Strain Fitness in Escherichia coli ECOR38 around HEPCGN_25255

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntqueH and HEPCGN_25250 are separated by 180 nucleotidesHEPCGN_25250 and HEPCGN_25255 are separated by 441 nucleotidesHEPCGN_25255 and rusA are separated by 0 nucleotidesrusA and HEPCGN_25265 overlap by 4 nucleotides HEPCGN_25240: queH - Epoxyqueuosine reductase QueH, at 3,797,102 to 3,797,767 queH HEPCGN_25250: HEPCGN_25250 - protein hokC, at 3,797,948 to 3,798,160 _25250 HEPCGN_25255: HEPCGN_25255 - DUF968 domain-containing protein, at 3,798,602 to 3,799,657 _25255 HEPCGN_25260: rusA - Endodeoxyribonuclease RusA, at 3,799,658 to 3,800,023 rusA HEPCGN_25265: HEPCGN_25265 - antiterminator, at 3,800,020 to 3,800,709 _25265 Position (kb) 3798 3799 3800Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3797.657 kb on + strand, within queHat 3797.673 kb on - strand, within queHat 3797.673 kb on - strand, within queHat 3797.694 kb on + strand, within queHat 3797.695 kb on - strand, within queHat 3797.695 kb on - strand, within queHat 3797.772 kb on - strandat 3797.823 kb on + strandat 3797.823 kb on + strandat 3797.824 kb on - strandat 3797.948 kb on - strandat 3797.948 kb on - strandat 3798.416 kb on - strandat 3798.455 kb on + strandat 3798.466 kb on + strandat 3798.489 kb on + strandat 3798.544 kb on + strandat 3799.047 kb on + strand, within HEPCGN_25255at 3799.048 kb on - strand, within HEPCGN_25255at 3799.048 kb on - strand, within HEPCGN_25255at 3799.083 kb on + strand, within HEPCGN_25255at 3799.083 kb on + strand, within HEPCGN_25255at 3799.084 kb on - strand, within HEPCGN_25255at 3799.098 kb on + strand, within HEPCGN_25255at 3799.152 kb on + strand, within HEPCGN_25255at 3799.153 kb on - strand, within HEPCGN_25255at 3799.153 kb on - strand, within HEPCGN_25255at 3799.216 kb on - strand, within HEPCGN_25255at 3799.355 kb on + strand, within HEPCGN_25255at 3799.396 kb on - strand, within HEPCGN_25255at 3799.396 kb on - strand, within HEPCGN_25255at 3799.396 kb on - strand, within HEPCGN_25255at 3799.398 kb on - strand, within HEPCGN_25255at 3799.517 kb on - strand, within HEPCGN_25255at 3799.532 kb on - strand, within HEPCGN_25255at 3799.532 kb on - strand, within HEPCGN_25255at 3799.655 kb on + strandat 3799.656 kb on - strandat 3799.709 kb on + strand, within rusAat 3799.733 kb on + strand, within rusAat 3799.733 kb on + strand, within rusAat 3799.955 kb on - strand, within rusAat 3800.369 kb on - strand, within HEPCGN_25265at 3800.518 kb on + strand, within HEPCGN_25265at 3800.519 kb on - strand, within HEPCGN_25265at 3800.554 kb on - strand, within HEPCGN_25265at 3800.574 kb on + strand, within HEPCGN_25265at 3800.575 kb on - strand, within HEPCGN_25265at 3800.588 kb on - strand, within HEPCGN_25265at 3800.588 kb on - strand, within HEPCGN_25265

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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3,797,657 + queH HEPCGN_25240 0.83 +1.1
3,797,673 - queH HEPCGN_25240 0.86 -0.4
3,797,673 - queH HEPCGN_25240 0.86 -0.9
3,797,694 + queH HEPCGN_25240 0.89 +2.0
3,797,695 - queH HEPCGN_25240 0.89 +1.3
3,797,695 - queH HEPCGN_25240 0.89 -2.2
3,797,772 - -1.1
3,797,823 + -1.5
3,797,823 + +0.2
3,797,824 - -2.2
3,797,948 - +0.0
3,797,948 - +0.3
3,798,416 - -0.1
3,798,455 + +0.8
3,798,466 + +0.1
3,798,489 + -0.6
3,798,544 + +0.3
3,799,047 + HEPCGN_25255 0.42 +1.5
3,799,048 - HEPCGN_25255 0.42 +0.4
3,799,048 - HEPCGN_25255 0.42 -1.4
3,799,083 + HEPCGN_25255 0.46 +1.0
3,799,083 + HEPCGN_25255 0.46 +1.6
3,799,084 - HEPCGN_25255 0.46 -0.5
3,799,098 + HEPCGN_25255 0.47 +1.9
3,799,152 + HEPCGN_25255 0.52 +0.9
3,799,153 - HEPCGN_25255 0.52 -1.9
3,799,153 - HEPCGN_25255 0.52 -2.3
3,799,216 - HEPCGN_25255 0.58 +0.5
3,799,355 + HEPCGN_25255 0.71 +1.3
3,799,396 - HEPCGN_25255 0.75 +2.2
3,799,396 - HEPCGN_25255 0.75 +3.8
3,799,396 - HEPCGN_25255 0.75 +0.3
3,799,398 - HEPCGN_25255 0.75 -0.7
3,799,517 - HEPCGN_25255 0.87 -1.2
3,799,532 - HEPCGN_25255 0.88 +1.6
3,799,532 - HEPCGN_25255 0.88 +0.5
3,799,655 + +0.8
3,799,656 - +1.7
3,799,709 + rusA HEPCGN_25260 0.14 +1.3
3,799,733 + rusA HEPCGN_25260 0.20 +2.0
3,799,733 + rusA HEPCGN_25260 0.20 -0.1
3,799,955 - rusA HEPCGN_25260 0.81 +1.1
3,800,369 - HEPCGN_25265 0.51 -0.4
3,800,518 + HEPCGN_25265 0.72 +3.0
3,800,519 - HEPCGN_25265 0.72 +1.6
3,800,554 - HEPCGN_25265 0.77 -0.3
3,800,574 + HEPCGN_25265 0.80 +1.0
3,800,575 - HEPCGN_25265 0.80 +0.1
3,800,588 - HEPCGN_25265 0.82 -0.2
3,800,588 - HEPCGN_25265 0.82 -2.0

Or see this region's nucleotide sequence