGN_25595 at 3857.245 kb on - strand, within HEPCGN_25595 at 3857.245 kb on - strand, within HEPCGN_25595 at 3857.245 kb on - strand, within HEPCGN_25595 at 3857.245 kb on - strand, within HEPCGN_25595 at 3857.245 kb on - strand, within HEPCGN_25595 at 3857.306 kb on - strand, within HEPCGN_25595 at 3857.335 kb on + strand, within HEPCGN_25595 at 3857.367 kb on + strand, within HEPCGN_25595 at 3857.367 kb on + strand, within HEPCGN_25595 at 3857.367 kb on + strand, within HEPCGN_25595 at 3857.367 kb on + strand, within HEPCGN_25595 at 3857.534 kb on + strand at 3857.534 kb on + strand at 3857.537 kb on + strand at 3857.625 kb on + strand at 3857.742 kb on + strand at 3857.743 kb on - strand at 3857.743 kb on - strand at 3857.744 kb on + strand at 3857.757 kb on + strand at 3857.758 kb on - strand at 3857.758 kb on - strand at 3857.764 kb on + strand at 3857.767 kb on - strand at 3858.185 kb on + strand, within cysB at 3858.459 kb on - strand, within cysB at 3858.460 kb on + strand, within cysB at 3858.460 kb on + strand, within cysB at 3858.461 kb on - strand, within cysB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 3,854,138 + sohB HEPCGN_25585 0.75 -0.1 3,854,139 - sohB HEPCGN_25585 0.75 -2.4 3,854,246 + sohB HEPCGN_25585 0.86 +2.2 3,854,289 + sohB HEPCGN_25585 0.90 +0.1 3,854,394 + -0.6 3,854,413 + -0.0 3,854,434 - +2.4 3,854,434 - -2.2 3,854,485 + yciN HEPCGN_25590 0.21 +0.7 3,854,485 + yciN HEPCGN_25590 0.21 -0.8 3,854,486 - yciN HEPCGN_25590 0.21 +0.8 3,854,486 - yciN HEPCGN_25590 0.21 -1.1 3,854,489 - yciN HEPCGN_25590 0.23 +0.4 3,854,709 - -1.5 3,854,740 - -0.6 3,854,740 - -0.4 3,854,741 + +2.6 3,854,741 + -1.6 3,854,766 + -0.6 3,854,815 + +0.0 3,854,991 + +0.8 3,855,014 + -2.2 3,855,015 - -2.5 3,855,049 + -1.6 3,857,244 + HEPCGN_25595 0.84 -1.1 3,857,245 - HEPCGN_25595 0.84 -0.3 3,857,245 - HEPCGN_25595 0.84 +0.5 3,857,245 - HEPCGN_25595 0.84 +0.5 3,857,245 - HEPCGN_25595 0.84 -1.3 3,857,245 - HEPCGN_25595 0.84 +0.9 3,857,306 - HEPCGN_25595 0.86 -1.8 3,857,335 + HEPCGN_25595 0.87 -1.3 3,857,367 + HEPCGN_25595 0.89 +0.5 3,857,367 + HEPCGN_25595 0.89 +0.6 3,857,367 + HEPCGN_25595 0.89 +2.5 3,857,367 + HEPCGN_25595 0.89 -0.1 3,857,534 + -0.4 3,857,534 + -0.9 3,857,537 + +0.4 3,857,625 + -1.3 3,857,742 + -0.4 3,857,743 - +0.1 3,857,743 - -0.1 3,857,744 + +0.2 3,857,757 + -0.1 3,857,758 - -0.1 3,857,758 - -0.8 3,857,764 + +0.4 3,857,767 - -0.3 3,858,185 + cysB HEPCGN_25600 0.32 +1.1 3,858,459 - cysB HEPCGN_25600 0.60 -0.8 3,858,460 + cysB HEPCGN_25600 0.61 +2.0 3,858,460 + cysB HEPCGN_25600 0.61 +0.7 3,858,461 - cysB HEPCGN_25600 0.61 +1.1
Or see this region's nucleotide sequence