Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_21360 and crr are separated by 47 nucleotides crr and ptsI are separated by 40 nucleotides ptsI and ptsH are separated by 44 nucleotides ptsH and cysK are separated by 383 nucleotides
HEPCGN_21360: HEPCGN_21360 - IS4-like element IS4 family transposase, at 3,024,354 to 3,025,682
_21360
HEPCGN_21365: crr - PTS glucose transporter subunit IIA, at 3,025,730 to 3,026,239
crr
HEPCGN_21370: ptsI - phosphoenolpyruvate-protein phosphotransferase PtsI, at 3,026,280 to 3,028,007
ptsI
HEPCGN_21375: ptsH - phosphocarrier protein Hpr, at 3,028,052 to 3,028,309
ptsH
HEPCGN_21380: cysK - cysteine synthase A, at 3,028,693 to 3,029,664
cysK
Position (kb)
3026
3027
3028
3029 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3025.680 kb on + strand at 3025.694 kb on + strand at 3025.819 kb on + strand, within crr at 3025.820 kb on - strand, within crr at 3025.820 kb on - strand, within crr at 3026.049 kb on - strand, within crr at 3026.180 kb on - strand, within crr at 3026.251 kb on - strand at 3026.393 kb on + strand at 3026.408 kb on + strand at 3026.474 kb on + strand, within ptsI at 3026.474 kb on + strand, within ptsI at 3026.544 kb on - strand, within ptsI at 3026.843 kb on + strand, within ptsI at 3026.844 kb on - strand, within ptsI at 3026.977 kb on + strand, within ptsI at 3026.977 kb on + strand, within ptsI at 3026.978 kb on - strand, within ptsI at 3027.120 kb on - strand, within ptsI at 3027.120 kb on - strand, within ptsI at 3027.124 kb on + strand, within ptsI at 3027.189 kb on - strand, within ptsI at 3027.359 kb on - strand, within ptsI at 3027.435 kb on - strand, within ptsI at 3027.619 kb on - strand, within ptsI at 3027.722 kb on + strand, within ptsI at 3027.776 kb on + strand, within ptsI at 3027.776 kb on + strand, within ptsI at 3027.780 kb on - strand, within ptsI at 3028.018 kb on + strand at 3028.312 kb on + strand at 3028.326 kb on - strand at 3028.326 kb on - strand at 3028.328 kb on - strand at 3028.333 kb on + strand at 3028.354 kb on + strand at 3028.381 kb on - strand at 3028.483 kb on - strand at 3028.483 kb on - strand at 3028.694 kb on + strand at 3028.695 kb on - strand at 3028.745 kb on - strand at 3028.814 kb on - strand, within cysK at 3028.814 kb on - strand, within cysK at 3028.852 kb on - strand, within cysK at 3028.852 kb on - strand, within cysK at 3028.863 kb on + strand, within cysK
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 3,025,680 + +0.4 3,025,694 + -0.1 3,025,819 + crr HEPCGN_21365 0.17 -1.7 3,025,820 - crr HEPCGN_21365 0.18 +0.2 3,025,820 - crr HEPCGN_21365 0.18 -0.2 3,026,049 - crr HEPCGN_21365 0.63 -2.1 3,026,180 - crr HEPCGN_21365 0.88 -1.1 3,026,251 - -0.1 3,026,393 + +0.5 3,026,408 + -0.1 3,026,474 + ptsI HEPCGN_21370 0.11 -0.2 3,026,474 + ptsI HEPCGN_21370 0.11 +1.3 3,026,544 - ptsI HEPCGN_21370 0.15 +3.3 3,026,843 + ptsI HEPCGN_21370 0.33 +0.8 3,026,844 - ptsI HEPCGN_21370 0.33 -0.2 3,026,977 + ptsI HEPCGN_21370 0.40 -0.4 3,026,977 + ptsI HEPCGN_21370 0.40 +1.0 3,026,978 - ptsI HEPCGN_21370 0.40 +0.5 3,027,120 - ptsI HEPCGN_21370 0.49 -1.4 3,027,120 - ptsI HEPCGN_21370 0.49 -2.1 3,027,124 + ptsI HEPCGN_21370 0.49 -0.2 3,027,189 - ptsI HEPCGN_21370 0.53 +0.8 3,027,359 - ptsI HEPCGN_21370 0.62 +0.2 3,027,435 - ptsI HEPCGN_21370 0.67 +0.0 3,027,619 - ptsI HEPCGN_21370 0.77 -0.1 3,027,722 + ptsI HEPCGN_21370 0.83 +0.3 3,027,776 + ptsI HEPCGN_21370 0.87 -0.7 3,027,776 + ptsI HEPCGN_21370 0.87 +0.5 3,027,780 - ptsI HEPCGN_21370 0.87 -0.1 3,028,018 + +0.3 3,028,312 + +0.3 3,028,326 - -0.2 3,028,326 - -0.4 3,028,328 - -0.5 3,028,333 + +1.3 3,028,354 + +1.3 3,028,381 - -1.7 3,028,483 - -0.3 3,028,483 - -1.1 3,028,694 + +1.5 3,028,695 - -1.2 3,028,745 - -0.9 3,028,814 - cysK HEPCGN_21380 0.12 +0.5 3,028,814 - cysK HEPCGN_21380 0.12 +0.7 3,028,852 - cysK HEPCGN_21380 0.16 -1.5 3,028,852 - cysK HEPCGN_21380 0.16 -0.2 3,028,863 + cysK HEPCGN_21380 0.17 +0.1
Or see this region's nucleotide sequence