Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygfX and fldB are separated by 39 nucleotides fldB and xerD are separated by 111 nucleotides xerD and dsbC are separated by 24 nucleotides dsbC and recJ are separated by 5 nucleotides
HEPCGN_18850: ygfX - Inner membrane protein YgfX, at 2,514,357 to 2,514,764
ygfX
HEPCGN_18855: fldB - flavodoxin FldB, at 2,514,804 to 2,515,325
fldB
HEPCGN_18860: xerD - site-specific tyrosine recombinase XerD, at 2,515,437 to 2,516,333
xerD
HEPCGN_18865: dsbC - bifunctional protein-disulfide isomerase/oxidoreductase DsbC, at 2,516,358 to 2,517,068
dsbC
HEPCGN_18870: recJ - single-stranded-DNA-specific exonuclease RecJ, at 2,517,074 to 2,518,807
recJ
Position (kb)
2515
2516
2517 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2514.440 kb on + strand, within ygfX at 2514.445 kb on + strand, within ygfX at 2514.490 kb on + strand, within ygfX at 2514.490 kb on + strand, within ygfX at 2514.490 kb on + strand, within ygfX at 2514.762 kb on + strand at 2514.781 kb on + strand at 2514.781 kb on + strand at 2514.782 kb on - strand at 2514.782 kb on - strand at 2514.782 kb on - strand at 2514.857 kb on + strand, within fldB at 2514.857 kb on + strand, within fldB at 2514.884 kb on + strand, within fldB at 2514.948 kb on - strand, within fldB at 2514.948 kb on - strand, within fldB at 2515.019 kb on + strand, within fldB at 2515.088 kb on + strand, within fldB at 2515.189 kb on + strand, within fldB at 2515.227 kb on - strand, within fldB at 2515.230 kb on + strand, within fldB at 2515.230 kb on + strand, within fldB at 2515.230 kb on + strand, within fldB at 2515.230 kb on + strand, within fldB at 2515.230 kb on + strand, within fldB at 2515.230 kb on + strand, within fldB at 2515.231 kb on - strand, within fldB at 2515.289 kb on - strand at 2515.289 kb on - strand at 2515.320 kb on - strand at 2515.320 kb on - strand at 2515.320 kb on - strand at 2515.325 kb on + strand at 2515.326 kb on - strand at 2515.330 kb on + strand at 2515.363 kb on + strand at 2515.363 kb on + strand at 2515.396 kb on + strand at 2515.403 kb on + strand at 2515.509 kb on - strand at 2515.637 kb on + strand, within xerD at 2515.775 kb on - strand, within xerD at 2515.863 kb on + strand, within xerD at 2515.863 kb on + strand, within xerD at 2515.863 kb on + strand, within xerD at 2515.863 kb on + strand, within xerD at 2515.863 kb on + strand, within xerD at 2515.863 kb on + strand, within xerD at 2515.864 kb on - strand, within xerD at 2515.864 kb on - strand, within xerD at 2515.864 kb on - strand, within xerD at 2515.949 kb on + strand, within xerD at 2515.949 kb on + strand, within xerD at 2516.118 kb on - strand, within xerD at 2516.201 kb on - strand, within xerD at 2516.236 kb on + strand, within xerD at 2516.285 kb on + strand at 2516.286 kb on - strand at 2516.373 kb on - strand at 2516.373 kb on - strand at 2516.378 kb on - strand at 2516.378 kb on - strand at 2516.378 kb on - strand at 2516.381 kb on + strand at 2516.381 kb on + strand at 2516.382 kb on - strand at 2516.382 kb on - strand at 2516.382 kb on - strand at 2516.382 kb on - strand at 2516.529 kb on + strand, within dsbC at 2516.529 kb on + strand, within dsbC at 2516.530 kb on - strand, within dsbC at 2516.530 kb on - strand, within dsbC at 2516.546 kb on + strand, within dsbC at 2516.546 kb on + strand, within dsbC at 2516.546 kb on + strand, within dsbC at 2516.547 kb on - strand, within dsbC at 2516.552 kb on + strand, within dsbC at 2516.552 kb on + strand, within dsbC at 2516.552 kb on + strand, within dsbC at 2516.553 kb on - strand, within dsbC at 2516.553 kb on - strand, within dsbC at 2516.571 kb on + strand, within dsbC at 2516.572 kb on - strand, within dsbC at 2516.572 kb on - strand, within dsbC at 2516.572 kb on - strand, within dsbC at 2516.572 kb on - strand, within dsbC at 2516.609 kb on + strand, within dsbC at 2516.609 kb on + strand, within dsbC at 2516.610 kb on - strand, within dsbC at 2516.610 kb on - strand, within dsbC at 2516.702 kb on + strand, within dsbC at 2516.748 kb on + strand, within dsbC at 2516.749 kb on - strand, within dsbC at 2516.775 kb on + strand, within dsbC at 2516.882 kb on + strand, within dsbC at 2517.067 kb on - strand at 2517.161 kb on + strand at 2517.177 kb on + strand at 2517.177 kb on + strand at 2517.214 kb on + strand at 2517.215 kb on - strand at 2517.267 kb on - strand, within recJ
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 2,514,440 + ygfX HEPCGN_18850 0.20 -1.1 2,514,445 + ygfX HEPCGN_18850 0.22 +0.4 2,514,490 + ygfX HEPCGN_18850 0.33 -0.4 2,514,490 + ygfX HEPCGN_18850 0.33 -1.9 2,514,490 + ygfX HEPCGN_18850 0.33 +1.1 2,514,762 + +0.4 2,514,781 + -0.5 2,514,781 + +0.5 2,514,782 - +0.6 2,514,782 - -1.7 2,514,782 - -0.1 2,514,857 + fldB HEPCGN_18855 0.10 +1.0 2,514,857 + fldB HEPCGN_18855 0.10 -1.0 2,514,884 + fldB HEPCGN_18855 0.15 -1.4 2,514,948 - fldB HEPCGN_18855 0.28 -1.2 2,514,948 - fldB HEPCGN_18855 0.28 +0.1 2,515,019 + fldB HEPCGN_18855 0.41 -0.9 2,515,088 + fldB HEPCGN_18855 0.54 -1.0 2,515,189 + fldB HEPCGN_18855 0.74 +3.5 2,515,227 - fldB HEPCGN_18855 0.81 -0.8 2,515,230 + fldB HEPCGN_18855 0.82 +0.1 2,515,230 + fldB HEPCGN_18855 0.82 -0.6 2,515,230 + fldB HEPCGN_18855 0.82 +0.2 2,515,230 + fldB HEPCGN_18855 0.82 -0.9 2,515,230 + fldB HEPCGN_18855 0.82 -1.3 2,515,230 + fldB HEPCGN_18855 0.82 -2.1 2,515,231 - fldB HEPCGN_18855 0.82 +1.5 2,515,289 - +1.4 2,515,289 - -0.1 2,515,320 - -0.7 2,515,320 - +1.3 2,515,320 - -0.0 2,515,325 + +0.6 2,515,326 - -0.2 2,515,330 + +0.3 2,515,363 + -1.9 2,515,363 + -0.1 2,515,396 + -0.6 2,515,403 + +0.4 2,515,509 - -0.8 2,515,637 + xerD HEPCGN_18860 0.22 +0.2 2,515,775 - xerD HEPCGN_18860 0.38 +1.3 2,515,863 + xerD HEPCGN_18860 0.47 +2.8 2,515,863 + xerD HEPCGN_18860 0.47 -0.3 2,515,863 + xerD HEPCGN_18860 0.47 -0.5 2,515,863 + xerD HEPCGN_18860 0.47 -2.0 2,515,863 + xerD HEPCGN_18860 0.47 -1.0 2,515,863 + xerD HEPCGN_18860 0.47 +2.9 2,515,864 - xerD HEPCGN_18860 0.48 -0.3 2,515,864 - xerD HEPCGN_18860 0.48 +1.6 2,515,864 - xerD HEPCGN_18860 0.48 +0.9 2,515,949 + xerD HEPCGN_18860 0.57 +1.1 2,515,949 + xerD HEPCGN_18860 0.57 +0.5 2,516,118 - xerD HEPCGN_18860 0.76 +0.1 2,516,201 - xerD HEPCGN_18860 0.85 +1.5 2,516,236 + xerD HEPCGN_18860 0.89 +1.4 2,516,285 + -1.6 2,516,286 - +1.4 2,516,373 - -0.5 2,516,373 - -0.8 2,516,378 - -0.1 2,516,378 - -0.1 2,516,378 - -0.1 2,516,381 + -0.5 2,516,381 + -0.6 2,516,382 - +0.2 2,516,382 - +0.6 2,516,382 - +1.3 2,516,382 - +0.1 2,516,529 + dsbC HEPCGN_18865 0.24 +0.2 2,516,529 + dsbC HEPCGN_18865 0.24 +1.7 2,516,530 - dsbC HEPCGN_18865 0.24 +1.4 2,516,530 - dsbC HEPCGN_18865 0.24 -0.1 2,516,546 + dsbC HEPCGN_18865 0.26 -1.1 2,516,546 + dsbC HEPCGN_18865 0.26 +1.8 2,516,546 + dsbC HEPCGN_18865 0.26 -1.4 2,516,547 - dsbC HEPCGN_18865 0.27 -0.4 2,516,552 + dsbC HEPCGN_18865 0.27 -0.7 2,516,552 + dsbC HEPCGN_18865 0.27 -0.5 2,516,552 + dsbC HEPCGN_18865 0.27 -0.5 2,516,553 - dsbC HEPCGN_18865 0.27 +0.3 2,516,553 - dsbC HEPCGN_18865 0.27 -1.6 2,516,571 + dsbC HEPCGN_18865 0.30 +0.2 2,516,572 - dsbC HEPCGN_18865 0.30 -0.2 2,516,572 - dsbC HEPCGN_18865 0.30 +0.6 2,516,572 - dsbC HEPCGN_18865 0.30 +0.8 2,516,572 - dsbC HEPCGN_18865 0.30 +0.0 2,516,609 + dsbC HEPCGN_18865 0.35 -0.2 2,516,609 + dsbC HEPCGN_18865 0.35 +0.0 2,516,610 - dsbC HEPCGN_18865 0.35 -0.9 2,516,610 - dsbC HEPCGN_18865 0.35 -0.2 2,516,702 + dsbC HEPCGN_18865 0.48 +2.9 2,516,748 + dsbC HEPCGN_18865 0.55 -0.9 2,516,749 - dsbC HEPCGN_18865 0.55 +0.4 2,516,775 + dsbC HEPCGN_18865 0.59 -0.6 2,516,882 + dsbC HEPCGN_18865 0.74 +0.7 2,517,067 - -0.8 2,517,161 + +0.5 2,517,177 + +0.6 2,517,177 + -1.4 2,517,214 + +1.5 2,517,215 - -0.5 2,517,267 - recJ HEPCGN_18870 0.11 +1.2
Or see this region's nucleotide sequence