Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt truD and surE overlap by 20 nucleotides surE and pcm overlap by 7 nucleotides pcm and nlpD are separated by 139 nucleotides
HEPCGN_19655: truD - tRNA pseudouridine(13) synthase TruD, at 2,687,545 to 2,688,594
truD
HEPCGN_19660: surE - 5'/3'-nucleotidase SurE, at 2,688,575 to 2,689,336
surE
HEPCGN_19665: pcm - protein-L-isoaspartate O-methyltransferase, at 2,689,330 to 2,689,956
pcm
HEPCGN_19670: nlpD - murein hydrolase activator NlpD, at 2,690,096 to 2,691,235
nlpD
Position (kb)
2689
2690 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2688.399 kb on - strand, within truD at 2688.399 kb on - strand, within truD at 2688.467 kb on + strand, within truD at 2688.555 kb on + strand at 2688.555 kb on + strand at 2688.555 kb on + strand at 2688.555 kb on + strand at 2688.555 kb on + strand at 2688.556 kb on - strand at 2688.556 kb on - strand at 2688.556 kb on - strand at 2688.556 kb on - strand at 2688.575 kb on - strand at 2688.607 kb on - strand at 2688.607 kb on - strand at 2688.622 kb on - strand at 2688.622 kb on - strand at 2688.736 kb on + strand, within surE at 2688.737 kb on - strand, within surE at 2688.754 kb on + strand, within surE at 2688.878 kb on - strand, within surE at 2688.958 kb on + strand, within surE at 2688.959 kb on - strand, within surE at 2688.965 kb on + strand, within surE at 2689.043 kb on - strand, within surE at 2689.043 kb on - strand, within surE at 2689.084 kb on + strand, within surE at 2689.084 kb on + strand, within surE at 2689.085 kb on - strand, within surE at 2689.105 kb on + strand, within surE at 2689.106 kb on - strand, within surE at 2689.106 kb on - strand, within surE at 2689.156 kb on + strand, within surE at 2689.156 kb on + strand, within surE at 2689.224 kb on + strand, within surE at 2689.225 kb on - strand, within surE at 2689.235 kb on + strand, within surE at 2689.236 kb on - strand, within surE at 2689.236 kb on - strand, within surE at 2689.236 kb on - strand, within surE at 2689.266 kb on + strand at 2689.286 kb on + strand at 2689.286 kb on + strand at 2689.287 kb on - strand at 2689.287 kb on - strand at 2689.287 kb on - strand at 2689.287 kb on - strand at 2689.346 kb on + strand at 2689.346 kb on + strand at 2689.380 kb on + strand at 2689.381 kb on - strand at 2689.484 kb on + strand, within pcm at 2689.514 kb on - strand, within pcm at 2689.515 kb on + strand, within pcm at 2689.515 kb on + strand, within pcm at 2689.698 kb on + strand, within pcm at 2689.861 kb on + strand, within pcm at 2689.969 kb on + strand at 2689.970 kb on - strand at 2689.988 kb on + strand at 2690.037 kb on - strand at 2690.058 kb on - strand at 2690.058 kb on - strand at 2690.058 kb on - strand at 2690.093 kb on + strand at 2690.094 kb on - strand at 2690.285 kb on - strand, within nlpD at 2690.386 kb on - strand, within nlpD at 2690.458 kb on + strand, within nlpD at 2690.462 kb on + strand, within nlpD at 2690.462 kb on + strand, within nlpD at 2690.464 kb on + strand, within nlpD at 2690.464 kb on + strand, within nlpD at 2690.464 kb on + strand, within nlpD at 2690.465 kb on - strand, within nlpD at 2690.494 kb on + strand, within nlpD at 2690.495 kb on - strand, within nlpD at 2690.506 kb on + strand, within nlpD at 2690.545 kb on + strand, within nlpD at 2690.558 kb on + strand, within nlpD at 2690.584 kb on - strand, within nlpD at 2690.716 kb on + strand, within nlpD at 2690.716 kb on + strand, within nlpD at 2690.716 kb on + strand, within nlpD at 2690.717 kb on - strand, within nlpD at 2690.721 kb on - strand, within nlpD at 2690.803 kb on - strand, within nlpD at 2690.845 kb on + strand, within nlpD at 2690.845 kb on + strand, within nlpD at 2690.845 kb on + strand, within nlpD at 2690.846 kb on - strand, within nlpD at 2690.846 kb on - strand, within nlpD at 2690.864 kb on - strand, within nlpD
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 2,688,399 - truD HEPCGN_19655 0.81 -1.1 2,688,399 - truD HEPCGN_19655 0.81 -1.4 2,688,467 + truD HEPCGN_19655 0.88 +3.7 2,688,555 + +0.8 2,688,555 + +1.7 2,688,555 + -1.3 2,688,555 + -0.7 2,688,555 + -0.9 2,688,556 - -1.3 2,688,556 - -0.4 2,688,556 - +0.3 2,688,556 - +0.4 2,688,575 - -0.3 2,688,607 - -0.7 2,688,607 - -0.5 2,688,622 - +0.5 2,688,622 - +1.1 2,688,736 + surE HEPCGN_19660 0.21 +0.1 2,688,737 - surE HEPCGN_19660 0.21 -0.4 2,688,754 + surE HEPCGN_19660 0.23 -0.4 2,688,878 - surE HEPCGN_19660 0.40 +0.9 2,688,958 + surE HEPCGN_19660 0.50 -1.1 2,688,959 - surE HEPCGN_19660 0.50 +0.2 2,688,965 + surE HEPCGN_19660 0.51 +1.0 2,689,043 - surE HEPCGN_19660 0.61 -0.3 2,689,043 - surE HEPCGN_19660 0.61 -1.8 2,689,084 + surE HEPCGN_19660 0.67 -0.3 2,689,084 + surE HEPCGN_19660 0.67 +0.7 2,689,085 - surE HEPCGN_19660 0.67 -0.9 2,689,105 + surE HEPCGN_19660 0.70 -0.4 2,689,106 - surE HEPCGN_19660 0.70 -2.1 2,689,106 - surE HEPCGN_19660 0.70 -0.4 2,689,156 + surE HEPCGN_19660 0.76 -0.7 2,689,156 + surE HEPCGN_19660 0.76 -0.6 2,689,224 + surE HEPCGN_19660 0.85 -2.0 2,689,225 - surE HEPCGN_19660 0.85 -0.1 2,689,235 + surE HEPCGN_19660 0.87 +0.2 2,689,236 - surE HEPCGN_19660 0.87 -0.3 2,689,236 - surE HEPCGN_19660 0.87 +0.0 2,689,236 - surE HEPCGN_19660 0.87 -1.8 2,689,266 + -0.4 2,689,286 + +0.7 2,689,286 + -1.3 2,689,287 - -0.2 2,689,287 - -1.9 2,689,287 - +0.8 2,689,287 - -0.5 2,689,346 + -0.3 2,689,346 + +0.6 2,689,380 + -0.1 2,689,381 - -0.1 2,689,484 + pcm HEPCGN_19665 0.25 -0.9 2,689,514 - pcm HEPCGN_19665 0.29 -0.2 2,689,515 + pcm HEPCGN_19665 0.30 -1.9 2,689,515 + pcm HEPCGN_19665 0.30 -0.1 2,689,698 + pcm HEPCGN_19665 0.59 -1.6 2,689,861 + pcm HEPCGN_19665 0.85 -0.5 2,689,969 + -0.3 2,689,970 - +1.3 2,689,988 + -0.9 2,690,037 - +0.8 2,690,058 - -0.7 2,690,058 - -0.0 2,690,058 - +1.3 2,690,093 + -0.7 2,690,094 - -0.1 2,690,285 - nlpD HEPCGN_19670 0.17 +0.5 2,690,386 - nlpD HEPCGN_19670 0.25 +1.0 2,690,458 + nlpD HEPCGN_19670 0.32 -2.1 2,690,462 + nlpD HEPCGN_19670 0.32 -0.1 2,690,462 + nlpD HEPCGN_19670 0.32 +0.0 2,690,464 + nlpD HEPCGN_19670 0.32 +1.0 2,690,464 + nlpD HEPCGN_19670 0.32 +2.4 2,690,464 + nlpD HEPCGN_19670 0.32 -1.0 2,690,465 - nlpD HEPCGN_19670 0.32 -1.0 2,690,494 + nlpD HEPCGN_19670 0.35 +2.8 2,690,495 - nlpD HEPCGN_19670 0.35 -0.1 2,690,506 + nlpD HEPCGN_19670 0.36 -0.8 2,690,545 + nlpD HEPCGN_19670 0.39 +0.9 2,690,558 + nlpD HEPCGN_19670 0.41 -1.7 2,690,584 - nlpD HEPCGN_19670 0.43 +0.0 2,690,716 + nlpD HEPCGN_19670 0.54 +0.4 2,690,716 + nlpD HEPCGN_19670 0.54 -0.2 2,690,716 + nlpD HEPCGN_19670 0.54 -2.1 2,690,717 - nlpD HEPCGN_19670 0.54 +0.1 2,690,721 - nlpD HEPCGN_19670 0.55 +0.1 2,690,803 - nlpD HEPCGN_19670 0.62 -0.5 2,690,845 + nlpD HEPCGN_19670 0.66 +0.4 2,690,845 + nlpD HEPCGN_19670 0.66 -0.4 2,690,845 + nlpD HEPCGN_19670 0.66 +2.5 2,690,846 - nlpD HEPCGN_19670 0.66 +1.1 2,690,846 - nlpD HEPCGN_19670 0.66 +0.9 2,690,864 - nlpD HEPCGN_19670 0.67 -1.2
Or see this region's nucleotide sequence