Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoH and nuoI are separated by 14 nucleotides nuoI and nuoJ are separated by 11 nucleotides nuoJ and nuoK overlap by 4 nucleotides nuoK and nuoL overlap by 4 nucleotides
HEPCGN_22075: nuoH - NADH-quinone oxidoreductase subunit NuoH, at 3,173,014 to 3,173,991
nuoH
HEPCGN_22080: nuoI - NADH-quinone oxidoreductase subunit NuoI, at 3,174,006 to 3,174,548
nuoI
HEPCGN_22085: nuoJ - NADH-quinone oxidoreductase subunit J, at 3,174,560 to 3,175,114
nuoJ
HEPCGN_22090: nuoK - NADH-quinone oxidoreductase subunit NuoK, at 3,175,111 to 3,175,413
nuoK
HEPCGN_22095: nuoL - NADH-quinone oxidoreductase subunit L, at 3,175,410 to 3,177,251
nuoL
Position (kb)
3174
3175
3176 Strain fitness (log2 ratio)
-2
-1
0
1 at 3173.848 kb on - strand, within nuoH at 3173.903 kb on + strand at 3174.014 kb on - strand at 3174.028 kb on + strand at 3174.050 kb on + strand at 3174.051 kb on - strand at 3174.051 kb on - strand at 3174.053 kb on + strand at 3174.054 kb on - strand at 3174.054 kb on - strand at 3174.146 kb on + strand, within nuoI at 3174.146 kb on + strand, within nuoI at 3174.195 kb on - strand, within nuoI at 3174.378 kb on + strand, within nuoI at 3174.450 kb on - strand, within nuoI at 3174.470 kb on + strand, within nuoI at 3174.471 kb on - strand, within nuoI at 3174.471 kb on - strand, within nuoI at 3174.492 kb on - strand, within nuoI at 3174.575 kb on + strand at 3174.576 kb on - strand at 3174.577 kb on + strand at 3174.597 kb on + strand at 3174.598 kb on - strand at 3174.598 kb on - strand at 3174.705 kb on - strand, within nuoJ at 3174.748 kb on + strand, within nuoJ at 3174.938 kb on - strand, within nuoJ at 3174.952 kb on + strand, within nuoJ at 3174.953 kb on - strand, within nuoJ at 3174.969 kb on - strand, within nuoJ at 3174.973 kb on - strand, within nuoJ at 3174.994 kb on + strand, within nuoJ at 3174.994 kb on + strand, within nuoJ at 3175.151 kb on + strand, within nuoK at 3175.264 kb on + strand, within nuoK at 3175.295 kb on - strand, within nuoK at 3175.394 kb on + strand at 3175.434 kb on - strand at 3175.522 kb on + strand at 3175.540 kb on + strand at 3175.670 kb on + strand, within nuoL at 3175.713 kb on + strand, within nuoL at 3175.713 kb on + strand, within nuoL at 3175.713 kb on + strand, within nuoL at 3175.714 kb on - strand, within nuoL at 3175.714 kb on - strand, within nuoL at 3175.714 kb on - strand, within nuoL at 3175.725 kb on + strand, within nuoL at 3175.860 kb on + strand, within nuoL at 3175.878 kb on + strand, within nuoL
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 3,173,848 - nuoH HEPCGN_22075 0.85 -0.0 3,173,903 + +0.6 3,174,014 - +1.7 3,174,028 + +0.6 3,174,050 + -1.9 3,174,051 - -1.3 3,174,051 - -1.4 3,174,053 + -1.9 3,174,054 - -1.2 3,174,054 - -1.8 3,174,146 + nuoI HEPCGN_22080 0.26 -0.0 3,174,146 + nuoI HEPCGN_22080 0.26 -1.8 3,174,195 - nuoI HEPCGN_22080 0.35 -0.3 3,174,378 + nuoI HEPCGN_22080 0.69 -1.3 3,174,450 - nuoI HEPCGN_22080 0.82 -1.0 3,174,470 + nuoI HEPCGN_22080 0.85 -1.7 3,174,471 - nuoI HEPCGN_22080 0.86 -0.2 3,174,471 - nuoI HEPCGN_22080 0.86 -2.3 3,174,492 - nuoI HEPCGN_22080 0.90 -0.3 3,174,575 + -1.6 3,174,576 - -2.0 3,174,577 + -2.6 3,174,597 + -2.2 3,174,598 - -1.4 3,174,598 - -2.3 3,174,705 - nuoJ HEPCGN_22085 0.26 +0.2 3,174,748 + nuoJ HEPCGN_22085 0.34 -2.7 3,174,938 - nuoJ HEPCGN_22085 0.68 -0.4 3,174,952 + nuoJ HEPCGN_22085 0.71 -1.1 3,174,953 - nuoJ HEPCGN_22085 0.71 -1.4 3,174,969 - nuoJ HEPCGN_22085 0.74 +1.7 3,174,973 - nuoJ HEPCGN_22085 0.74 -1.0 3,174,994 + nuoJ HEPCGN_22085 0.78 +1.0 3,174,994 + nuoJ HEPCGN_22085 0.78 -0.6 3,175,151 + nuoK HEPCGN_22090 0.13 -0.3 3,175,264 + nuoK HEPCGN_22090 0.50 -2.5 3,175,295 - nuoK HEPCGN_22090 0.61 -0.5 3,175,394 + -0.9 3,175,434 - +0.7 3,175,522 + -2.4 3,175,540 + -0.3 3,175,670 + nuoL HEPCGN_22095 0.14 -1.0 3,175,713 + nuoL HEPCGN_22095 0.16 -1.6 3,175,713 + nuoL HEPCGN_22095 0.16 -1.7 3,175,713 + nuoL HEPCGN_22095 0.16 -2.6 3,175,714 - nuoL HEPCGN_22095 0.17 +0.3 3,175,714 - nuoL HEPCGN_22095 0.17 -1.0 3,175,714 - nuoL HEPCGN_22095 0.17 -1.6 3,175,725 + nuoL HEPCGN_22095 0.17 -0.7 3,175,860 + nuoL HEPCGN_22095 0.24 -2.2 3,175,878 + nuoL HEPCGN_22095 0.25 +0.7
Or see this region's nucleotide sequence