Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt csdA and ygdI are separated by 191 nucleotides ygdI and gcvA are separated by 350 nucleotides gcvA and ygdD are separated by 18 nucleotides ygdD and rlmM overlap by 8 nucleotides
HEPCGN_19360: csdA - cysteine desulfurase CsdA, at 2,625,873 to 2,627,078
csdA
HEPCGN_19365: ygdI - Uncharacterized lipoprotein YgdI, at 2,627,270 to 2,627,497
ygdI
HEPCGN_19375: gcvA - glycine cleavage system transcriptional regulator GcvA, at 2,627,848 to 2,628,765
gcvA
HEPCGN_19380: ygdD - DUF423 domain-containing protein, at 2,628,784 to 2,629,179
ygdD
HEPCGN_19385: rlmM - 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM, at 2,629,172 to 2,630,272
rlmM
Position (kb)
2627
2628
2629 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2626.854 kb on + strand, within csdA at 2626.854 kb on + strand, within csdA at 2626.988 kb on + strand at 2626.989 kb on - strand at 2627.081 kb on + strand at 2627.127 kb on + strand at 2627.128 kb on - strand at 2627.141 kb on + strand at 2627.142 kb on - strand at 2627.155 kb on - strand at 2627.180 kb on + strand at 2627.190 kb on - strand at 2627.198 kb on + strand at 2627.204 kb on + strand at 2627.236 kb on - strand at 2627.236 kb on - strand at 2627.290 kb on + strand at 2627.342 kb on + strand, within ygdI at 2627.342 kb on + strand, within ygdI at 2627.461 kb on + strand, within ygdI at 2627.542 kb on + strand at 2627.543 kb on - strand at 2627.543 kb on - strand at 2627.547 kb on + strand at 2627.570 kb on - strand at 2627.570 kb on - strand at 2627.576 kb on - strand at 2627.729 kb on - strand at 2627.730 kb on + strand at 2627.731 kb on - strand at 2627.731 kb on - strand at 2627.731 kb on - strand at 2627.731 kb on - strand at 2627.734 kb on + strand at 2627.841 kb on - strand at 2627.958 kb on - strand, within gcvA at 2627.958 kb on - strand, within gcvA at 2627.958 kb on - strand, within gcvA at 2627.958 kb on - strand, within gcvA at 2628.146 kb on + strand, within gcvA at 2628.252 kb on + strand, within gcvA at 2628.252 kb on + strand, within gcvA at 2628.252 kb on + strand, within gcvA at 2628.253 kb on - strand, within gcvA at 2628.312 kb on - strand, within gcvA at 2628.342 kb on - strand, within gcvA at 2628.414 kb on + strand, within gcvA at 2628.414 kb on + strand, within gcvA at 2628.415 kb on - strand, within gcvA at 2628.419 kb on + strand, within gcvA at 2628.419 kb on + strand, within gcvA at 2628.422 kb on + strand, within gcvA at 2628.423 kb on - strand, within gcvA at 2628.423 kb on - strand, within gcvA at 2628.423 kb on - strand, within gcvA at 2628.454 kb on + strand, within gcvA at 2628.454 kb on + strand, within gcvA at 2628.454 kb on + strand, within gcvA at 2628.457 kb on - strand, within gcvA at 2628.457 kb on - strand, within gcvA at 2628.508 kb on - strand, within gcvA at 2628.618 kb on + strand, within gcvA at 2628.637 kb on - strand, within gcvA at 2628.754 kb on + strand at 2628.867 kb on - strand, within ygdD at 2628.947 kb on + strand, within ygdD at 2628.948 kb on - strand, within ygdD at 2628.981 kb on + strand, within ygdD at 2629.178 kb on - strand at 2629.185 kb on + strand at 2629.185 kb on + strand at 2629.185 kb on + strand at 2629.186 kb on - strand at 2629.302 kb on - strand, within rlmM at 2629.302 kb on - strand, within rlmM at 2629.310 kb on + strand, within rlmM at 2629.331 kb on - strand, within rlmM at 2629.336 kb on - strand, within rlmM at 2629.430 kb on - strand, within rlmM at 2629.430 kb on - strand, within rlmM at 2629.458 kb on + strand, within rlmM at 2629.589 kb on + strand, within rlmM at 2629.589 kb on + strand, within rlmM at 2629.590 kb on - strand, within rlmM at 2629.620 kb on + strand, within rlmM at 2629.620 kb on + strand, within rlmM at 2629.620 kb on + strand, within rlmM at 2629.620 kb on + strand, within rlmM at 2629.621 kb on - strand, within rlmM at 2629.621 kb on - strand, within rlmM at 2629.627 kb on + strand, within rlmM at 2629.646 kb on + strand, within rlmM at 2629.647 kb on - strand, within rlmM at 2629.661 kb on + strand, within rlmM at 2629.673 kb on - strand, within rlmM at 2629.685 kb on + strand, within rlmM at 2629.685 kb on + strand, within rlmM at 2629.686 kb on - strand, within rlmM at 2629.686 kb on - strand, within rlmM
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 2,626,854 + csdA HEPCGN_19360 0.81 +2.2 2,626,854 + csdA HEPCGN_19360 0.81 +1.1 2,626,988 + +0.9 2,626,989 - -0.4 2,627,081 + +0.3 2,627,127 + +0.7 2,627,128 - -0.5 2,627,141 + +0.0 2,627,142 - -2.5 2,627,155 - -0.4 2,627,180 + -0.2 2,627,190 - -0.1 2,627,198 + +0.6 2,627,204 + +0.6 2,627,236 - -0.6 2,627,236 - +0.0 2,627,290 + +2.4 2,627,342 + ygdI HEPCGN_19365 0.32 -0.2 2,627,342 + ygdI HEPCGN_19365 0.32 -0.5 2,627,461 + ygdI HEPCGN_19365 0.84 -0.1 2,627,542 + +1.8 2,627,543 - -0.6 2,627,543 - -0.2 2,627,547 + -1.2 2,627,570 - +0.6 2,627,570 - +0.1 2,627,576 - +0.5 2,627,729 - +0.8 2,627,730 + +0.6 2,627,731 - +1.1 2,627,731 - +0.8 2,627,731 - -1.2 2,627,731 - +2.2 2,627,734 + +0.9 2,627,841 - +0.2 2,627,958 - gcvA HEPCGN_19375 0.12 +1.2 2,627,958 - gcvA HEPCGN_19375 0.12 +1.1 2,627,958 - gcvA HEPCGN_19375 0.12 +0.8 2,627,958 - gcvA HEPCGN_19375 0.12 -2.0 2,628,146 + gcvA HEPCGN_19375 0.32 +0.6 2,628,252 + gcvA HEPCGN_19375 0.44 +1.2 2,628,252 + gcvA HEPCGN_19375 0.44 +1.7 2,628,252 + gcvA HEPCGN_19375 0.44 +2.7 2,628,253 - gcvA HEPCGN_19375 0.44 +1.9 2,628,312 - gcvA HEPCGN_19375 0.51 +3.0 2,628,342 - gcvA HEPCGN_19375 0.54 +2.5 2,628,414 + gcvA HEPCGN_19375 0.62 +0.6 2,628,414 + gcvA HEPCGN_19375 0.62 +1.6 2,628,415 - gcvA HEPCGN_19375 0.62 +0.6 2,628,419 + gcvA HEPCGN_19375 0.62 +1.1 2,628,419 + gcvA HEPCGN_19375 0.62 +0.8 2,628,422 + gcvA HEPCGN_19375 0.63 +1.4 2,628,423 - gcvA HEPCGN_19375 0.63 +1.9 2,628,423 - gcvA HEPCGN_19375 0.63 +0.5 2,628,423 - gcvA HEPCGN_19375 0.63 +1.0 2,628,454 + gcvA HEPCGN_19375 0.66 +3.5 2,628,454 + gcvA HEPCGN_19375 0.66 +1.0 2,628,454 + gcvA HEPCGN_19375 0.66 +1.5 2,628,457 - gcvA HEPCGN_19375 0.66 +0.0 2,628,457 - gcvA HEPCGN_19375 0.66 -1.1 2,628,508 - gcvA HEPCGN_19375 0.72 +1.5 2,628,618 + gcvA HEPCGN_19375 0.84 +1.9 2,628,637 - gcvA HEPCGN_19375 0.86 +1.6 2,628,754 + +0.4 2,628,867 - ygdD HEPCGN_19380 0.21 -0.2 2,628,947 + ygdD HEPCGN_19380 0.41 +1.2 2,628,948 - ygdD HEPCGN_19380 0.41 -0.0 2,628,981 + ygdD HEPCGN_19380 0.50 -0.1 2,629,178 - +0.1 2,629,185 + -0.4 2,629,185 + -1.2 2,629,185 + +0.8 2,629,186 - -1.5 2,629,302 - rlmM HEPCGN_19385 0.12 -0.8 2,629,302 - rlmM HEPCGN_19385 0.12 +1.9 2,629,310 + rlmM HEPCGN_19385 0.13 -0.5 2,629,331 - rlmM HEPCGN_19385 0.14 -1.3 2,629,336 - rlmM HEPCGN_19385 0.15 -0.7 2,629,430 - rlmM HEPCGN_19385 0.23 +0.2 2,629,430 - rlmM HEPCGN_19385 0.23 -1.7 2,629,458 + rlmM HEPCGN_19385 0.26 -0.8 2,629,589 + rlmM HEPCGN_19385 0.38 -0.1 2,629,589 + rlmM HEPCGN_19385 0.38 +1.7 2,629,590 - rlmM HEPCGN_19385 0.38 -0.3 2,629,620 + rlmM HEPCGN_19385 0.41 -0.5 2,629,620 + rlmM HEPCGN_19385 0.41 -0.4 2,629,620 + rlmM HEPCGN_19385 0.41 +0.7 2,629,620 + rlmM HEPCGN_19385 0.41 -0.2 2,629,621 - rlmM HEPCGN_19385 0.41 -0.3 2,629,621 - rlmM HEPCGN_19385 0.41 -0.3 2,629,627 + rlmM HEPCGN_19385 0.41 -1.1 2,629,646 + rlmM HEPCGN_19385 0.43 +1.2 2,629,647 - rlmM HEPCGN_19385 0.43 +0.2 2,629,661 + rlmM HEPCGN_19385 0.44 -1.4 2,629,673 - rlmM HEPCGN_19385 0.46 -0.3 2,629,685 + rlmM HEPCGN_19385 0.47 -2.0 2,629,685 + rlmM HEPCGN_19385 0.47 +1.5 2,629,686 - rlmM HEPCGN_19385 0.47 -2.0 2,629,686 - rlmM HEPCGN_19385 0.47 -0.1
Or see this region's nucleotide sequence