Experiment: Bas21
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sgcC and HEPCGN_15430 are separated by 73 nucleotides HEPCGN_15430 and ptsN overlap by 4 nucleotides ptsN and mngR are separated by 24 nucleotides mngR and nikE are separated by 198 nucleotides nikE and nikE are separated by 4 nucleotides
HEPCGN_15425: sgcC - PTS galactitol transporter subunit IIC, at 1,849,919 to 1,851,277
sgcC
HEPCGN_15430: HEPCGN_15430 - PTS sugar transporter subunit IIB, at 1,851,351 to 1,851,632
_15430
HEPCGN_15435: ptsN - PTS suar transporter subunit IIA, at 1,851,629 to 1,852,102
ptsN
HEPCGN_15440: mngR - GntR family transcriptional regulator, at 1,852,127 to 1,852,873
mngR
HEPCGN_15445: nikE - nickel import ATP-binding protein NikE, at 1,853,072 to 1,853,473
nikE
HEPCGN_15450: nikE - nickel import ATP-binding protein NikE, at 1,853,478 to 1,854,284
nikE
Position (kb) 1852
1853 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1851.235 kb on + strand at 1851.277 kb on + strand at 1851.410 kb on + strand, within HEPCGN_15430 at 1851.632 kb on + strand at 1851.665 kb on + strand at 1851.709 kb on + strand, within ptsN at 1851.710 kb on - strand, within ptsN at 1851.892 kb on + strand, within ptsN at 1851.949 kb on - strand, within ptsN at 1852.610 kb on + strand, within mngR at 1852.744 kb on + strand, within mngR at 1852.744 kb on + strand, within mngR at 1852.805 kb on - strand at 1852.814 kb on + strand at 1852.858 kb on + strand at 1852.862 kb on + strand at 1852.892 kb on + strand at 1852.892 kb on + strand at 1852.892 kb on + strand at 1852.902 kb on + strand at 1852.930 kb on + strand at 1852.930 kb on + strand at 1852.930 kb on + strand at 1852.930 kb on + strand at 1852.930 kb on + strand at 1852.932 kb on + strand at 1852.932 kb on + strand at 1852.935 kb on + strand at 1852.936 kb on - strand at 1853.066 kb on + strand at 1853.067 kb on - strand at 1853.073 kb on + strand at 1853.074 kb on - strand at 1853.074 kb on - strand at 1853.296 kb on - strand, within nikE at 1853.296 kb on - strand, within nikE at 1853.296 kb on - strand, within nikE at 1853.296 kb on - strand, within nikE at 1853.301 kb on + strand, within nikE at 1853.302 kb on - strand, within nikE at 1853.302 kb on - strand, within nikE at 1853.302 kb on - strand, within nikE at 1853.302 kb on - strand, within nikE at 1853.400 kb on + strand, within nikE at 1853.400 kb on + strand, within nikE at 1853.401 kb on - strand, within nikE at 1853.461 kb on + strand at 1853.473 kb on + strand at 1853.474 kb on - strand at 1853.474 kb on - strand at 1853.474 kb on - strand at 1853.571 kb on - strand, within nikE at 1853.649 kb on + strand, within nikE at 1853.649 kb on + strand, within nikE at 1853.650 kb on - strand, within nikE at 1853.650 kb on - strand, within nikE at 1853.650 kb on - strand, within nikE at 1853.770 kb on - strand, within nikE
Per-strain Table
Position Strand Gene LocusTag Fraction Bas21 remove 1,851,235 + -0.9 1,851,277 + -0.9 1,851,410 + HEPCGN_15430 0.21 -0.9 1,851,632 + +0.4 1,851,665 + +1.0 1,851,709 + ptsN HEPCGN_15435 0.17 +0.8 1,851,710 - ptsN HEPCGN_15435 0.17 -0.9 1,851,892 + ptsN HEPCGN_15435 0.55 +1.2 1,851,949 - ptsN HEPCGN_15435 0.68 -1.7 1,852,610 + mngR HEPCGN_15440 0.65 +0.9 1,852,744 + mngR HEPCGN_15440 0.83 -1.0 1,852,744 + mngR HEPCGN_15440 0.83 +3.1 1,852,805 - +0.9 1,852,814 + -0.5 1,852,858 + -0.3 1,852,862 + -1.1 1,852,892 + -1.4 1,852,892 + +0.5 1,852,892 + +0.1 1,852,902 + +0.2 1,852,930 + -0.3 1,852,930 + +1.0 1,852,930 + +0.5 1,852,930 + +1.2 1,852,930 + -0.7 1,852,932 + -0.0 1,852,932 + +1.1 1,852,935 + -1.0 1,852,936 - +0.7 1,853,066 + -0.5 1,853,067 - +0.0 1,853,073 + +1.0 1,853,074 - -1.6 1,853,074 - +2.1 1,853,296 - nikE HEPCGN_15445 0.56 -2.9 1,853,296 - nikE HEPCGN_15445 0.56 +0.9 1,853,296 - nikE HEPCGN_15445 0.56 -2.1 1,853,296 - nikE HEPCGN_15445 0.56 -0.6 1,853,301 + nikE HEPCGN_15445 0.57 +0.3 1,853,302 - nikE HEPCGN_15445 0.57 +0.3 1,853,302 - nikE HEPCGN_15445 0.57 -0.0 1,853,302 - nikE HEPCGN_15445 0.57 -1.4 1,853,302 - nikE HEPCGN_15445 0.57 +1.0 1,853,400 + nikE HEPCGN_15445 0.82 -0.7 1,853,400 + nikE HEPCGN_15445 0.82 -1.1 1,853,401 - nikE HEPCGN_15445 0.82 +0.4 1,853,461 + -2.1 1,853,473 + +1.1 1,853,474 - +2.4 1,853,474 - -0.4 1,853,474 - -1.0 1,853,571 - nikE HEPCGN_15450 0.12 -0.2 1,853,649 + nikE HEPCGN_15450 0.21 +1.4 1,853,649 + nikE HEPCGN_15450 0.21 +0.5 1,853,650 - nikE HEPCGN_15450 0.21 -0.6 1,853,650 - nikE HEPCGN_15450 0.21 -0.0 1,853,650 - nikE HEPCGN_15450 0.21 -0.4 1,853,770 - nikE HEPCGN_15450 0.36 +0.1
Or see this region's nucleotide sequence