Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppk1 and purN are separated by 171 nucleotides purN and purM overlap by 1 nucleotides purM and upp are separated by 324 nucleotides
HEPCGN_20965: ppk1 - polyphosphate kinase 1, at 2,943,738 to 2,945,804
ppk1
HEPCGN_20970: purN - phosphoribosylglycinamide formyltransferase, at 2,945,976 to 2,946,614
purN
HEPCGN_20975: purM - Phosphoribosylformylglycinamidine cyclo-ligase, at 2,946,614 to 2,947,651
purM
HEPCGN_20980: upp - uracil phosphoribosyltransferase, at 2,947,976 to 2,948,602
upp
Position (kb)
2946
2947
2948 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2945.618 kb on + strand at 2945.618 kb on + strand at 2945.657 kb on - strand at 2945.657 kb on - strand at 2945.681 kb on - strand at 2945.785 kb on + strand at 2945.785 kb on + strand at 2945.786 kb on - strand at 2945.787 kb on + strand at 2945.787 kb on + strand at 2945.787 kb on + strand at 2945.787 kb on + strand at 2945.805 kb on + strand at 2945.850 kb on - strand at 2945.861 kb on + strand at 2945.879 kb on + strand at 2945.879 kb on + strand at 2945.880 kb on - strand at 2946.089 kb on + strand, within purN at 2946.148 kb on - strand, within purN at 2946.166 kb on - strand, within purN at 2946.171 kb on - strand, within purN at 2946.216 kb on - strand, within purN at 2946.382 kb on + strand, within purN at 2946.383 kb on - strand, within purN at 2946.383 kb on - strand, within purN at 2946.383 kb on - strand, within purN at 2946.415 kb on + strand, within purN at 2946.415 kb on + strand, within purN at 2946.452 kb on + strand, within purN at 2946.453 kb on - strand, within purN at 2946.537 kb on - strand, within purN at 2946.563 kb on + strand at 2946.563 kb on + strand at 2946.578 kb on + strand at 2946.596 kb on + strand at 2946.596 kb on + strand at 2946.608 kb on + strand at 2946.736 kb on + strand, within purM at 2946.737 kb on - strand, within purM at 2946.966 kb on + strand, within purM at 2946.967 kb on - strand, within purM at 2947.191 kb on + strand, within purM at 2947.192 kb on - strand, within purM at 2947.206 kb on + strand, within purM at 2947.206 kb on + strand, within purM at 2947.206 kb on + strand, within purM at 2947.329 kb on + strand, within purM at 2947.330 kb on - strand, within purM at 2947.421 kb on + strand, within purM at 2947.448 kb on - strand, within purM at 2947.566 kb on - strand at 2947.566 kb on - strand at 2947.594 kb on + strand at 2947.626 kb on + strand at 2947.630 kb on + strand at 2947.631 kb on - strand at 2947.631 kb on - strand at 2947.672 kb on - strand at 2947.707 kb on - strand at 2947.786 kb on + strand at 2947.793 kb on - strand at 2947.928 kb on + strand at 2947.928 kb on + strand at 2947.930 kb on + strand at 2947.931 kb on - strand at 2947.975 kb on + strand at 2947.976 kb on - strand at 2948.042 kb on - strand, within upp at 2948.096 kb on + strand, within upp at 2948.097 kb on - strand, within upp at 2948.179 kb on + strand, within upp at 2948.280 kb on - strand, within upp at 2948.348 kb on - strand, within upp at 2948.510 kb on + strand, within upp at 2948.510 kb on + strand, within upp at 2948.511 kb on - strand, within upp at 2948.511 kb on - strand, within upp at 2948.511 kb on - strand, within upp at 2948.512 kb on + strand, within upp at 2948.550 kb on - strand at 2948.554 kb on + strand at 2948.554 kb on + strand at 2948.554 kb on + strand at 2948.617 kb on + strand at 2948.618 kb on - strand at 2948.630 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 2,945,618 + -0.6 2,945,618 + +0.1 2,945,657 - -2.1 2,945,657 - +1.2 2,945,681 - +0.2 2,945,785 + -2.7 2,945,785 + -0.5 2,945,786 - -0.0 2,945,787 + -1.3 2,945,787 + -1.4 2,945,787 + -0.2 2,945,787 + -0.2 2,945,805 + -0.8 2,945,850 - -0.7 2,945,861 + -0.4 2,945,879 + +0.0 2,945,879 + -2.3 2,945,880 - -0.5 2,946,089 + purN HEPCGN_20970 0.18 -0.7 2,946,148 - purN HEPCGN_20970 0.27 -0.6 2,946,166 - purN HEPCGN_20970 0.30 +0.0 2,946,171 - purN HEPCGN_20970 0.31 -0.2 2,946,216 - purN HEPCGN_20970 0.38 -0.0 2,946,382 + purN HEPCGN_20970 0.64 +1.4 2,946,383 - purN HEPCGN_20970 0.64 -1.4 2,946,383 - purN HEPCGN_20970 0.64 -3.2 2,946,383 - purN HEPCGN_20970 0.64 +0.2 2,946,415 + purN HEPCGN_20970 0.69 -1.0 2,946,415 + purN HEPCGN_20970 0.69 -1.7 2,946,452 + purN HEPCGN_20970 0.74 -1.9 2,946,453 - purN HEPCGN_20970 0.75 -0.8 2,946,537 - purN HEPCGN_20970 0.88 +0.9 2,946,563 + -1.0 2,946,563 + -0.4 2,946,578 + -0.7 2,946,596 + -0.3 2,946,596 + -0.6 2,946,608 + -0.6 2,946,736 + purM HEPCGN_20975 0.12 -1.2 2,946,737 - purM HEPCGN_20975 0.12 -1.4 2,946,966 + purM HEPCGN_20975 0.34 -3.8 2,946,967 - purM HEPCGN_20975 0.34 -0.7 2,947,191 + purM HEPCGN_20975 0.56 +1.6 2,947,192 - purM HEPCGN_20975 0.56 -0.9 2,947,206 + purM HEPCGN_20975 0.57 -3.1 2,947,206 + purM HEPCGN_20975 0.57 -1.0 2,947,206 + purM HEPCGN_20975 0.57 -1.8 2,947,329 + purM HEPCGN_20975 0.69 -1.3 2,947,330 - purM HEPCGN_20975 0.69 -1.6 2,947,421 + purM HEPCGN_20975 0.78 -0.6 2,947,448 - purM HEPCGN_20975 0.80 -1.1 2,947,566 - -0.7 2,947,566 - -1.7 2,947,594 + +0.5 2,947,626 + -0.7 2,947,630 + +0.4 2,947,631 - -0.1 2,947,631 - -2.3 2,947,672 - +0.4 2,947,707 - +0.2 2,947,786 + -2.1 2,947,793 - +0.1 2,947,928 + -2.2 2,947,928 + -0.5 2,947,930 + -0.6 2,947,931 - +0.9 2,947,975 + +0.3 2,947,976 - -1.6 2,948,042 - upp HEPCGN_20980 0.11 -1.1 2,948,096 + upp HEPCGN_20980 0.19 -0.4 2,948,097 - upp HEPCGN_20980 0.19 -3.4 2,948,179 + upp HEPCGN_20980 0.32 -0.2 2,948,280 - upp HEPCGN_20980 0.48 -0.5 2,948,348 - upp HEPCGN_20980 0.59 +0.5 2,948,510 + upp HEPCGN_20980 0.85 +0.7 2,948,510 + upp HEPCGN_20980 0.85 +0.1 2,948,511 - upp HEPCGN_20980 0.85 -1.0 2,948,511 - upp HEPCGN_20980 0.85 +1.9 2,948,511 - upp HEPCGN_20980 0.85 -1.6 2,948,512 + upp HEPCGN_20980 0.85 +1.2 2,948,550 - -0.1 2,948,554 + -1.3 2,948,554 + -2.1 2,948,554 + -0.4 2,948,617 + +0.6 2,948,618 - +0.7 2,948,630 - +0.4
Or see this region's nucleotide sequence