Strain Fitness in Escherichia coli ECOR38 around HEPCGN_25525

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpB and trpCF are separated by 11 nucleotidestrpCF and trpD are separated by 3 nucleotides HEPCGN_25520: trpB - tryptophan synthase subunit beta, at 3,839,741 to 3,840,934 trpB HEPCGN_25525: trpCF - bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF, at 3,840,946 to 3,842,304 trpCF HEPCGN_25530: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 3,842,308 to 3,843,903 trpD Position (kb) 3840 3841 3842 3843Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3839.957 kb on - strand, within trpBat 3839.964 kb on + strand, within trpBat 3839.965 kb on - strand, within trpBat 3839.992 kb on - strand, within trpBat 3840.042 kb on + strand, within trpBat 3840.042 kb on + strand, within trpBat 3840.043 kb on - strand, within trpBat 3840.043 kb on - strand, within trpBat 3840.100 kb on - strand, within trpBat 3840.376 kb on + strand, within trpBat 3840.376 kb on + strand, within trpBat 3840.376 kb on + strand, within trpBat 3840.377 kb on - strand, within trpBat 3840.378 kb on + strand, within trpBat 3840.378 kb on + strand, within trpBat 3840.379 kb on - strand, within trpBat 3840.536 kb on - strand, within trpBat 3840.537 kb on + strand, within trpBat 3840.537 kb on + strand, within trpBat 3840.670 kb on + strand, within trpBat 3840.670 kb on + strand, within trpBat 3840.671 kb on - strand, within trpBat 3840.780 kb on + strand, within trpBat 3840.888 kb on + strandat 3840.888 kb on + strandat 3840.889 kb on - strandat 3840.920 kb on - strandat 3840.923 kb on + strandat 3840.924 kb on - strandat 3840.924 kb on - strandat 3840.948 kb on - strandat 3840.950 kb on + strandat 3840.950 kb on + strandat 3841.027 kb on - strandat 3841.082 kb on - strand, within trpCFat 3841.179 kb on - strand, within trpCFat 3841.228 kb on + strand, within trpCFat 3841.229 kb on - strand, within trpCFat 3841.283 kb on + strand, within trpCFat 3841.284 kb on - strand, within trpCFat 3841.300 kb on - strand, within trpCFat 3841.335 kb on + strand, within trpCFat 3841.335 kb on + strand, within trpCFat 3841.501 kb on - strand, within trpCFat 3841.533 kb on + strand, within trpCFat 3841.647 kb on - strand, within trpCFat 3841.647 kb on - strand, within trpCFat 3841.651 kb on - strand, within trpCFat 3841.673 kb on - strand, within trpCFat 3841.900 kb on + strand, within trpCFat 3841.900 kb on + strand, within trpCFat 3841.900 kb on + strand, within trpCFat 3841.900 kb on + strand, within trpCFat 3841.901 kb on - strand, within trpCFat 3841.923 kb on - strand, within trpCFat 3841.969 kb on + strand, within trpCFat 3842.343 kb on - strandat 3842.419 kb on + strandat 3842.420 kb on - strandat 3842.541 kb on - strand, within trpDat 3842.577 kb on - strand, within trpDat 3842.727 kb on - strand, within trpDat 3842.767 kb on + strand, within trpDat 3842.768 kb on - strand, within trpDat 3842.768 kb on - strand, within trpDat 3842.846 kb on + strand, within trpDat 3842.876 kb on - strand, within trpDat 3842.923 kb on - strand, within trpDat 3843.039 kb on + strand, within trpDat 3843.039 kb on + strand, within trpDat 3843.092 kb on + strand, within trpD

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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3,839,957 - trpB HEPCGN_25520 0.18 -0.3
3,839,964 + trpB HEPCGN_25520 0.19 +0.1
3,839,965 - trpB HEPCGN_25520 0.19 -0.3
3,839,992 - trpB HEPCGN_25520 0.21 -0.4
3,840,042 + trpB HEPCGN_25520 0.25 +2.6
3,840,042 + trpB HEPCGN_25520 0.25 -0.8
3,840,043 - trpB HEPCGN_25520 0.25 -0.9
3,840,043 - trpB HEPCGN_25520 0.25 +0.8
3,840,100 - trpB HEPCGN_25520 0.30 -0.6
3,840,376 + trpB HEPCGN_25520 0.53 -1.5
3,840,376 + trpB HEPCGN_25520 0.53 +0.2
3,840,376 + trpB HEPCGN_25520 0.53 -0.7
3,840,377 - trpB HEPCGN_25520 0.53 -2.5
3,840,378 + trpB HEPCGN_25520 0.53 -0.8
3,840,378 + trpB HEPCGN_25520 0.53 -0.0
3,840,379 - trpB HEPCGN_25520 0.53 -0.0
3,840,536 - trpB HEPCGN_25520 0.67 +0.1
3,840,537 + trpB HEPCGN_25520 0.67 +0.8
3,840,537 + trpB HEPCGN_25520 0.67 +0.5
3,840,670 + trpB HEPCGN_25520 0.78 +1.2
3,840,670 + trpB HEPCGN_25520 0.78 +1.8
3,840,671 - trpB HEPCGN_25520 0.78 -0.5
3,840,780 + trpB HEPCGN_25520 0.87 -0.9
3,840,888 + -0.8
3,840,888 + -1.3
3,840,889 - -0.8
3,840,920 - -0.6
3,840,923 + -1.1
3,840,924 - +0.7
3,840,924 - +0.1
3,840,948 - +1.0
3,840,950 + +0.3
3,840,950 + -0.4
3,841,027 - +0.5
3,841,082 - trpCF HEPCGN_25525 0.10 +0.5
3,841,179 - trpCF HEPCGN_25525 0.17 +0.5
3,841,228 + trpCF HEPCGN_25525 0.21 -0.1
3,841,229 - trpCF HEPCGN_25525 0.21 +0.2
3,841,283 + trpCF HEPCGN_25525 0.25 +0.5
3,841,284 - trpCF HEPCGN_25525 0.25 -0.5
3,841,300 - trpCF HEPCGN_25525 0.26 -0.7
3,841,335 + trpCF HEPCGN_25525 0.29 +0.9
3,841,335 + trpCF HEPCGN_25525 0.29 -1.1
3,841,501 - trpCF HEPCGN_25525 0.41 +1.1
3,841,533 + trpCF HEPCGN_25525 0.43 +0.2
3,841,647 - trpCF HEPCGN_25525 0.52 +1.7
3,841,647 - trpCF HEPCGN_25525 0.52 -0.8
3,841,651 - trpCF HEPCGN_25525 0.52 +0.8
3,841,673 - trpCF HEPCGN_25525 0.53 +3.2
3,841,900 + trpCF HEPCGN_25525 0.70 +0.4
3,841,900 + trpCF HEPCGN_25525 0.70 -0.6
3,841,900 + trpCF HEPCGN_25525 0.70 -1.1
3,841,900 + trpCF HEPCGN_25525 0.70 +4.3
3,841,901 - trpCF HEPCGN_25525 0.70 +0.7
3,841,923 - trpCF HEPCGN_25525 0.72 -0.8
3,841,969 + trpCF HEPCGN_25525 0.75 -0.3
3,842,343 - -0.4
3,842,419 + -1.2
3,842,420 - +1.1
3,842,541 - trpD HEPCGN_25530 0.15 +0.8
3,842,577 - trpD HEPCGN_25530 0.17 +0.4
3,842,727 - trpD HEPCGN_25530 0.26 +0.6
3,842,767 + trpD HEPCGN_25530 0.29 -0.4
3,842,768 - trpD HEPCGN_25530 0.29 -0.6
3,842,768 - trpD HEPCGN_25530 0.29 -1.0
3,842,846 + trpD HEPCGN_25530 0.34 +1.2
3,842,876 - trpD HEPCGN_25530 0.36 -0.3
3,842,923 - trpD HEPCGN_25530 0.39 +1.0
3,843,039 + trpD HEPCGN_25530 0.46 -0.3
3,843,039 + trpD HEPCGN_25530 0.46 +0.4
3,843,092 + trpD HEPCGN_25530 0.49 +0.5

Or see this region's nucleotide sequence