Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rlmN and rodZ are separated by 284 nucleotides rodZ and ispG are separated by 26 nucleotides
HEPCGN_20830: rlmN - bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN, at 2,909,318 to 2,910,472
rlmN
HEPCGN_20835: rodZ - cytoskeleton protein RodZ, at 2,910,757 to 2,911,767
rodZ
HEPCGN_20840: ispG - flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase, at 2,911,794 to 2,912,912
ispG
Position (kb)
2910
2911
2912 Strain fitness (log2 ratio)
-2
-1
0
1 at 2909.961 kb on + strand, within rlmN at 2909.962 kb on - strand, within rlmN at 2910.071 kb on + strand, within rlmN at 2910.152 kb on + strand, within rlmN at 2910.152 kb on + strand, within rlmN at 2910.153 kb on - strand, within rlmN at 2910.153 kb on - strand, within rlmN at 2910.220 kb on + strand, within rlmN at 2910.273 kb on - strand, within rlmN at 2910.273 kb on - strand, within rlmN at 2910.327 kb on - strand, within rlmN at 2910.340 kb on + strand, within rlmN at 2910.341 kb on - strand, within rlmN at 2910.341 kb on - strand, within rlmN at 2910.421 kb on + strand at 2910.421 kb on + strand at 2910.421 kb on + strand at 2910.431 kb on - strand at 2910.496 kb on + strand at 2910.497 kb on - strand at 2910.497 kb on - strand at 2910.500 kb on + strand at 2910.501 kb on - strand at 2910.533 kb on - strand at 2910.588 kb on + strand at 2910.589 kb on - strand at 2910.589 kb on - strand at 2910.592 kb on + strand at 2910.592 kb on + strand at 2910.593 kb on - strand at 2910.593 kb on - strand at 2910.607 kb on - strand at 2910.611 kb on + strand at 2910.612 kb on - strand at 2910.656 kb on + strand at 2910.656 kb on + strand at 2910.656 kb on + strand at 2910.657 kb on - strand at 2910.657 kb on - strand at 2910.657 kb on - strand at 2910.658 kb on + strand at 2910.658 kb on + strand at 2910.658 kb on + strand at 2910.658 kb on + strand at 2910.659 kb on - strand at 2911.247 kb on + strand, within rodZ at 2911.247 kb on + strand, within rodZ at 2911.252 kb on - strand, within rodZ at 2911.596 kb on + strand, within rodZ at 2911.633 kb on + strand, within rodZ at 2911.634 kb on - strand, within rodZ at 2911.675 kb on + strand at 2911.765 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 2,909,961 + rlmN HEPCGN_20830 0.56 -0.7 2,909,962 - rlmN HEPCGN_20830 0.56 +1.0 2,910,071 + rlmN HEPCGN_20830 0.65 +0.5 2,910,152 + rlmN HEPCGN_20830 0.72 +0.9 2,910,152 + rlmN HEPCGN_20830 0.72 +0.1 2,910,153 - rlmN HEPCGN_20830 0.72 -0.6 2,910,153 - rlmN HEPCGN_20830 0.72 +0.7 2,910,220 + rlmN HEPCGN_20830 0.78 +0.6 2,910,273 - rlmN HEPCGN_20830 0.83 -0.2 2,910,273 - rlmN HEPCGN_20830 0.83 +0.2 2,910,327 - rlmN HEPCGN_20830 0.87 -0.1 2,910,340 + rlmN HEPCGN_20830 0.88 -0.5 2,910,341 - rlmN HEPCGN_20830 0.89 +0.7 2,910,341 - rlmN HEPCGN_20830 0.89 -1.9 2,910,421 + +1.6 2,910,421 + -0.6 2,910,421 + +1.5 2,910,431 - -2.3 2,910,496 + +0.3 2,910,497 - +0.6 2,910,497 - -0.0 2,910,500 + +1.4 2,910,501 - -2.3 2,910,533 - +1.2 2,910,588 + +0.7 2,910,589 - -1.1 2,910,589 - -0.2 2,910,592 + -1.8 2,910,592 + +0.3 2,910,593 - -1.0 2,910,593 - -1.1 2,910,607 - +1.3 2,910,611 + -1.2 2,910,612 - +0.3 2,910,656 + +0.5 2,910,656 + -0.2 2,910,656 + -0.9 2,910,657 - -0.3 2,910,657 - -1.5 2,910,657 - -0.6 2,910,658 + -0.5 2,910,658 + +0.2 2,910,658 + -0.2 2,910,658 + +1.7 2,910,659 - -1.2 2,911,247 + rodZ HEPCGN_20835 0.48 +1.5 2,911,247 + rodZ HEPCGN_20835 0.48 +0.8 2,911,252 - rodZ HEPCGN_20835 0.49 +0.0 2,911,596 + rodZ HEPCGN_20835 0.83 +0.4 2,911,633 + rodZ HEPCGN_20835 0.87 +1.5 2,911,634 - rodZ HEPCGN_20835 0.87 +0.5 2,911,675 + -0.3 2,911,765 + -0.0
Or see this region's nucleotide sequence