Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hinT and HEPCGN_23965 are separated by 2 nucleotides HEPCGN_23965 and lpoB are separated by 13 nucleotides lpoB and thiK overlap by 20 nucleotides thiK and nagZ are separated by 10 nucleotides
HEPCGN_23960: hinT - purine nucleoside phosphoramidase, at 3,579,897 to 3,580,256
hinT
HEPCGN_23965: HEPCGN_23965 - DUF1425 domain-containing protein, at 3,580,259 to 3,580,636
_23965
HEPCGN_23970: lpoB - penicillin-binding protein activator LpoB, at 3,580,650 to 3,581,291
lpoB
HEPCGN_23975: thiK - thiamine kinase, at 3,581,272 to 3,582,096
thiK
HEPCGN_23980: nagZ - beta-N-acetylhexosaminidase, at 3,582,107 to 3,583,132
nagZ
Position (kb)
3580
3581
3582 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3579.670 kb on - strand at 3579.753 kb on - strand at 3579.753 kb on - strand at 3579.754 kb on + strand at 3579.757 kb on - strand at 3579.797 kb on - strand at 3579.816 kb on + strand at 3579.855 kb on + strand at 3579.856 kb on - strand at 3579.973 kb on + strand, within hinT at 3580.014 kb on - strand, within hinT at 3580.014 kb on - strand, within hinT at 3580.127 kb on + strand, within hinT at 3580.127 kb on + strand, within hinT at 3580.188 kb on + strand, within hinT at 3580.199 kb on + strand, within hinT at 3580.244 kb on + strand at 3580.451 kb on + strand, within HEPCGN_23965 at 3580.499 kb on + strand, within HEPCGN_23965 at 3580.622 kb on + strand at 3580.623 kb on - strand at 3580.628 kb on + strand at 3580.629 kb on - strand at 3580.634 kb on + strand at 3580.871 kb on + strand, within lpoB at 3580.881 kb on + strand, within lpoB at 3580.881 kb on + strand, within lpoB at 3581.039 kb on + strand, within lpoB at 3581.055 kb on - strand, within lpoB at 3581.173 kb on - strand, within lpoB at 3581.255 kb on + strand at 3581.474 kb on + strand, within thiK at 3581.475 kb on - strand, within thiK at 3581.513 kb on + strand, within thiK at 3581.513 kb on + strand, within thiK at 3581.514 kb on - strand, within thiK at 3581.567 kb on - strand, within thiK at 3581.603 kb on - strand, within thiK at 3581.605 kb on + strand, within thiK at 3581.605 kb on + strand, within thiK at 3581.639 kb on + strand, within thiK at 3581.759 kb on + strand, within thiK at 3581.760 kb on - strand, within thiK at 3581.773 kb on - strand, within thiK at 3581.836 kb on + strand, within thiK at 3581.920 kb on + strand, within thiK at 3581.920 kb on + strand, within thiK at 3581.955 kb on - strand, within thiK at 3582.017 kb on - strand at 3582.080 kb on + strand at 3582.081 kb on - strand at 3582.081 kb on - strand at 3582.095 kb on - strand at 3582.213 kb on - strand, within nagZ at 3582.213 kb on - strand, within nagZ at 3582.232 kb on - strand, within nagZ
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 3,579,670 - +0.2 3,579,753 - +0.7 3,579,753 - -0.4 3,579,754 + -0.5 3,579,757 - -2.0 3,579,797 - -0.1 3,579,816 + -0.7 3,579,855 + +0.3 3,579,856 - +1.2 3,579,973 + hinT HEPCGN_23960 0.21 -0.3 3,580,014 - hinT HEPCGN_23960 0.33 -1.8 3,580,014 - hinT HEPCGN_23960 0.33 +0.8 3,580,127 + hinT HEPCGN_23960 0.64 +1.8 3,580,127 + hinT HEPCGN_23960 0.64 +0.8 3,580,188 + hinT HEPCGN_23960 0.81 +0.4 3,580,199 + hinT HEPCGN_23960 0.84 -2.4 3,580,244 + +0.3 3,580,451 + HEPCGN_23965 0.51 +0.2 3,580,499 + HEPCGN_23965 0.63 -0.1 3,580,622 + +1.6 3,580,623 - +0.0 3,580,628 + +1.7 3,580,629 - +0.0 3,580,634 + +0.6 3,580,871 + lpoB HEPCGN_23970 0.34 +0.7 3,580,881 + lpoB HEPCGN_23970 0.36 +1.5 3,580,881 + lpoB HEPCGN_23970 0.36 +1.8 3,581,039 + lpoB HEPCGN_23970 0.61 +0.5 3,581,055 - lpoB HEPCGN_23970 0.63 +1.5 3,581,173 - lpoB HEPCGN_23970 0.81 +0.9 3,581,255 + +0.5 3,581,474 + thiK HEPCGN_23975 0.24 -0.8 3,581,475 - thiK HEPCGN_23975 0.25 -0.9 3,581,513 + thiK HEPCGN_23975 0.29 +0.1 3,581,513 + thiK HEPCGN_23975 0.29 +1.0 3,581,514 - thiK HEPCGN_23975 0.29 +0.9 3,581,567 - thiK HEPCGN_23975 0.36 -0.2 3,581,603 - thiK HEPCGN_23975 0.40 -1.6 3,581,605 + thiK HEPCGN_23975 0.40 -0.1 3,581,605 + thiK HEPCGN_23975 0.40 -1.7 3,581,639 + thiK HEPCGN_23975 0.44 +2.1 3,581,759 + thiK HEPCGN_23975 0.59 -0.0 3,581,760 - thiK HEPCGN_23975 0.59 +1.6 3,581,773 - thiK HEPCGN_23975 0.61 +1.9 3,581,836 + thiK HEPCGN_23975 0.68 +1.6 3,581,920 + thiK HEPCGN_23975 0.79 -1.0 3,581,920 + thiK HEPCGN_23975 0.79 +0.0 3,581,955 - thiK HEPCGN_23975 0.83 -0.5 3,582,017 - +0.4 3,582,080 + -1.2 3,582,081 - +1.1 3,582,081 - +0.5 3,582,095 - +0.3 3,582,213 - nagZ HEPCGN_23980 0.10 +0.1 3,582,213 - nagZ HEPCGN_23980 0.10 +0.4 3,582,232 - nagZ HEPCGN_23980 0.12 -0.6
Or see this region's nucleotide sequence