Strain Fitness in Escherichia coli ECOR38 around HEPCGN_17895

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthybC and hybD overlap by 1 nucleotideshybD and hybE overlap by 8 nucleotideshybE and hypA overlap by 8 nucleotideshypA and hybG are separated by 12 nucleotideshybG and yghU are separated by 69 nucleotides HEPCGN_17880: hybC - hydrogenase 2 large subunit, at 2,320,928 to 2,322,631 hybC HEPCGN_17885: hybD - HyaD/HybD family hydrogenase maturation endopeptidase, at 2,322,631 to 2,323,125 hybD HEPCGN_17890: hybE - hydrogenase-2 assembly chaperone, at 2,323,118 to 2,323,606 hybE HEPCGN_17895: hypA - hydrogenase maturation nickel metallochaperone HypA, at 2,323,599 to 2,323,940 hypA HEPCGN_17900: hybG - hydrogenase maturation factor HybG, at 2,323,953 to 2,324,201 hybG HEPCGN_17905: yghU - glutathione-dependent disulfide-bond oxidoreductase, at 2,324,271 to 2,325,137 yghU Position (kb) 2323 2324Strain fitness (log2 ratio) -2 -1 0 1 2at 2322.630 kb on - strandat 2322.630 kb on - strandat 2322.641 kb on + strandat 2322.718 kb on + strand, within hybDat 2322.719 kb on - strand, within hybDat 2322.734 kb on - strand, within hybDat 2322.888 kb on + strand, within hybDat 2322.929 kb on + strand, within hybDat 2322.930 kb on - strand, within hybDat 2323.098 kb on + strandat 2323.239 kb on - strand, within hybEat 2323.239 kb on - strand, within hybEat 2323.382 kb on + strand, within hybEat 2323.390 kb on + strand, within hybEat 2323.399 kb on + strand, within hybEat 2323.431 kb on - strand, within hybEat 2323.431 kb on - strand, within hybEat 2323.466 kb on - strand, within hybEat 2323.784 kb on - strand, within hypAat 2323.787 kb on + strand, within hypAat 2323.787 kb on + strand, within hypAat 2323.788 kb on - strand, within hypAat 2323.788 kb on - strand, within hypAat 2323.925 kb on + strandat 2323.949 kb on - strandat 2323.959 kb on - strandat 2323.997 kb on + strand, within hybGat 2324.023 kb on + strand, within hybGat 2324.023 kb on + strand, within hybGat 2324.024 kb on - strand, within hybGat 2324.024 kb on - strand, within hybGat 2324.024 kb on - strand, within hybGat 2324.024 kb on - strand, within hybGat 2324.030 kb on + strand, within hybGat 2324.069 kb on + strand, within hybGat 2324.069 kb on + strand, within hybGat 2324.070 kb on - strand, within hybGat 2324.104 kb on + strand, within hybGat 2324.159 kb on - strand, within hybGat 2324.182 kb on - strandat 2324.206 kb on + strandat 2324.244 kb on + strandat 2324.273 kb on - strandat 2324.285 kb on + strandat 2324.286 kb on - strandat 2324.364 kb on - strand, within yghUat 2324.429 kb on + strand, within yghUat 2324.429 kb on + strand, within yghUat 2324.430 kb on - strand, within yghUat 2324.430 kb on - strand, within yghUat 2324.430 kb on - strand, within yghUat 2324.431 kb on + strand, within yghUat 2324.432 kb on - strand, within yghUat 2324.464 kb on + strand, within yghUat 2324.465 kb on - strand, within yghUat 2324.601 kb on - strand, within yghUat 2324.717 kb on + strand, within yghUat 2324.767 kb on + strand, within yghUat 2324.810 kb on + strand, within yghU

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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2,322,630 - -0.2
2,322,630 - +2.0
2,322,641 + -0.0
2,322,718 + hybD HEPCGN_17885 0.18 -0.2
2,322,719 - hybD HEPCGN_17885 0.18 -0.9
2,322,734 - hybD HEPCGN_17885 0.21 +0.8
2,322,888 + hybD HEPCGN_17885 0.52 +0.5
2,322,929 + hybD HEPCGN_17885 0.60 +0.4
2,322,930 - hybD HEPCGN_17885 0.60 -0.4
2,323,098 + -0.7
2,323,239 - hybE HEPCGN_17890 0.25 -0.4
2,323,239 - hybE HEPCGN_17890 0.25 -2.2
2,323,382 + hybE HEPCGN_17890 0.54 +0.1
2,323,390 + hybE HEPCGN_17890 0.56 -1.3
2,323,399 + hybE HEPCGN_17890 0.57 -0.2
2,323,431 - hybE HEPCGN_17890 0.64 -0.8
2,323,431 - hybE HEPCGN_17890 0.64 -0.1
2,323,466 - hybE HEPCGN_17890 0.71 +0.5
2,323,784 - hypA HEPCGN_17895 0.54 -0.4
2,323,787 + hypA HEPCGN_17895 0.55 +0.2
2,323,787 + hypA HEPCGN_17895 0.55 +1.2
2,323,788 - hypA HEPCGN_17895 0.55 +2.5
2,323,788 - hypA HEPCGN_17895 0.55 -0.5
2,323,925 + -0.4
2,323,949 - -0.5
2,323,959 - -0.3
2,323,997 + hybG HEPCGN_17900 0.18 +1.1
2,324,023 + hybG HEPCGN_17900 0.28 +0.5
2,324,023 + hybG HEPCGN_17900 0.28 -0.6
2,324,024 - hybG HEPCGN_17900 0.29 +0.3
2,324,024 - hybG HEPCGN_17900 0.29 -0.2
2,324,024 - hybG HEPCGN_17900 0.29 -0.2
2,324,024 - hybG HEPCGN_17900 0.29 -1.0
2,324,030 + hybG HEPCGN_17900 0.31 +0.5
2,324,069 + hybG HEPCGN_17900 0.47 +0.2
2,324,069 + hybG HEPCGN_17900 0.47 +1.0
2,324,070 - hybG HEPCGN_17900 0.47 -2.2
2,324,104 + hybG HEPCGN_17900 0.61 -0.3
2,324,159 - hybG HEPCGN_17900 0.83 +0.3
2,324,182 - +0.3
2,324,206 + -0.6
2,324,244 + +1.2
2,324,273 - +1.2
2,324,285 + +1.2
2,324,286 - -0.7
2,324,364 - yghU HEPCGN_17905 0.11 +1.2
2,324,429 + yghU HEPCGN_17905 0.18 +0.1
2,324,429 + yghU HEPCGN_17905 0.18 +0.2
2,324,430 - yghU HEPCGN_17905 0.18 -0.6
2,324,430 - yghU HEPCGN_17905 0.18 -2.3
2,324,430 - yghU HEPCGN_17905 0.18 -0.2
2,324,431 + yghU HEPCGN_17905 0.18 -0.7
2,324,432 - yghU HEPCGN_17905 0.19 -0.3
2,324,464 + yghU HEPCGN_17905 0.22 +0.5
2,324,465 - yghU HEPCGN_17905 0.22 +1.2
2,324,601 - yghU HEPCGN_17905 0.38 +0.3
2,324,717 + yghU HEPCGN_17905 0.51 +0.5
2,324,767 + yghU HEPCGN_17905 0.57 -0.6
2,324,810 + yghU HEPCGN_17905 0.62 +0.1

Or see this region's nucleotide sequence