Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvX and yqgE overlap by 1 nucleotides yqgE and gshB are separated by 36 nucleotides gshB and HEPCGN_18585 are separated by 12 nucleotides HEPCGN_18585 and endA are separated by 79 nucleotides
HEPCGN_18570: ruvX - Holliday junction resolvase RuvX, at 2,457,614 to 2,458,030
ruvX
HEPCGN_18575: yqgE - YqgE/AlgH family protein, at 2,458,030 to 2,458,665
yqgE
HEPCGN_18580: gshB - glutathione synthase, at 2,458,702 to 2,459,652
gshB
HEPCGN_18585: HEPCGN_18585 - Ribosomal RNA small subunit methyltransferase E, at 2,459,665 to 2,460,396
_18585
HEPCGN_18590: endA - deoxyribonuclease I, at 2,460,476 to 2,461,183
endA
Position (kb)
2458
2459
2460 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2458.129 kb on - strand, within yqgE at 2458.206 kb on - strand, within yqgE at 2458.206 kb on - strand, within yqgE at 2458.223 kb on - strand, within yqgE at 2458.223 kb on - strand, within yqgE at 2458.240 kb on - strand, within yqgE at 2458.342 kb on - strand, within yqgE at 2458.342 kb on - strand, within yqgE at 2458.506 kb on + strand, within yqgE at 2458.507 kb on - strand, within yqgE at 2458.507 kb on - strand, within yqgE at 2458.521 kb on - strand, within yqgE at 2458.521 kb on - strand, within yqgE at 2458.585 kb on + strand, within yqgE at 2458.586 kb on - strand, within yqgE at 2458.640 kb on - strand at 2458.714 kb on + strand at 2459.012 kb on - strand, within gshB at 2459.054 kb on + strand, within gshB at 2459.176 kb on - strand, within gshB at 2459.213 kb on - strand, within gshB at 2459.223 kb on + strand, within gshB at 2459.224 kb on - strand, within gshB at 2459.232 kb on + strand, within gshB at 2459.252 kb on - strand, within gshB at 2459.286 kb on + strand, within gshB at 2459.354 kb on + strand, within gshB at 2459.354 kb on + strand, within gshB at 2459.363 kb on + strand, within gshB at 2459.364 kb on - strand, within gshB at 2459.395 kb on - strand, within gshB at 2459.465 kb on + strand, within gshB at 2459.466 kb on - strand, within gshB at 2459.541 kb on + strand, within gshB at 2459.542 kb on - strand, within gshB at 2459.542 kb on - strand, within gshB at 2459.543 kb on + strand, within gshB at 2459.543 kb on + strand, within gshB at 2459.544 kb on - strand, within gshB at 2459.544 kb on - strand, within gshB at 2459.544 kb on - strand, within gshB at 2459.544 kb on - strand, within gshB at 2459.686 kb on + strand at 2459.755 kb on - strand, within HEPCGN_18585 at 2459.761 kb on - strand, within HEPCGN_18585 at 2459.856 kb on + strand, within HEPCGN_18585 at 2459.856 kb on + strand, within HEPCGN_18585 at 2459.856 kb on + strand, within HEPCGN_18585 at 2460.086 kb on - strand, within HEPCGN_18585 at 2460.115 kb on - strand, within HEPCGN_18585 at 2460.160 kb on - strand, within HEPCGN_18585 at 2460.378 kb on - strand at 2460.396 kb on + strand at 2460.396 kb on + strand at 2460.397 kb on - strand at 2460.432 kb on - strand at 2460.472 kb on + strand at 2460.510 kb on + strand at 2460.579 kb on + strand, within endA at 2460.580 kb on - strand, within endA at 2460.580 kb on - strand, within endA at 2460.636 kb on + strand, within endA at 2460.650 kb on - strand, within endA at 2460.652 kb on - strand, within endA at 2460.652 kb on - strand, within endA at 2460.652 kb on - strand, within endA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 2,458,129 - yqgE HEPCGN_18575 0.16 +2.0 2,458,206 - yqgE HEPCGN_18575 0.28 +0.8 2,458,206 - yqgE HEPCGN_18575 0.28 -0.7 2,458,223 - yqgE HEPCGN_18575 0.30 -0.1 2,458,223 - yqgE HEPCGN_18575 0.30 -0.1 2,458,240 - yqgE HEPCGN_18575 0.33 +1.0 2,458,342 - yqgE HEPCGN_18575 0.49 -0.3 2,458,342 - yqgE HEPCGN_18575 0.49 +0.0 2,458,506 + yqgE HEPCGN_18575 0.75 -0.8 2,458,507 - yqgE HEPCGN_18575 0.75 +0.5 2,458,507 - yqgE HEPCGN_18575 0.75 +0.3 2,458,521 - yqgE HEPCGN_18575 0.77 -0.1 2,458,521 - yqgE HEPCGN_18575 0.77 -0.2 2,458,585 + yqgE HEPCGN_18575 0.87 +0.1 2,458,586 - yqgE HEPCGN_18575 0.87 -1.0 2,458,640 - +0.9 2,458,714 + +0.3 2,459,012 - gshB HEPCGN_18580 0.33 -1.3 2,459,054 + gshB HEPCGN_18580 0.37 -0.5 2,459,176 - gshB HEPCGN_18580 0.50 -0.6 2,459,213 - gshB HEPCGN_18580 0.54 -0.8 2,459,223 + gshB HEPCGN_18580 0.55 -0.4 2,459,224 - gshB HEPCGN_18580 0.55 -0.2 2,459,232 + gshB HEPCGN_18580 0.56 -0.1 2,459,252 - gshB HEPCGN_18580 0.58 -0.4 2,459,286 + gshB HEPCGN_18580 0.61 +0.6 2,459,354 + gshB HEPCGN_18580 0.69 -2.0 2,459,354 + gshB HEPCGN_18580 0.69 -3.0 2,459,363 + gshB HEPCGN_18580 0.70 -1.1 2,459,364 - gshB HEPCGN_18580 0.70 -0.9 2,459,395 - gshB HEPCGN_18580 0.73 -2.2 2,459,465 + gshB HEPCGN_18580 0.80 -1.1 2,459,466 - gshB HEPCGN_18580 0.80 -1.2 2,459,541 + gshB HEPCGN_18580 0.88 -1.5 2,459,542 - gshB HEPCGN_18580 0.88 -2.5 2,459,542 - gshB HEPCGN_18580 0.88 -1.0 2,459,543 + gshB HEPCGN_18580 0.88 -1.9 2,459,543 + gshB HEPCGN_18580 0.88 -1.1 2,459,544 - gshB HEPCGN_18580 0.89 +0.6 2,459,544 - gshB HEPCGN_18580 0.89 -1.3 2,459,544 - gshB HEPCGN_18580 0.89 +0.4 2,459,544 - gshB HEPCGN_18580 0.89 -2.0 2,459,686 + +0.5 2,459,755 - HEPCGN_18585 0.12 +2.0 2,459,761 - HEPCGN_18585 0.13 +0.5 2,459,856 + HEPCGN_18585 0.26 +0.0 2,459,856 + HEPCGN_18585 0.26 +0.6 2,459,856 + HEPCGN_18585 0.26 +0.9 2,460,086 - HEPCGN_18585 0.58 -1.2 2,460,115 - HEPCGN_18585 0.61 +1.4 2,460,160 - HEPCGN_18585 0.68 -1.3 2,460,378 - +0.9 2,460,396 + +0.6 2,460,396 + -0.7 2,460,397 - -1.8 2,460,432 - -1.4 2,460,472 + -0.1 2,460,510 + -0.0 2,460,579 + endA HEPCGN_18590 0.15 -1.0 2,460,580 - endA HEPCGN_18590 0.15 -0.4 2,460,580 - endA HEPCGN_18590 0.15 +1.7 2,460,636 + endA HEPCGN_18590 0.23 +0.6 2,460,650 - endA HEPCGN_18590 0.25 +0.1 2,460,652 - endA HEPCGN_18590 0.25 -1.8 2,460,652 - endA HEPCGN_18590 0.25 +0.2 2,460,652 - endA HEPCGN_18590 0.25 +0.5
Or see this region's nucleotide sequence