Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hybC and hybD overlap by 1 nucleotides hybD and hybE overlap by 8 nucleotides hybE and hypA overlap by 8 nucleotides hypA and hybG are separated by 12 nucleotides hybG and yghU are separated by 69 nucleotides
HEPCGN_17880: hybC - hydrogenase 2 large subunit, at 2,320,928 to 2,322,631
hybC
HEPCGN_17885: hybD - HyaD/HybD family hydrogenase maturation endopeptidase, at 2,322,631 to 2,323,125
hybD
HEPCGN_17890: hybE - hydrogenase-2 assembly chaperone, at 2,323,118 to 2,323,606
hybE
HEPCGN_17895: hypA - hydrogenase maturation nickel metallochaperone HypA, at 2,323,599 to 2,323,940
hypA
HEPCGN_17900: hybG - hydrogenase maturation factor HybG, at 2,323,953 to 2,324,201
hybG
HEPCGN_17905: yghU - glutathione-dependent disulfide-bond oxidoreductase, at 2,324,271 to 2,325,137
yghU
Position (kb)
2323
2324 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2322.630 kb on - strand at 2322.630 kb on - strand at 2322.641 kb on + strand at 2322.718 kb on + strand, within hybD at 2322.719 kb on - strand, within hybD at 2322.734 kb on - strand, within hybD at 2322.888 kb on + strand, within hybD at 2322.929 kb on + strand, within hybD at 2322.930 kb on - strand, within hybD at 2323.098 kb on + strand at 2323.239 kb on - strand, within hybE at 2323.239 kb on - strand, within hybE at 2323.382 kb on + strand, within hybE at 2323.390 kb on + strand, within hybE at 2323.399 kb on + strand, within hybE at 2323.431 kb on - strand, within hybE at 2323.431 kb on - strand, within hybE at 2323.466 kb on - strand, within hybE at 2323.784 kb on - strand, within hypA at 2323.787 kb on + strand, within hypA at 2323.787 kb on + strand, within hypA at 2323.788 kb on - strand, within hypA at 2323.788 kb on - strand, within hypA at 2323.925 kb on + strand at 2323.949 kb on - strand at 2323.959 kb on - strand at 2323.997 kb on + strand, within hybG at 2324.023 kb on + strand, within hybG at 2324.023 kb on + strand, within hybG at 2324.024 kb on - strand, within hybG at 2324.024 kb on - strand, within hybG at 2324.024 kb on - strand, within hybG at 2324.024 kb on - strand, within hybG at 2324.030 kb on + strand, within hybG at 2324.069 kb on + strand, within hybG at 2324.069 kb on + strand, within hybG at 2324.070 kb on - strand, within hybG at 2324.104 kb on + strand, within hybG at 2324.159 kb on - strand, within hybG at 2324.182 kb on - strand at 2324.206 kb on + strand at 2324.244 kb on + strand at 2324.273 kb on - strand at 2324.285 kb on + strand at 2324.286 kb on - strand at 2324.364 kb on - strand, within yghU at 2324.429 kb on + strand, within yghU at 2324.429 kb on + strand, within yghU at 2324.430 kb on - strand, within yghU at 2324.430 kb on - strand, within yghU at 2324.430 kb on - strand, within yghU at 2324.431 kb on + strand, within yghU at 2324.432 kb on - strand, within yghU at 2324.464 kb on + strand, within yghU at 2324.465 kb on - strand, within yghU at 2324.601 kb on - strand, within yghU at 2324.717 kb on + strand, within yghU at 2324.767 kb on + strand, within yghU at 2324.810 kb on + strand, within yghU
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 2,322,630 - -0.2 2,322,630 - +2.0 2,322,641 + -0.0 2,322,718 + hybD HEPCGN_17885 0.18 -0.2 2,322,719 - hybD HEPCGN_17885 0.18 -0.9 2,322,734 - hybD HEPCGN_17885 0.21 +0.8 2,322,888 + hybD HEPCGN_17885 0.52 +0.5 2,322,929 + hybD HEPCGN_17885 0.60 +0.4 2,322,930 - hybD HEPCGN_17885 0.60 -0.4 2,323,098 + -0.7 2,323,239 - hybE HEPCGN_17890 0.25 -0.4 2,323,239 - hybE HEPCGN_17890 0.25 -2.2 2,323,382 + hybE HEPCGN_17890 0.54 +0.1 2,323,390 + hybE HEPCGN_17890 0.56 -1.3 2,323,399 + hybE HEPCGN_17890 0.57 -0.2 2,323,431 - hybE HEPCGN_17890 0.64 -0.8 2,323,431 - hybE HEPCGN_17890 0.64 -0.1 2,323,466 - hybE HEPCGN_17890 0.71 +0.5 2,323,784 - hypA HEPCGN_17895 0.54 -0.4 2,323,787 + hypA HEPCGN_17895 0.55 +0.2 2,323,787 + hypA HEPCGN_17895 0.55 +1.2 2,323,788 - hypA HEPCGN_17895 0.55 +2.5 2,323,788 - hypA HEPCGN_17895 0.55 -0.5 2,323,925 + -0.4 2,323,949 - -0.5 2,323,959 - -0.3 2,323,997 + hybG HEPCGN_17900 0.18 +1.1 2,324,023 + hybG HEPCGN_17900 0.28 +0.5 2,324,023 + hybG HEPCGN_17900 0.28 -0.6 2,324,024 - hybG HEPCGN_17900 0.29 +0.3 2,324,024 - hybG HEPCGN_17900 0.29 -0.2 2,324,024 - hybG HEPCGN_17900 0.29 -0.2 2,324,024 - hybG HEPCGN_17900 0.29 -1.0 2,324,030 + hybG HEPCGN_17900 0.31 +0.5 2,324,069 + hybG HEPCGN_17900 0.47 +0.2 2,324,069 + hybG HEPCGN_17900 0.47 +1.0 2,324,070 - hybG HEPCGN_17900 0.47 -2.2 2,324,104 + hybG HEPCGN_17900 0.61 -0.3 2,324,159 - hybG HEPCGN_17900 0.83 +0.3 2,324,182 - +0.3 2,324,206 + -0.6 2,324,244 + +1.2 2,324,273 - +1.2 2,324,285 + +1.2 2,324,286 - -0.7 2,324,364 - yghU HEPCGN_17905 0.11 +1.2 2,324,429 + yghU HEPCGN_17905 0.18 +0.1 2,324,429 + yghU HEPCGN_17905 0.18 +0.2 2,324,430 - yghU HEPCGN_17905 0.18 -0.6 2,324,430 - yghU HEPCGN_17905 0.18 -2.3 2,324,430 - yghU HEPCGN_17905 0.18 -0.2 2,324,431 + yghU HEPCGN_17905 0.18 -0.7 2,324,432 - yghU HEPCGN_17905 0.19 -0.3 2,324,464 + yghU HEPCGN_17905 0.22 +0.5 2,324,465 - yghU HEPCGN_17905 0.22 +1.2 2,324,601 - yghU HEPCGN_17905 0.38 +0.3 2,324,717 + yghU HEPCGN_17905 0.51 +0.5 2,324,767 + yghU HEPCGN_17905 0.57 -0.6 2,324,810 + yghU HEPCGN_17905 0.62 +0.1
Or see this region's nucleotide sequence