Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgaC and pgaB overlap by 8 nucleotides pgaB and HEPCGN_23585 are separated by 33 nucleotides HEPCGN_23585 and pgaA are separated by 8 nucleotides
HEPCGN_23575: pgaC - poly-beta-1,6-N-acetyl-D-glucosamine synthase, at 3,511,469 to 3,512,794
pgaC
HEPCGN_23580: pgaB - poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB, at 3,512,787 to 3,514,658
pgaB
HEPCGN_23585: HEPCGN_23585 - hypothetical protein, at 3,514,692 to 3,514,805
_23585
HEPCGN_23590: pgaA - poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA, at 3,514,814 to 3,517,237
pgaA
Position (kb)
3512
3513
3514
3515 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3511.955 kb on + strand, within pgaC at 3512.018 kb on - strand, within pgaC at 3512.114 kb on + strand, within pgaC at 3512.114 kb on + strand, within pgaC at 3512.114 kb on + strand, within pgaC at 3512.128 kb on + strand, within pgaC at 3512.129 kb on - strand, within pgaC at 3512.502 kb on - strand, within pgaC at 3512.566 kb on + strand, within pgaC at 3512.666 kb on - strand at 3512.728 kb on - strand at 3512.762 kb on + strand at 3512.831 kb on + strand at 3513.421 kb on - strand, within pgaB at 3513.459 kb on + strand, within pgaB at 3513.460 kb on - strand, within pgaB at 3513.460 kb on - strand, within pgaB at 3513.557 kb on - strand, within pgaB at 3513.594 kb on + strand, within pgaB at 3513.594 kb on + strand, within pgaB at 3513.613 kb on + strand, within pgaB at 3513.613 kb on + strand, within pgaB at 3513.891 kb on - strand, within pgaB at 3513.936 kb on + strand, within pgaB at 3514.181 kb on - strand, within pgaB at 3514.192 kb on - strand, within pgaB at 3514.196 kb on - strand, within pgaB at 3514.198 kb on - strand, within pgaB at 3514.535 kb on + strand at 3514.607 kb on + strand at 3514.716 kb on + strand, within HEPCGN_23585 at 3514.719 kb on - strand, within HEPCGN_23585 at 3514.762 kb on - strand, within HEPCGN_23585 at 3514.845 kb on + strand at 3514.846 kb on - strand at 3514.873 kb on - strand at 3514.930 kb on + strand at 3514.930 kb on + strand at 3515.035 kb on + strand at 3515.261 kb on - strand, within pgaA at 3515.261 kb on - strand, within pgaA at 3515.289 kb on + strand, within pgaA at 3515.290 kb on - strand, within pgaA at 3515.290 kb on - strand, within pgaA at 3515.383 kb on + strand, within pgaA at 3515.383 kb on + strand, within pgaA at 3515.389 kb on + strand, within pgaA at 3515.474 kb on - strand, within pgaA at 3515.534 kb on - strand, within pgaA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 3,511,955 + pgaC HEPCGN_23575 0.37 +1.5 3,512,018 - pgaC HEPCGN_23575 0.41 -0.3 3,512,114 + pgaC HEPCGN_23575 0.49 -0.8 3,512,114 + pgaC HEPCGN_23575 0.49 -0.8 3,512,114 + pgaC HEPCGN_23575 0.49 +0.6 3,512,128 + pgaC HEPCGN_23575 0.50 -0.3 3,512,129 - pgaC HEPCGN_23575 0.50 -0.9 3,512,502 - pgaC HEPCGN_23575 0.78 -1.1 3,512,566 + pgaC HEPCGN_23575 0.83 +0.5 3,512,666 - -1.5 3,512,728 - -1.7 3,512,762 + +4.4 3,512,831 + +0.7 3,513,421 - pgaB HEPCGN_23580 0.34 +1.6 3,513,459 + pgaB HEPCGN_23580 0.36 +0.6 3,513,460 - pgaB HEPCGN_23580 0.36 +0.8 3,513,460 - pgaB HEPCGN_23580 0.36 -0.8 3,513,557 - pgaB HEPCGN_23580 0.41 -1.7 3,513,594 + pgaB HEPCGN_23580 0.43 +0.5 3,513,594 + pgaB HEPCGN_23580 0.43 +0.7 3,513,613 + pgaB HEPCGN_23580 0.44 -0.9 3,513,613 + pgaB HEPCGN_23580 0.44 -0.2 3,513,891 - pgaB HEPCGN_23580 0.59 +1.4 3,513,936 + pgaB HEPCGN_23580 0.61 +0.2 3,514,181 - pgaB HEPCGN_23580 0.74 +2.6 3,514,192 - pgaB HEPCGN_23580 0.75 +0.0 3,514,196 - pgaB HEPCGN_23580 0.75 -1.8 3,514,198 - pgaB HEPCGN_23580 0.75 +1.0 3,514,535 + +0.6 3,514,607 + -0.5 3,514,716 + HEPCGN_23585 0.21 -0.2 3,514,719 - HEPCGN_23585 0.24 +0.9 3,514,762 - HEPCGN_23585 0.61 -0.1 3,514,845 + -1.1 3,514,846 - +0.5 3,514,873 - -0.7 3,514,930 + -1.1 3,514,930 + -0.6 3,515,035 + -1.1 3,515,261 - pgaA HEPCGN_23590 0.18 -0.3 3,515,261 - pgaA HEPCGN_23590 0.18 +0.4 3,515,289 + pgaA HEPCGN_23590 0.20 +0.6 3,515,290 - pgaA HEPCGN_23590 0.20 +0.4 3,515,290 - pgaA HEPCGN_23590 0.20 -2.7 3,515,383 + pgaA HEPCGN_23590 0.23 -0.1 3,515,383 + pgaA HEPCGN_23590 0.23 +0.1 3,515,389 + pgaA HEPCGN_23590 0.24 -1.4 3,515,474 - pgaA HEPCGN_23590 0.27 +0.6 3,515,534 - pgaA HEPCGN_23590 0.30 -0.1
Or see this region's nucleotide sequence