Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yejO and Pro_trna are separated by 100 nucleotides Pro_trna and yejM are separated by 74 nucleotides yejM and yejL are separated by 19 nucleotides yejL and yejK are separated by 181 nucleotides
HEPCGN_22590: yejO - autotransporter YejO, at 3,287,539 to 3,290,130
yejO
HEPCGN_22595: Pro_trna - tRNA-Pro(ggg), at 3,290,231 to 3,290,307
_trna
HEPCGN_22600: yejM - LPS biosynthesis-modulating metalloenzyme YejM, at 3,290,382 to 3,292,142
yejM
HEPCGN_22605: yejL - UPF0352 protein YejL, at 3,292,162 to 3,292,389
yejL
HEPCGN_22610: yejK - nucleoid-associated protein YejK, at 3,292,571 to 3,293,578
yejK
Position (kb)
3290
3291
3292
3293 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3289.543 kb on + strand, within yejO at 3289.543 kb on + strand, within yejO at 3289.544 kb on - strand, within yejO at 3289.544 kb on - strand, within yejO at 3289.544 kb on - strand, within yejO at 3289.558 kb on + strand, within yejO at 3289.673 kb on - strand, within yejO at 3289.677 kb on + strand, within yejO at 3289.678 kb on - strand, within yejO at 3289.678 kb on - strand, within yejO at 3289.747 kb on + strand, within yejO at 3289.747 kb on + strand, within yejO at 3289.748 kb on - strand, within yejO at 3289.787 kb on - strand, within yejO at 3289.833 kb on - strand, within yejO at 3289.974 kb on + strand at 3290.065 kb on + strand at 3290.066 kb on - strand at 3290.084 kb on + strand at 3290.142 kb on - strand at 3290.142 kb on - strand at 3290.179 kb on + strand at 3290.179 kb on + strand at 3290.300 kb on - strand at 3290.321 kb on - strand at 3290.356 kb on + strand at 3290.416 kb on - strand at 3290.416 kb on - strand at 3290.470 kb on - strand at 3290.476 kb on + strand at 3290.495 kb on + strand at 3290.495 kb on + strand at 3290.528 kb on + strand at 3290.529 kb on - strand at 3290.635 kb on - strand, within yejM at 3290.688 kb on - strand, within yejM at 3290.804 kb on + strand, within yejM at 3290.897 kb on - strand, within yejM at 3290.973 kb on - strand, within yejM at 3291.038 kb on + strand, within yejM at 3291.043 kb on - strand, within yejM at 3291.191 kb on + strand, within yejM at 3291.193 kb on + strand, within yejM at 3291.194 kb on - strand, within yejM at 3291.329 kb on - strand, within yejM at 3291.348 kb on - strand, within yejM at 3291.457 kb on + strand, within yejM at 3292.196 kb on - strand, within yejL at 3292.196 kb on - strand, within yejL at 3292.390 kb on - strand at 3292.423 kb on - strand at 3292.423 kb on - strand at 3292.423 kb on - strand at 3292.423 kb on - strand at 3292.425 kb on - strand at 3292.425 kb on - strand at 3292.466 kb on - strand at 3292.512 kb on + strand at 3292.556 kb on - strand at 3292.567 kb on + strand at 3292.568 kb on - strand at 3292.582 kb on + strand at 3292.710 kb on - strand, within yejK at 3292.710 kb on - strand, within yejK at 3292.710 kb on - strand, within yejK at 3292.710 kb on - strand, within yejK at 3292.730 kb on + strand, within yejK at 3292.846 kb on - strand, within yejK at 3292.898 kb on + strand, within yejK at 3292.913 kb on + strand, within yejK at 3293.011 kb on + strand, within yejK at 3293.031 kb on + strand, within yejK
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 3,289,543 + yejO HEPCGN_22590 0.77 +1.0 3,289,543 + yejO HEPCGN_22590 0.77 -0.4 3,289,544 - yejO HEPCGN_22590 0.77 +0.2 3,289,544 - yejO HEPCGN_22590 0.77 -0.0 3,289,544 - yejO HEPCGN_22590 0.77 -0.0 3,289,558 + yejO HEPCGN_22590 0.78 -0.1 3,289,673 - yejO HEPCGN_22590 0.82 +0.6 3,289,677 + yejO HEPCGN_22590 0.82 +0.5 3,289,678 - yejO HEPCGN_22590 0.83 -1.8 3,289,678 - yejO HEPCGN_22590 0.83 +0.3 3,289,747 + yejO HEPCGN_22590 0.85 +0.0 3,289,747 + yejO HEPCGN_22590 0.85 -0.1 3,289,748 - yejO HEPCGN_22590 0.85 +0.6 3,289,787 - yejO HEPCGN_22590 0.87 +0.2 3,289,833 - yejO HEPCGN_22590 0.89 -1.3 3,289,974 + +1.2 3,290,065 + +0.0 3,290,066 - -0.5 3,290,084 + -0.7 3,290,142 - +0.8 3,290,142 - +0.6 3,290,179 + +0.8 3,290,179 + +0.8 3,290,300 - -0.5 3,290,321 - -1.2 3,290,356 + +3.2 3,290,416 - -0.2 3,290,416 - -3.2 3,290,470 - +2.5 3,290,476 + +0.6 3,290,495 + +0.2 3,290,495 + -1.3 3,290,528 + -0.8 3,290,529 - +1.5 3,290,635 - yejM HEPCGN_22600 0.14 -2.4 3,290,688 - yejM HEPCGN_22600 0.17 +0.2 3,290,804 + yejM HEPCGN_22600 0.24 +2.0 3,290,897 - yejM HEPCGN_22600 0.29 +1.1 3,290,973 - yejM HEPCGN_22600 0.34 -0.6 3,291,038 + yejM HEPCGN_22600 0.37 +1.8 3,291,043 - yejM HEPCGN_22600 0.38 +3.5 3,291,191 + yejM HEPCGN_22600 0.46 -1.7 3,291,193 + yejM HEPCGN_22600 0.46 -0.1 3,291,194 - yejM HEPCGN_22600 0.46 -1.2 3,291,329 - yejM HEPCGN_22600 0.54 +1.9 3,291,348 - yejM HEPCGN_22600 0.55 -1.4 3,291,457 + yejM HEPCGN_22600 0.61 -1.3 3,292,196 - yejL HEPCGN_22605 0.15 -1.3 3,292,196 - yejL HEPCGN_22605 0.15 -1.8 3,292,390 - +0.4 3,292,423 - +1.5 3,292,423 - +1.8 3,292,423 - -0.9 3,292,423 - -0.9 3,292,425 - -0.8 3,292,425 - -1.1 3,292,466 - -0.2 3,292,512 + -0.9 3,292,556 - -1.2 3,292,567 + +3.2 3,292,568 - -0.0 3,292,582 + +0.4 3,292,710 - yejK HEPCGN_22610 0.14 +0.5 3,292,710 - yejK HEPCGN_22610 0.14 -0.2 3,292,710 - yejK HEPCGN_22610 0.14 +0.5 3,292,710 - yejK HEPCGN_22610 0.14 -1.5 3,292,730 + yejK HEPCGN_22610 0.16 -1.2 3,292,846 - yejK HEPCGN_22610 0.27 -0.4 3,292,898 + yejK HEPCGN_22610 0.32 -0.3 3,292,913 + yejK HEPCGN_22610 0.34 -0.4 3,293,011 + yejK HEPCGN_22610 0.44 +1.3 3,293,031 + yejK HEPCGN_22610 0.46 +0.1
Or see this region's nucleotide sequence