Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eco and yojO are separated by 134 nucleotides yojO and HEPCGN_22505 are separated by 51 nucleotides HEPCGN_22505 and napF are separated by 15 nucleotides napF and napD overlap by 11 nucleotides napD and napA overlap by 4 nucleotides
HEPCGN_22495: eco - serine protease inhibitor ecotin, at 3,273,502 to 3,273,990
eco
HEPCGN_22500: yojO - Uncharacterized protein YojO, at 3,274,125 to 3,274,289
yojO
HEPCGN_22505: HEPCGN_22505 - Orf 5, at 3,274,341 to 3,274,382
_22505
HEPCGN_22510: napF - ferredoxin-type protein NapF, at 3,274,398 to 3,274,892
napF
HEPCGN_22515: napD - chaperone NapD, at 3,274,882 to 3,275,145
napD
HEPCGN_22520: napA - nitrate reductase catalytic subunit NapA, at 3,275,142 to 3,277,628
napA
Position (kb)
3274
3275 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3273.400 kb on - strand at 3273.498 kb on + strand at 3273.498 kb on + strand at 3273.499 kb on - strand at 3273.499 kb on - strand at 3273.551 kb on + strand, within eco at 3273.577 kb on - strand, within eco at 3273.603 kb on + strand, within eco at 3273.606 kb on - strand, within eco at 3273.717 kb on - strand, within eco at 3273.720 kb on - strand, within eco at 3273.809 kb on - strand, within eco at 3273.817 kb on + strand, within eco at 3273.829 kb on - strand, within eco at 3273.893 kb on + strand, within eco at 3273.893 kb on + strand, within eco at 3273.894 kb on - strand, within eco at 3273.976 kb on + strand at 3273.977 kb on - strand at 3274.051 kb on - strand at 3274.082 kb on + strand at 3274.082 kb on + strand at 3274.083 kb on - strand at 3274.155 kb on - strand, within yojO at 3274.155 kb on - strand, within yojO at 3274.159 kb on - strand, within yojO at 3274.269 kb on + strand, within yojO at 3274.305 kb on + strand at 3274.309 kb on - strand at 3274.380 kb on + strand at 3274.381 kb on - strand at 3274.384 kb on + strand at 3274.385 kb on - strand at 3274.427 kb on - strand at 3274.604 kb on + strand, within napF at 3274.764 kb on - strand, within napF at 3274.805 kb on + strand, within napF at 3274.806 kb on - strand, within napF at 3274.864 kb on + strand at 3274.865 kb on - strand at 3274.865 kb on - strand at 3274.875 kb on + strand at 3274.890 kb on + strand at 3274.890 kb on + strand at 3274.890 kb on + strand at 3275.069 kb on + strand, within napD at 3275.070 kb on - strand, within napD at 3275.258 kb on + strand at 3275.387 kb on + strand at 3275.394 kb on + strand, within napA at 3275.451 kb on - strand, within napA at 3275.463 kb on - strand, within napA at 3275.467 kb on - strand, within napA at 3275.467 kb on - strand, within napA at 3275.573 kb on + strand, within napA at 3275.610 kb on + strand, within napA at 3275.678 kb on + strand, within napA at 3275.679 kb on - strand, within napA at 3275.708 kb on + strand, within napA at 3275.870 kb on + strand, within napA at 3275.875 kb on - strand, within napA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 3,273,400 - -0.5 3,273,498 + -1.0 3,273,498 + +1.3 3,273,499 - +0.3 3,273,499 - +0.3 3,273,551 + eco HEPCGN_22495 0.10 +1.4 3,273,577 - eco HEPCGN_22495 0.15 +0.0 3,273,603 + eco HEPCGN_22495 0.21 +2.0 3,273,606 - eco HEPCGN_22495 0.21 -1.4 3,273,717 - eco HEPCGN_22495 0.44 +1.2 3,273,720 - eco HEPCGN_22495 0.45 -0.2 3,273,809 - eco HEPCGN_22495 0.63 -0.4 3,273,817 + eco HEPCGN_22495 0.64 +2.2 3,273,829 - eco HEPCGN_22495 0.67 -2.8 3,273,893 + eco HEPCGN_22495 0.80 -1.1 3,273,893 + eco HEPCGN_22495 0.80 +0.3 3,273,894 - eco HEPCGN_22495 0.80 +0.5 3,273,976 + -1.0 3,273,977 - -1.5 3,274,051 - +0.6 3,274,082 + -0.9 3,274,082 + +0.1 3,274,083 - +0.6 3,274,155 - yojO HEPCGN_22500 0.18 +1.3 3,274,155 - yojO HEPCGN_22500 0.18 -0.8 3,274,159 - yojO HEPCGN_22500 0.21 -0.2 3,274,269 + yojO HEPCGN_22500 0.87 +0.9 3,274,305 + +1.1 3,274,309 - +0.8 3,274,380 + +0.9 3,274,381 - -2.3 3,274,384 + -0.4 3,274,385 - -0.0 3,274,427 - -0.3 3,274,604 + napF HEPCGN_22510 0.42 -0.8 3,274,764 - napF HEPCGN_22510 0.74 +3.6 3,274,805 + napF HEPCGN_22510 0.82 +0.2 3,274,806 - napF HEPCGN_22510 0.82 +0.3 3,274,864 + +0.2 3,274,865 - +0.9 3,274,865 - +0.2 3,274,875 + -0.7 3,274,890 + -0.3 3,274,890 + -0.5 3,274,890 + -0.5 3,275,069 + napD HEPCGN_22515 0.71 +0.5 3,275,070 - napD HEPCGN_22515 0.71 +0.9 3,275,258 + +0.4 3,275,387 + +1.5 3,275,394 + napA HEPCGN_22520 0.10 +1.0 3,275,451 - napA HEPCGN_22520 0.12 +2.3 3,275,463 - napA HEPCGN_22520 0.13 -1.5 3,275,467 - napA HEPCGN_22520 0.13 +0.1 3,275,467 - napA HEPCGN_22520 0.13 +0.2 3,275,573 + napA HEPCGN_22520 0.17 -1.7 3,275,610 + napA HEPCGN_22520 0.19 +3.3 3,275,678 + napA HEPCGN_22520 0.22 -0.7 3,275,679 - napA HEPCGN_22520 0.22 -1.5 3,275,708 + napA HEPCGN_22520 0.23 +2.2 3,275,870 + napA HEPCGN_22520 0.29 -1.4 3,275,875 - napA HEPCGN_22520 0.29 +0.0
Or see this region's nucleotide sequence