Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pulJ and ygdB overlap by 4 nucleotides ygdB and ppdC overlap by 16 nucleotides ppdC and recC are separated by 12 nucleotides recC and ptrA are separated by 175 nucleotides
HEPCGN_19280: pulJ - prepilin peptidase-dependent protein, at 2,606,697 to 2,607,260
pulJ
HEPCGN_19285: ygdB - DUF2509 domain-containing protein, at 2,607,257 to 2,607,664
ygdB
HEPCGN_19290: ppdC - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 2,607,649 to 2,607,972
ppdC
HEPCGN_19295: recC - exodeoxyribonuclease V subunit gamma, at 2,607,985 to 2,611,353
recC
HEPCGN_19300: ptrA - pitrilysin, at 2,611,529 to 2,614,417
ptrA
Position (kb)
2607
2608
2609
2610
2611
2612 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2607.185 kb on + strand, within pulJ at 2607.186 kb on - strand, within pulJ at 2607.186 kb on - strand, within pulJ at 2607.298 kb on + strand, within ygdB at 2607.325 kb on - strand, within ygdB at 2607.476 kb on + strand, within ygdB at 2607.510 kb on + strand, within ygdB at 2607.510 kb on + strand, within ygdB at 2607.532 kb on - strand, within ygdB at 2607.758 kb on + strand, within ppdC at 2607.912 kb on - strand, within ppdC at 2607.912 kb on - strand, within ppdC at 2607.970 kb on + strand at 2607.984 kb on + strand at 2607.984 kb on + strand at 2607.984 kb on + strand at 2608.101 kb on + strand at 2608.188 kb on + strand at 2608.397 kb on - strand, within recC at 2608.405 kb on + strand, within recC at 2608.509 kb on + strand, within recC at 2608.552 kb on + strand, within recC at 2608.620 kb on + strand, within recC at 2608.620 kb on + strand, within recC at 2608.621 kb on - strand, within recC at 2608.683 kb on + strand, within recC at 2608.737 kb on + strand, within recC at 2608.806 kb on - strand, within recC at 2608.993 kb on - strand, within recC at 2609.237 kb on - strand, within recC at 2609.288 kb on + strand, within recC at 2609.289 kb on - strand, within recC at 2609.289 kb on - strand, within recC at 2609.293 kb on + strand, within recC at 2609.358 kb on - strand, within recC at 2609.459 kb on - strand, within recC at 2609.502 kb on - strand, within recC at 2609.627 kb on + strand, within recC at 2609.627 kb on + strand, within recC at 2609.628 kb on - strand, within recC at 2609.628 kb on - strand, within recC at 2609.632 kb on + strand, within recC at 2609.764 kb on - strand, within recC at 2609.932 kb on - strand, within recC at 2610.038 kb on - strand, within recC at 2610.166 kb on + strand, within recC at 2610.177 kb on + strand, within recC at 2610.325 kb on - strand, within recC at 2610.427 kb on - strand, within recC at 2610.427 kb on - strand, within recC at 2610.610 kb on - strand, within recC at 2610.636 kb on - strand, within recC at 2610.709 kb on + strand, within recC at 2610.710 kb on - strand, within recC at 2610.839 kb on + strand, within recC at 2610.839 kb on + strand, within recC at 2610.840 kb on - strand, within recC at 2610.900 kb on - strand, within recC at 2610.943 kb on + strand, within recC at 2610.961 kb on - strand, within recC at 2611.039 kb on + strand at 2611.421 kb on + strand at 2611.422 kb on - strand at 2611.422 kb on - strand at 2611.423 kb on + strand at 2611.423 kb on + strand at 2611.423 kb on + strand at 2611.423 kb on + strand at 2611.423 kb on + strand at 2611.423 kb on + strand at 2611.424 kb on - strand at 2611.424 kb on - strand at 2611.424 kb on - strand at 2611.424 kb on - strand at 2611.424 kb on - strand at 2611.424 kb on - strand at 2611.425 kb on + strand at 2611.425 kb on + strand at 2611.431 kb on + strand at 2611.431 kb on + strand at 2611.432 kb on - strand at 2611.557 kb on + strand at 2611.557 kb on + strand at 2611.558 kb on - strand at 2611.589 kb on - strand at 2611.667 kb on - strand at 2611.667 kb on - strand at 2611.684 kb on + strand at 2611.685 kb on - strand at 2611.793 kb on + strand at 2611.856 kb on + strand, within ptrA at 2611.902 kb on - strand, within ptrA at 2612.017 kb on + strand, within ptrA at 2612.018 kb on - strand, within ptrA at 2612.018 kb on - strand, within ptrA at 2612.018 kb on - strand, within ptrA at 2612.132 kb on - strand, within ptrA at 2612.146 kb on - strand, within ptrA at 2612.161 kb on + strand, within ptrA at 2612.202 kb on - strand, within ptrA at 2612.205 kb on - strand, within ptrA at 2612.243 kb on - strand, within ptrA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 2,607,185 + pulJ HEPCGN_19280 0.87 -2.5 2,607,186 - pulJ HEPCGN_19280 0.87 +0.2 2,607,186 - pulJ HEPCGN_19280 0.87 -0.1 2,607,298 + ygdB HEPCGN_19285 0.10 -1.1 2,607,325 - ygdB HEPCGN_19285 0.17 +0.3 2,607,476 + ygdB HEPCGN_19285 0.54 +0.4 2,607,510 + ygdB HEPCGN_19285 0.62 -0.6 2,607,510 + ygdB HEPCGN_19285 0.62 +0.7 2,607,532 - ygdB HEPCGN_19285 0.67 +0.9 2,607,758 + ppdC HEPCGN_19290 0.34 -2.5 2,607,912 - ppdC HEPCGN_19290 0.81 -0.8 2,607,912 - ppdC HEPCGN_19290 0.81 +1.2 2,607,970 + +1.5 2,607,984 + -0.4 2,607,984 + -0.1 2,607,984 + -1.0 2,608,101 + +0.7 2,608,188 + +0.7 2,608,397 - recC HEPCGN_19295 0.12 -1.0 2,608,405 + recC HEPCGN_19295 0.12 +1.0 2,608,509 + recC HEPCGN_19295 0.16 +2.5 2,608,552 + recC HEPCGN_19295 0.17 -0.5 2,608,620 + recC HEPCGN_19295 0.19 -0.9 2,608,620 + recC HEPCGN_19295 0.19 +3.0 2,608,621 - recC HEPCGN_19295 0.19 -1.1 2,608,683 + recC HEPCGN_19295 0.21 -2.6 2,608,737 + recC HEPCGN_19295 0.22 +0.8 2,608,806 - recC HEPCGN_19295 0.24 -1.2 2,608,993 - recC HEPCGN_19295 0.30 -0.0 2,609,237 - recC HEPCGN_19295 0.37 +1.0 2,609,288 + recC HEPCGN_19295 0.39 +0.0 2,609,289 - recC HEPCGN_19295 0.39 +0.2 2,609,289 - recC HEPCGN_19295 0.39 +2.1 2,609,293 + recC HEPCGN_19295 0.39 +2.0 2,609,358 - recC HEPCGN_19295 0.41 +0.2 2,609,459 - recC HEPCGN_19295 0.44 +0.5 2,609,502 - recC HEPCGN_19295 0.45 +0.6 2,609,627 + recC HEPCGN_19295 0.49 +0.1 2,609,627 + recC HEPCGN_19295 0.49 -0.5 2,609,628 - recC HEPCGN_19295 0.49 +0.2 2,609,628 - recC HEPCGN_19295 0.49 +0.1 2,609,632 + recC HEPCGN_19295 0.49 +0.7 2,609,764 - recC HEPCGN_19295 0.53 +0.8 2,609,932 - recC HEPCGN_19295 0.58 -1.7 2,610,038 - recC HEPCGN_19295 0.61 +0.3 2,610,166 + recC HEPCGN_19295 0.65 +1.2 2,610,177 + recC HEPCGN_19295 0.65 +0.9 2,610,325 - recC HEPCGN_19295 0.69 +1.5 2,610,427 - recC HEPCGN_19295 0.72 -1.4 2,610,427 - recC HEPCGN_19295 0.72 -0.1 2,610,610 - recC HEPCGN_19295 0.78 -1.4 2,610,636 - recC HEPCGN_19295 0.79 -1.3 2,610,709 + recC HEPCGN_19295 0.81 +1.2 2,610,710 - recC HEPCGN_19295 0.81 +0.2 2,610,839 + recC HEPCGN_19295 0.85 -0.9 2,610,839 + recC HEPCGN_19295 0.85 -0.7 2,610,840 - recC HEPCGN_19295 0.85 -0.7 2,610,900 - recC HEPCGN_19295 0.87 -0.5 2,610,943 + recC HEPCGN_19295 0.88 +0.4 2,610,961 - recC HEPCGN_19295 0.88 +0.1 2,611,039 + +0.8 2,611,421 + -0.4 2,611,422 - -2.2 2,611,422 - -0.5 2,611,423 + +0.3 2,611,423 + +0.1 2,611,423 + -0.1 2,611,423 + +0.2 2,611,423 + -0.5 2,611,423 + -0.3 2,611,424 - -1.1 2,611,424 - +1.3 2,611,424 - +0.0 2,611,424 - +0.2 2,611,424 - +0.0 2,611,424 - +1.0 2,611,425 + -1.2 2,611,425 + +0.0 2,611,431 + +0.5 2,611,431 + -0.7 2,611,432 - -1.1 2,611,557 + -0.1 2,611,557 + -1.0 2,611,558 - -0.2 2,611,589 - +0.1 2,611,667 - -3.2 2,611,667 - +0.1 2,611,684 + +0.5 2,611,685 - -1.2 2,611,793 + -2.4 2,611,856 + ptrA HEPCGN_19300 0.11 +0.2 2,611,902 - ptrA HEPCGN_19300 0.13 -0.7 2,612,017 + ptrA HEPCGN_19300 0.17 -0.6 2,612,018 - ptrA HEPCGN_19300 0.17 -0.5 2,612,018 - ptrA HEPCGN_19300 0.17 -1.2 2,612,018 - ptrA HEPCGN_19300 0.17 -0.5 2,612,132 - ptrA HEPCGN_19300 0.21 +0.0 2,612,146 - ptrA HEPCGN_19300 0.21 +0.8 2,612,161 + ptrA HEPCGN_19300 0.22 -0.6 2,612,202 - ptrA HEPCGN_19300 0.23 +0.0 2,612,205 - ptrA HEPCGN_19300 0.23 -0.2 2,612,243 - ptrA HEPCGN_19300 0.25 +2.1
Or see this region's nucleotide sequence