Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpoE and rseA are separated by 32 nucleotides rseA and rseB overlap by 1 nucleotides rseB and rseC overlap by 4 nucleotides
HEPCGN_20520: rpoE - RNA polymerase sigma factor RpoE, at 2,844,331 to 2,844,906
rpoE
HEPCGN_20525: rseA - anti-sigma-E factor RseA, at 2,844,939 to 2,845,607
rseA
HEPCGN_20530: rseB - sigma-E factor regulatory protein RseB, at 2,845,607 to 2,846,563
rseB
HEPCGN_20540: rseC - SoxR-reducing system protein RseC, at 2,846,560 to 2,847,039
rseC
Position (kb)
2844
2845
2846 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2843.962 kb on + strand at 2844.017 kb on - strand at 2844.055 kb on - strand at 2844.073 kb on - strand at 2844.073 kb on - strand at 2844.085 kb on - strand at 2844.116 kb on + strand at 2844.116 kb on + strand at 2844.116 kb on + strand at 2844.117 kb on - strand at 2844.131 kb on - strand at 2844.139 kb on - strand at 2844.139 kb on - strand at 2844.165 kb on + strand at 2844.165 kb on + strand at 2844.166 kb on - strand at 2844.166 kb on - strand at 2844.166 kb on - strand at 2844.166 kb on - strand at 2844.167 kb on + strand at 2844.173 kb on + strand at 2844.173 kb on + strand at 2844.173 kb on + strand at 2844.174 kb on - strand at 2844.174 kb on - strand at 2844.177 kb on + strand at 2844.273 kb on + strand at 2844.541 kb on + strand, within rpoE at 2844.544 kb on - strand, within rpoE at 2844.571 kb on - strand, within rpoE at 2844.692 kb on + strand, within rpoE at 2844.870 kb on + strand at 2844.932 kb on - strand at 2844.935 kb on - strand at 2844.935 kb on - strand at 2844.998 kb on + strand at 2845.013 kb on + strand, within rseA at 2845.051 kb on + strand, within rseA at 2845.077 kb on - strand, within rseA at 2845.142 kb on - strand, within rseA at 2845.299 kb on - strand, within rseA at 2845.319 kb on + strand, within rseA at 2845.365 kb on - strand, within rseA at 2845.367 kb on + strand, within rseA at 2845.368 kb on - strand, within rseA at 2845.453 kb on + strand, within rseA at 2845.564 kb on + strand at 2845.653 kb on + strand at 2845.698 kb on + strand at 2845.881 kb on - strand, within rseB at 2845.952 kb on + strand, within rseB at 2845.952 kb on + strand, within rseB at 2845.952 kb on + strand, within rseB at 2845.953 kb on - strand, within rseB at 2845.980 kb on - strand, within rseB at 2845.980 kb on - strand, within rseB at 2846.011 kb on + strand, within rseB at 2846.011 kb on + strand, within rseB at 2846.036 kb on - strand, within rseB at 2846.059 kb on + strand, within rseB at 2846.066 kb on + strand, within rseB at 2846.072 kb on + strand, within rseB at 2846.072 kb on + strand, within rseB at 2846.073 kb on - strand, within rseB at 2846.073 kb on - strand, within rseB at 2846.073 kb on - strand, within rseB at 2846.073 kb on - strand, within rseB at 2846.170 kb on + strand, within rseB at 2846.170 kb on + strand, within rseB at 2846.198 kb on - strand, within rseB at 2846.248 kb on - strand, within rseB at 2846.470 kb on - strand at 2846.540 kb on - strand at 2846.542 kb on + strand at 2846.543 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 2,843,962 + +0.1 2,844,017 - -2.0 2,844,055 - -0.2 2,844,073 - -1.5 2,844,073 - -1.7 2,844,085 - +0.2 2,844,116 + +0.6 2,844,116 + -0.4 2,844,116 + -0.7 2,844,117 - -0.2 2,844,131 - +1.8 2,844,139 - +0.6 2,844,139 - +1.0 2,844,165 + -0.7 2,844,165 + +0.3 2,844,166 - +1.4 2,844,166 - -1.9 2,844,166 - -0.3 2,844,166 - -1.6 2,844,167 + -0.8 2,844,173 + -1.4 2,844,173 + -0.3 2,844,173 + -1.2 2,844,174 - +0.2 2,844,174 - -1.3 2,844,177 + +0.6 2,844,273 + +0.5 2,844,541 + rpoE HEPCGN_20520 0.36 +1.4 2,844,544 - rpoE HEPCGN_20520 0.37 +1.8 2,844,571 - rpoE HEPCGN_20520 0.42 +0.6 2,844,692 + rpoE HEPCGN_20520 0.63 +0.1 2,844,870 + +0.6 2,844,932 - -1.2 2,844,935 - +2.6 2,844,935 - +0.2 2,844,998 + -1.1 2,845,013 + rseA HEPCGN_20525 0.11 +0.1 2,845,051 + rseA HEPCGN_20525 0.17 +0.8 2,845,077 - rseA HEPCGN_20525 0.21 +1.1 2,845,142 - rseA HEPCGN_20525 0.30 +2.0 2,845,299 - rseA HEPCGN_20525 0.54 -0.8 2,845,319 + rseA HEPCGN_20525 0.57 +1.3 2,845,365 - rseA HEPCGN_20525 0.64 +1.5 2,845,367 + rseA HEPCGN_20525 0.64 -1.3 2,845,368 - rseA HEPCGN_20525 0.64 +2.2 2,845,453 + rseA HEPCGN_20525 0.77 +2.2 2,845,564 + +1.2 2,845,653 + -0.5 2,845,698 + -0.2 2,845,881 - rseB HEPCGN_20530 0.29 -0.1 2,845,952 + rseB HEPCGN_20530 0.36 +2.8 2,845,952 + rseB HEPCGN_20530 0.36 +3.4 2,845,952 + rseB HEPCGN_20530 0.36 -0.6 2,845,953 - rseB HEPCGN_20530 0.36 -0.0 2,845,980 - rseB HEPCGN_20530 0.39 +0.5 2,845,980 - rseB HEPCGN_20530 0.39 -1.3 2,846,011 + rseB HEPCGN_20530 0.42 +0.4 2,846,011 + rseB HEPCGN_20530 0.42 -0.9 2,846,036 - rseB HEPCGN_20530 0.45 +1.0 2,846,059 + rseB HEPCGN_20530 0.47 -0.2 2,846,066 + rseB HEPCGN_20530 0.48 +0.2 2,846,072 + rseB HEPCGN_20530 0.49 +1.5 2,846,072 + rseB HEPCGN_20530 0.49 +0.3 2,846,073 - rseB HEPCGN_20530 0.49 -0.1 2,846,073 - rseB HEPCGN_20530 0.49 +1.6 2,846,073 - rseB HEPCGN_20530 0.49 +0.6 2,846,073 - rseB HEPCGN_20530 0.49 -0.3 2,846,170 + rseB HEPCGN_20530 0.59 +0.5 2,846,170 + rseB HEPCGN_20530 0.59 -0.4 2,846,198 - rseB HEPCGN_20530 0.62 +1.2 2,846,248 - rseB HEPCGN_20530 0.67 -0.5 2,846,470 - +0.6 2,846,540 - +3.0 2,846,542 + +1.6 2,846,543 - -0.3
Or see this region's nucleotide sequence