Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpB and trpCF are separated by 11 nucleotides trpCF and trpD are separated by 3 nucleotides
HEPCGN_25520: trpB - tryptophan synthase subunit beta, at 3,839,741 to 3,840,934
trpB
HEPCGN_25525: trpCF - bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF, at 3,840,946 to 3,842,304
trpCF
HEPCGN_25530: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 3,842,308 to 3,843,903
trpD
Position (kb)
3840
3841
3842
3843 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3839.957 kb on - strand, within trpB at 3839.964 kb on + strand, within trpB at 3839.965 kb on - strand, within trpB at 3839.992 kb on - strand, within trpB at 3840.042 kb on + strand, within trpB at 3840.042 kb on + strand, within trpB at 3840.043 kb on - strand, within trpB at 3840.043 kb on - strand, within trpB at 3840.100 kb on - strand, within trpB at 3840.376 kb on + strand, within trpB at 3840.376 kb on + strand, within trpB at 3840.376 kb on + strand, within trpB at 3840.377 kb on - strand, within trpB at 3840.378 kb on + strand, within trpB at 3840.378 kb on + strand, within trpB at 3840.379 kb on - strand, within trpB at 3840.536 kb on - strand, within trpB at 3840.537 kb on + strand, within trpB at 3840.537 kb on + strand, within trpB at 3840.670 kb on + strand, within trpB at 3840.670 kb on + strand, within trpB at 3840.671 kb on - strand, within trpB at 3840.780 kb on + strand, within trpB at 3840.888 kb on + strand at 3840.888 kb on + strand at 3840.889 kb on - strand at 3840.920 kb on - strand at 3840.923 kb on + strand at 3840.924 kb on - strand at 3840.924 kb on - strand at 3840.948 kb on - strand at 3840.950 kb on + strand at 3840.950 kb on + strand at 3841.027 kb on - strand at 3841.082 kb on - strand, within trpCF at 3841.179 kb on - strand, within trpCF at 3841.228 kb on + strand, within trpCF at 3841.229 kb on - strand, within trpCF at 3841.283 kb on + strand, within trpCF at 3841.284 kb on - strand, within trpCF at 3841.300 kb on - strand, within trpCF at 3841.335 kb on + strand, within trpCF at 3841.335 kb on + strand, within trpCF at 3841.501 kb on - strand, within trpCF at 3841.533 kb on + strand, within trpCF at 3841.647 kb on - strand, within trpCF at 3841.647 kb on - strand, within trpCF at 3841.651 kb on - strand, within trpCF at 3841.673 kb on - strand, within trpCF at 3841.900 kb on + strand, within trpCF at 3841.900 kb on + strand, within trpCF at 3841.900 kb on + strand, within trpCF at 3841.900 kb on + strand, within trpCF at 3841.901 kb on - strand, within trpCF at 3841.923 kb on - strand, within trpCF at 3841.969 kb on + strand, within trpCF at 3842.343 kb on - strand at 3842.419 kb on + strand at 3842.420 kb on - strand at 3842.541 kb on - strand, within trpD at 3842.577 kb on - strand, within trpD at 3842.727 kb on - strand, within trpD at 3842.767 kb on + strand, within trpD at 3842.768 kb on - strand, within trpD at 3842.768 kb on - strand, within trpD at 3842.846 kb on + strand, within trpD at 3842.876 kb on - strand, within trpD at 3842.923 kb on - strand, within trpD at 3843.039 kb on + strand, within trpD at 3843.039 kb on + strand, within trpD at 3843.092 kb on + strand, within trpD
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 3,839,957 - trpB HEPCGN_25520 0.18 -0.3 3,839,964 + trpB HEPCGN_25520 0.19 +0.1 3,839,965 - trpB HEPCGN_25520 0.19 -0.3 3,839,992 - trpB HEPCGN_25520 0.21 -0.4 3,840,042 + trpB HEPCGN_25520 0.25 +2.6 3,840,042 + trpB HEPCGN_25520 0.25 -0.8 3,840,043 - trpB HEPCGN_25520 0.25 -0.9 3,840,043 - trpB HEPCGN_25520 0.25 +0.8 3,840,100 - trpB HEPCGN_25520 0.30 -0.6 3,840,376 + trpB HEPCGN_25520 0.53 -1.5 3,840,376 + trpB HEPCGN_25520 0.53 +0.2 3,840,376 + trpB HEPCGN_25520 0.53 -0.7 3,840,377 - trpB HEPCGN_25520 0.53 -2.5 3,840,378 + trpB HEPCGN_25520 0.53 -0.8 3,840,378 + trpB HEPCGN_25520 0.53 -0.0 3,840,379 - trpB HEPCGN_25520 0.53 -0.0 3,840,536 - trpB HEPCGN_25520 0.67 +0.1 3,840,537 + trpB HEPCGN_25520 0.67 +0.8 3,840,537 + trpB HEPCGN_25520 0.67 +0.5 3,840,670 + trpB HEPCGN_25520 0.78 +1.2 3,840,670 + trpB HEPCGN_25520 0.78 +1.8 3,840,671 - trpB HEPCGN_25520 0.78 -0.5 3,840,780 + trpB HEPCGN_25520 0.87 -0.9 3,840,888 + -0.8 3,840,888 + -1.3 3,840,889 - -0.8 3,840,920 - -0.6 3,840,923 + -1.1 3,840,924 - +0.7 3,840,924 - +0.1 3,840,948 - +1.0 3,840,950 + +0.3 3,840,950 + -0.4 3,841,027 - +0.5 3,841,082 - trpCF HEPCGN_25525 0.10 +0.5 3,841,179 - trpCF HEPCGN_25525 0.17 +0.5 3,841,228 + trpCF HEPCGN_25525 0.21 -0.1 3,841,229 - trpCF HEPCGN_25525 0.21 +0.2 3,841,283 + trpCF HEPCGN_25525 0.25 +0.5 3,841,284 - trpCF HEPCGN_25525 0.25 -0.5 3,841,300 - trpCF HEPCGN_25525 0.26 -0.7 3,841,335 + trpCF HEPCGN_25525 0.29 +0.9 3,841,335 + trpCF HEPCGN_25525 0.29 -1.1 3,841,501 - trpCF HEPCGN_25525 0.41 +1.1 3,841,533 + trpCF HEPCGN_25525 0.43 +0.2 3,841,647 - trpCF HEPCGN_25525 0.52 +1.7 3,841,647 - trpCF HEPCGN_25525 0.52 -0.8 3,841,651 - trpCF HEPCGN_25525 0.52 +0.8 3,841,673 - trpCF HEPCGN_25525 0.53 +3.2 3,841,900 + trpCF HEPCGN_25525 0.70 +0.4 3,841,900 + trpCF HEPCGN_25525 0.70 -0.6 3,841,900 + trpCF HEPCGN_25525 0.70 -1.1 3,841,900 + trpCF HEPCGN_25525 0.70 +4.3 3,841,901 - trpCF HEPCGN_25525 0.70 +0.7 3,841,923 - trpCF HEPCGN_25525 0.72 -0.8 3,841,969 + trpCF HEPCGN_25525 0.75 -0.3 3,842,343 - -0.4 3,842,419 + -1.2 3,842,420 - +1.1 3,842,541 - trpD HEPCGN_25530 0.15 +0.8 3,842,577 - trpD HEPCGN_25530 0.17 +0.4 3,842,727 - trpD HEPCGN_25530 0.26 +0.6 3,842,767 + trpD HEPCGN_25530 0.29 -0.4 3,842,768 - trpD HEPCGN_25530 0.29 -0.6 3,842,768 - trpD HEPCGN_25530 0.29 -1.0 3,842,846 + trpD HEPCGN_25530 0.34 +1.2 3,842,876 - trpD HEPCGN_25530 0.36 -0.3 3,842,923 - trpD HEPCGN_25530 0.39 +1.0 3,843,039 + trpD HEPCGN_25530 0.46 -0.3 3,843,039 + trpD HEPCGN_25530 0.46 +0.4 3,843,092 + trpD HEPCGN_25530 0.49 +0.5
Or see this region's nucleotide sequence