Strain Fitness in Escherichia coli ECOR38 around HEPCGN_20755

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrmJ and iscR are separated by 270 nucleotidesiscR and iscS are separated by 111 nucleotides HEPCGN_20750: trmJ - tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ, at 2,890,924 to 2,891,664 trmJ HEPCGN_20755: iscR - Fe-S cluster assembly transcriptional regulator IscR, at 2,891,935 to 2,892,423 iscR HEPCGN_20760: iscS - cysteine desulfurase, at 2,892,535 to 2,893,749 iscS Position (kb) 2891 2892 2893Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2891.276 kb on + strand, within trmJat 2891.311 kb on + strand, within trmJat 2891.554 kb on - strand, within trmJat 2891.628 kb on + strandat 2891.629 kb on - strandat 2891.650 kb on - strandat 2891.652 kb on + strandat 2891.709 kb on + strandat 2891.709 kb on + strandat 2891.710 kb on - strandat 2891.929 kb on + strandat 2891.949 kb on + strandat 2892.055 kb on + strand, within iscRat 2892.194 kb on + strand, within iscRat 2892.306 kb on + strand, within iscRat 2892.306 kb on + strand, within iscRat 2892.324 kb on + strand, within iscRat 2892.465 kb on + strandat 2892.517 kb on + strandat 2892.517 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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2,891,276 + trmJ HEPCGN_20750 0.48 -0.4
2,891,311 + trmJ HEPCGN_20750 0.52 +1.5
2,891,554 - trmJ HEPCGN_20750 0.85 +0.2
2,891,628 + -1.2
2,891,629 - +0.0
2,891,650 - -0.0
2,891,652 + +0.4
2,891,709 + +0.5
2,891,709 + +0.1
2,891,710 - +0.7
2,891,929 + -1.6
2,891,949 + -0.2
2,892,055 + iscR HEPCGN_20755 0.25 +0.2
2,892,194 + iscR HEPCGN_20755 0.53 +0.5
2,892,306 + iscR HEPCGN_20755 0.76 +0.7
2,892,306 + iscR HEPCGN_20755 0.76 +4.2
2,892,324 + iscR HEPCGN_20755 0.80 -0.1
2,892,465 + -0.2
2,892,517 + +0.1
2,892,517 + -2.8

Or see this region's nucleotide sequence