Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ychT and ychE are separated by 30 nucleotides ychE and HEPCGN_25085 are separated by 26 nucleotides HEPCGN_25085 and HEPCGN_25090 overlap by 25 nucleotides HEPCGN_25090 and oppA are separated by 373 nucleotides
HEPCGN_25075: ychT - Protein YchT, at 3,771,757 to 3,771,810
ychT
HEPCGN_25080: ychE - NAAT family transporter YchE, at 3,771,841 to 3,772,488
ychE
HEPCGN_25085: HEPCGN_25085 - hypothetical protein, at 3,772,515 to 3,772,670
_25085
HEPCGN_25090: HEPCGN_25090 - hypothetical protein, at 3,772,646 to 3,772,807
_25090
HEPCGN_25095: oppA - oligopeptide ABC transporter substrate-binding protein OppA, at 3,773,181 to 3,774,857
oppA
Position (kb)
3772
3773 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3771.647 kb on - strand at 3771.650 kb on + strand at 3771.667 kb on + strand at 3771.671 kb on - strand at 3771.690 kb on - strand at 3771.707 kb on + strand at 3771.708 kb on - strand at 3771.711 kb on + strand at 3771.727 kb on + strand at 3771.737 kb on - strand at 3771.744 kb on + strand at 3771.803 kb on - strand, within ychT at 3771.871 kb on + strand at 3771.921 kb on + strand, within ychE at 3772.008 kb on + strand, within ychE at 3772.008 kb on + strand, within ychE at 3772.060 kb on - strand, within ychE at 3772.062 kb on - strand, within ychE at 3772.109 kb on + strand, within ychE at 3772.248 kb on + strand, within ychE at 3772.263 kb on + strand, within ychE at 3772.281 kb on + strand, within ychE at 3772.281 kb on + strand, within ychE at 3772.281 kb on + strand, within ychE at 3772.281 kb on + strand, within ychE at 3772.282 kb on - strand, within ychE at 3772.404 kb on + strand, within ychE at 3772.407 kb on + strand, within ychE at 3772.408 kb on - strand, within ychE at 3772.415 kb on + strand, within ychE at 3772.486 kb on + strand at 3772.486 kb on + strand at 3772.487 kb on - strand at 3772.525 kb on + strand at 3772.690 kb on + strand, within HEPCGN_25090 at 3772.691 kb on - strand, within HEPCGN_25090 at 3773.023 kb on + strand at 3773.024 kb on - strand at 3773.063 kb on - strand at 3773.063 kb on - strand at 3773.063 kb on - strand at 3773.093 kb on - strand at 3773.107 kb on + strand at 3773.108 kb on - strand at 3773.108 kb on - strand at 3773.110 kb on + strand at 3773.111 kb on - strand at 3773.111 kb on - strand at 3773.125 kb on + strand at 3773.176 kb on + strand at 3773.177 kb on - strand at 3773.198 kb on + strand at 3773.199 kb on - strand at 3773.202 kb on + strand at 3773.254 kb on + strand at 3773.254 kb on + strand at 3773.354 kb on + strand, within oppA at 3773.354 kb on + strand, within oppA at 3773.380 kb on + strand, within oppA at 3773.414 kb on + strand, within oppA at 3773.415 kb on - strand, within oppA at 3773.417 kb on + strand, within oppA at 3773.586 kb on + strand, within oppA at 3773.589 kb on - strand, within oppA at 3773.589 kb on - strand, within oppA at 3773.734 kb on + strand, within oppA at 3773.734 kb on + strand, within oppA at 3773.735 kb on - strand, within oppA at 3773.770 kb on - strand, within oppA at 3773.777 kb on - strand, within oppA at 3773.777 kb on - strand, within oppA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 3,771,647 - -0.6 3,771,650 + +4.0 3,771,667 + -0.8 3,771,671 - +0.2 3,771,690 - -2.0 3,771,707 + +1.0 3,771,708 - +0.3 3,771,711 + -0.3 3,771,727 + -0.4 3,771,737 - -0.9 3,771,744 + +0.0 3,771,803 - ychT HEPCGN_25075 0.85 -0.8 3,771,871 + +0.1 3,771,921 + ychE HEPCGN_25080 0.12 -1.1 3,772,008 + ychE HEPCGN_25080 0.26 +0.9 3,772,008 + ychE HEPCGN_25080 0.26 +1.0 3,772,060 - ychE HEPCGN_25080 0.34 +1.0 3,772,062 - ychE HEPCGN_25080 0.34 +0.1 3,772,109 + ychE HEPCGN_25080 0.41 -0.3 3,772,248 + ychE HEPCGN_25080 0.63 -1.0 3,772,263 + ychE HEPCGN_25080 0.65 -0.3 3,772,281 + ychE HEPCGN_25080 0.68 -0.7 3,772,281 + ychE HEPCGN_25080 0.68 -0.8 3,772,281 + ychE HEPCGN_25080 0.68 +3.0 3,772,281 + ychE HEPCGN_25080 0.68 +0.2 3,772,282 - ychE HEPCGN_25080 0.68 -0.4 3,772,404 + ychE HEPCGN_25080 0.87 -2.3 3,772,407 + ychE HEPCGN_25080 0.87 +0.4 3,772,408 - ychE HEPCGN_25080 0.88 +0.0 3,772,415 + ychE HEPCGN_25080 0.89 +0.2 3,772,486 + +0.3 3,772,486 + +1.1 3,772,487 - -0.8 3,772,525 + -0.8 3,772,690 + HEPCGN_25090 0.27 +3.6 3,772,691 - HEPCGN_25090 0.28 +1.3 3,773,023 + +0.1 3,773,024 - -0.3 3,773,063 - -2.3 3,773,063 - -1.9 3,773,063 - -0.4 3,773,093 - -1.6 3,773,107 + -0.9 3,773,108 - +0.5 3,773,108 - -0.8 3,773,110 + -0.0 3,773,111 - -0.3 3,773,111 - -0.8 3,773,125 + +0.7 3,773,176 + -1.2 3,773,177 - +0.1 3,773,198 + -0.3 3,773,199 - -1.1 3,773,202 + -0.2 3,773,254 + +0.3 3,773,254 + -1.0 3,773,354 + oppA HEPCGN_25095 0.10 +0.2 3,773,354 + oppA HEPCGN_25095 0.10 -3.3 3,773,380 + oppA HEPCGN_25095 0.12 -2.0 3,773,414 + oppA HEPCGN_25095 0.14 -1.6 3,773,415 - oppA HEPCGN_25095 0.14 -2.2 3,773,417 + oppA HEPCGN_25095 0.14 -0.2 3,773,586 + oppA HEPCGN_25095 0.24 -0.9 3,773,589 - oppA HEPCGN_25095 0.24 +1.2 3,773,589 - oppA HEPCGN_25095 0.24 -0.3 3,773,734 + oppA HEPCGN_25095 0.33 -1.2 3,773,734 + oppA HEPCGN_25095 0.33 -1.5 3,773,735 - oppA HEPCGN_25095 0.33 +0.2 3,773,770 - oppA HEPCGN_25095 0.35 +0.4 3,773,777 - oppA HEPCGN_25095 0.36 -0.9 3,773,777 - oppA HEPCGN_25095 0.36 -0.5
Or see this region's nucleotide sequence