Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lolE and nagK are separated by 28 nucleotides nagK and cobB are separated by 15 nucleotides cobB and HEPCGN_24050 are separated by 137 nucleotides HEPCGN_24050 and HEPCGN_24055 overlap by 4 nucleotides
HEPCGN_24035: lolE - lipoprotein-releasing ABC transporter permease subunit LolE, at 3,595,355 to 3,596,593
lolE
HEPCGN_24040: nagK - N-acetylglucosamine kinase, at 3,596,622 to 3,597,533
nagK
HEPCGN_24045: cobB - NAD-dependent protein deacylase, at 3,597,549 to 3,598,370
cobB
HEPCGN_24050: HEPCGN_24050 - Inner membrane protein YcfZ, at 3,598,508 to 3,599,296
_24050
HEPCGN_24055: HEPCGN_24055 - DUF3592 domain-containing protein, at 3,599,293 to 3,599,754
_24055
Position (kb)
3597
3598
3599 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3596.592 kb on - strand at 3596.605 kb on + strand at 3596.606 kb on - strand at 3596.606 kb on - strand at 3596.625 kb on + strand at 3596.625 kb on + strand at 3596.626 kb on - strand at 3596.727 kb on + strand, within nagK at 3596.727 kb on + strand, within nagK at 3596.728 kb on - strand, within nagK at 3596.762 kb on - strand, within nagK at 3596.841 kb on - strand, within nagK at 3596.848 kb on - strand, within nagK at 3596.877 kb on - strand, within nagK at 3596.921 kb on - strand, within nagK at 3597.069 kb on + strand, within nagK at 3597.069 kb on + strand, within nagK at 3597.071 kb on + strand, within nagK at 3597.072 kb on - strand, within nagK at 3597.072 kb on - strand, within nagK at 3597.072 kb on - strand, within nagK at 3597.075 kb on - strand, within nagK at 3597.183 kb on + strand, within nagK at 3597.214 kb on - strand, within nagK at 3597.223 kb on - strand, within nagK at 3597.225 kb on + strand, within nagK at 3597.226 kb on - strand, within nagK at 3597.339 kb on - strand, within nagK at 3597.442 kb on + strand at 3597.443 kb on - strand at 3597.517 kb on + strand at 3597.517 kb on + strand at 3597.518 kb on - strand at 3597.518 kb on - strand at 3597.523 kb on + strand at 3597.524 kb on - strand at 3597.548 kb on + strand at 3597.548 kb on + strand at 3597.641 kb on + strand, within cobB at 3597.717 kb on - strand, within cobB at 3597.822 kb on + strand, within cobB at 3597.977 kb on + strand, within cobB at 3597.984 kb on - strand, within cobB at 3597.984 kb on - strand, within cobB at 3597.984 kb on - strand, within cobB at 3598.109 kb on - strand, within cobB at 3598.109 kb on - strand, within cobB at 3598.149 kb on + strand, within cobB at 3598.150 kb on - strand, within cobB at 3598.191 kb on - strand, within cobB at 3598.206 kb on + strand, within cobB at 3598.368 kb on + strand at 3598.484 kb on + strand at 3598.526 kb on + strand at 3598.526 kb on + strand at 3598.541 kb on - strand at 3598.546 kb on + strand at 3598.611 kb on + strand, within HEPCGN_24050 at 3598.611 kb on + strand, within HEPCGN_24050 at 3598.675 kb on - strand, within HEPCGN_24050 at 3598.800 kb on + strand, within HEPCGN_24050 at 3598.803 kb on + strand, within HEPCGN_24050 at 3598.868 kb on - strand, within HEPCGN_24050 at 3598.937 kb on - strand, within HEPCGN_24050 at 3599.029 kb on + strand, within HEPCGN_24050 at 3599.096 kb on - strand, within HEPCGN_24050 at 3599.116 kb on + strand, within HEPCGN_24050 at 3599.123 kb on + strand, within HEPCGN_24050
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 3,596,592 - -0.3 3,596,605 + -1.7 3,596,606 - +2.9 3,596,606 - +1.5 3,596,625 + +0.4 3,596,625 + +0.9 3,596,626 - +0.8 3,596,727 + nagK HEPCGN_24040 0.12 +0.4 3,596,727 + nagK HEPCGN_24040 0.12 +0.8 3,596,728 - nagK HEPCGN_24040 0.12 -0.6 3,596,762 - nagK HEPCGN_24040 0.15 -0.6 3,596,841 - nagK HEPCGN_24040 0.24 -0.8 3,596,848 - nagK HEPCGN_24040 0.25 +0.2 3,596,877 - nagK HEPCGN_24040 0.28 +0.3 3,596,921 - nagK HEPCGN_24040 0.33 +0.6 3,597,069 + nagK HEPCGN_24040 0.49 +0.3 3,597,069 + nagK HEPCGN_24040 0.49 +0.2 3,597,071 + nagK HEPCGN_24040 0.49 -0.5 3,597,072 - nagK HEPCGN_24040 0.49 +0.4 3,597,072 - nagK HEPCGN_24040 0.49 -1.6 3,597,072 - nagK HEPCGN_24040 0.49 -0.1 3,597,075 - nagK HEPCGN_24040 0.50 -0.2 3,597,183 + nagK HEPCGN_24040 0.62 -0.7 3,597,214 - nagK HEPCGN_24040 0.65 +1.0 3,597,223 - nagK HEPCGN_24040 0.66 -0.2 3,597,225 + nagK HEPCGN_24040 0.66 +0.4 3,597,226 - nagK HEPCGN_24040 0.66 +1.6 3,597,339 - nagK HEPCGN_24040 0.79 -0.4 3,597,442 + +0.6 3,597,443 - +0.7 3,597,517 + +0.3 3,597,517 + -0.1 3,597,518 - +1.0 3,597,518 - -0.2 3,597,523 + +0.3 3,597,524 - +1.1 3,597,548 + +0.3 3,597,548 + -0.3 3,597,641 + cobB HEPCGN_24045 0.11 +1.0 3,597,717 - cobB HEPCGN_24045 0.20 -0.6 3,597,822 + cobB HEPCGN_24045 0.33 +2.6 3,597,977 + cobB HEPCGN_24045 0.52 -0.3 3,597,984 - cobB HEPCGN_24045 0.53 +1.0 3,597,984 - cobB HEPCGN_24045 0.53 +1.4 3,597,984 - cobB HEPCGN_24045 0.53 +0.0 3,598,109 - cobB HEPCGN_24045 0.68 +3.1 3,598,109 - cobB HEPCGN_24045 0.68 +1.2 3,598,149 + cobB HEPCGN_24045 0.73 -1.6 3,598,150 - cobB HEPCGN_24045 0.73 -0.6 3,598,191 - cobB HEPCGN_24045 0.78 -1.1 3,598,206 + cobB HEPCGN_24045 0.80 +0.7 3,598,368 + -0.6 3,598,484 + -0.7 3,598,526 + -0.7 3,598,526 + +0.0 3,598,541 - -0.3 3,598,546 + +0.7 3,598,611 + HEPCGN_24050 0.13 +0.7 3,598,611 + HEPCGN_24050 0.13 +0.8 3,598,675 - HEPCGN_24050 0.21 -0.0 3,598,800 + HEPCGN_24050 0.37 -0.2 3,598,803 + HEPCGN_24050 0.37 -1.0 3,598,868 - HEPCGN_24050 0.46 +0.3 3,598,937 - HEPCGN_24050 0.54 -2.1 3,599,029 + HEPCGN_24050 0.66 -1.6 3,599,096 - HEPCGN_24050 0.75 +0.3 3,599,116 + HEPCGN_24050 0.77 +1.9 3,599,123 + HEPCGN_24050 0.78 +0.5
Or see this region's nucleotide sequence