Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt queH and HEPCGN_25250 are separated by 180 nucleotides HEPCGN_25250 and HEPCGN_25255 are separated by 441 nucleotides HEPCGN_25255 and rusA are separated by 0 nucleotides rusA and HEPCGN_25265 overlap by 4 nucleotides
HEPCGN_25240: queH - Epoxyqueuosine reductase QueH, at 3,797,102 to 3,797,767
queH
HEPCGN_25250: HEPCGN_25250 - protein hokC, at 3,797,948 to 3,798,160
_25250
HEPCGN_25255: HEPCGN_25255 - DUF968 domain-containing protein, at 3,798,602 to 3,799,657
_25255
HEPCGN_25260: rusA - Endodeoxyribonuclease RusA, at 3,799,658 to 3,800,023
rusA
HEPCGN_25265: HEPCGN_25265 - antiterminator, at 3,800,020 to 3,800,709
_25265
Position (kb)
3798
3799
3800 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3797.657 kb on + strand, within queH at 3797.673 kb on - strand, within queH at 3797.673 kb on - strand, within queH at 3797.694 kb on + strand, within queH at 3797.695 kb on - strand, within queH at 3797.695 kb on - strand, within queH at 3797.772 kb on - strand at 3797.823 kb on + strand at 3797.823 kb on + strand at 3797.824 kb on - strand at 3797.948 kb on - strand at 3797.948 kb on - strand at 3798.416 kb on - strand at 3798.455 kb on + strand at 3798.466 kb on + strand at 3798.489 kb on + strand at 3798.544 kb on + strand at 3799.047 kb on + strand, within HEPCGN_25255 at 3799.048 kb on - strand, within HEPCGN_25255 at 3799.048 kb on - strand, within HEPCGN_25255 at 3799.083 kb on + strand, within HEPCGN_25255 at 3799.083 kb on + strand, within HEPCGN_25255 at 3799.084 kb on - strand, within HEPCGN_25255 at 3799.098 kb on + strand, within HEPCGN_25255 at 3799.152 kb on + strand, within HEPCGN_25255 at 3799.153 kb on - strand, within HEPCGN_25255 at 3799.153 kb on - strand, within HEPCGN_25255 at 3799.216 kb on - strand, within HEPCGN_25255 at 3799.355 kb on + strand, within HEPCGN_25255 at 3799.396 kb on - strand, within HEPCGN_25255 at 3799.396 kb on - strand, within HEPCGN_25255 at 3799.396 kb on - strand, within HEPCGN_25255 at 3799.398 kb on - strand, within HEPCGN_25255 at 3799.517 kb on - strand, within HEPCGN_25255 at 3799.532 kb on - strand, within HEPCGN_25255 at 3799.532 kb on - strand, within HEPCGN_25255 at 3799.655 kb on + strand at 3799.656 kb on - strand at 3799.709 kb on + strand, within rusA at 3799.733 kb on + strand, within rusA at 3799.733 kb on + strand, within rusA at 3799.955 kb on - strand, within rusA at 3800.369 kb on - strand, within HEPCGN_25265 at 3800.518 kb on + strand, within HEPCGN_25265 at 3800.519 kb on - strand, within HEPCGN_25265 at 3800.554 kb on - strand, within HEPCGN_25265 at 3800.574 kb on + strand, within HEPCGN_25265 at 3800.575 kb on - strand, within HEPCGN_25265 at 3800.588 kb on - strand, within HEPCGN_25265 at 3800.588 kb on - strand, within HEPCGN_25265
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 3,797,657 + queH HEPCGN_25240 0.83 +1.1 3,797,673 - queH HEPCGN_25240 0.86 -0.4 3,797,673 - queH HEPCGN_25240 0.86 -0.9 3,797,694 + queH HEPCGN_25240 0.89 +2.0 3,797,695 - queH HEPCGN_25240 0.89 +1.3 3,797,695 - queH HEPCGN_25240 0.89 -2.2 3,797,772 - -1.1 3,797,823 + -1.5 3,797,823 + +0.2 3,797,824 - -2.2 3,797,948 - +0.0 3,797,948 - +0.3 3,798,416 - -0.1 3,798,455 + +0.8 3,798,466 + +0.1 3,798,489 + -0.6 3,798,544 + +0.3 3,799,047 + HEPCGN_25255 0.42 +1.5 3,799,048 - HEPCGN_25255 0.42 +0.4 3,799,048 - HEPCGN_25255 0.42 -1.4 3,799,083 + HEPCGN_25255 0.46 +1.0 3,799,083 + HEPCGN_25255 0.46 +1.6 3,799,084 - HEPCGN_25255 0.46 -0.5 3,799,098 + HEPCGN_25255 0.47 +1.9 3,799,152 + HEPCGN_25255 0.52 +0.9 3,799,153 - HEPCGN_25255 0.52 -1.9 3,799,153 - HEPCGN_25255 0.52 -2.3 3,799,216 - HEPCGN_25255 0.58 +0.5 3,799,355 + HEPCGN_25255 0.71 +1.3 3,799,396 - HEPCGN_25255 0.75 +2.2 3,799,396 - HEPCGN_25255 0.75 +3.8 3,799,396 - HEPCGN_25255 0.75 +0.3 3,799,398 - HEPCGN_25255 0.75 -0.7 3,799,517 - HEPCGN_25255 0.87 -1.2 3,799,532 - HEPCGN_25255 0.88 +1.6 3,799,532 - HEPCGN_25255 0.88 +0.5 3,799,655 + +0.8 3,799,656 - +1.7 3,799,709 + rusA HEPCGN_25260 0.14 +1.3 3,799,733 + rusA HEPCGN_25260 0.20 +2.0 3,799,733 + rusA HEPCGN_25260 0.20 -0.1 3,799,955 - rusA HEPCGN_25260 0.81 +1.1 3,800,369 - HEPCGN_25265 0.51 -0.4 3,800,518 + HEPCGN_25265 0.72 +3.0 3,800,519 - HEPCGN_25265 0.72 +1.6 3,800,554 - HEPCGN_25265 0.77 -0.3 3,800,574 + HEPCGN_25265 0.80 +1.0 3,800,575 - HEPCGN_25265 0.80 +0.1 3,800,588 - HEPCGN_25265 0.82 -0.2 3,800,588 - HEPCGN_25265 0.82 -2.0
Or see this region's nucleotide sequence